Comparison between PCR and virus isolation results
Comparison of Viral Isolation and Multiplex Real Time Reverse Transcription PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays
... syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A ⴙ B and Binax NOW RSV assays over two successive respiratory ...
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A comparison between CHROMagar, PCR-RFLP and PCR-FSP for identification of Candida species
... both isolation and identification of Candida species, based on the pigmentation of the colonies with different colors, which is due to different enzyme activities in Candida ...in comparison with other ...
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Simultaneous Detection and Typing of Influenza Viruses A and B by a Nested Reverse Transcription PCR: Comparison to Virus Isolation and Antigen Detection by Immunofluorescence and Optical Immunoassay (FLU OIA)
... and virus isolation methods were used as references for evaluation of the OIA ...A virus in concentrated cells from nasopharyngeal aspirates, the OIA test had a significantly lower sensitivity than ...
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Comparison of Commercial Real Time PCR Assays for Quantification of Epstein Barr Virus DNA
... Epstein-Barr virus (EBV)-associated ...real-time PCR: the RealArt EBV LC PCR kit and the LightCycler EBV quantification ...nested PCR samples were used as negative ...differences ...
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Reverse transcription PCR detection of LaCrosse virus in mosquitoes and comparison with enzyme immunoassay and virus isolation
... A reverse transcription-PCR (RT-PCR) assay was developed and compared with enzyme immunoassay (EIA) and virus isolation for detecting LaCrosse virus (LAC) in mosquito pools.. All three t[r] ...
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Comparison of PCR, Immunofluorescence Assay, and Pathogen Isolation for Diagnosis of Q Fever in Humans with Spontaneous Abortions
... real-time PCR, and IFA turned out to be better tests than pathogen isolation for the rapid and reliable diagnosis of Q fever in women with sponta- neous ...Real-time PCR, with specificity equal to ...
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Comparison of Quantitative Competitive PCR with LightCycler Based PCR for Measuring Epstein Barr Virus DNA Load in Clinical Specimens
... good results when plasmid DNA or cell-de- rived DNA was spiked in ...poor results were observed when unfractionated whole-blood samples were used as ...TaqMan PCR and that TaqMan PCR may be ...
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Comparison of Herpes Simplex Virus PCR with Culture for Virus Detection in Multisource Surface Swab Specimens from Neonates
... plex virus (HSV) culture or PCR for testing of swabs of the conjunctivae, mouth, na- sopharynx, and rectum (surface swabs) from ...to results of HSV PCR with those of HSV culture with surface ...
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Comparison of a loop mediated isothermal amplification for orf virus withquantitative real time PCR
... agreement between the LAMP and real-time PCR ...real-time PCR. However, it is important to consider that real-time PCR is a time-consuming proced- ure that requires expensive and relatively ...
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Development and comparison of a real-time PCR assay for detection of Dichelobacter nodosus with culturing and conventional PCR: harmonisation between three laboratories
... diagnosis. PCR-based detection using conventional PCR has been used at our institutes, but the method was laborious and there was a need for a faster, easier-to-interpret ...real-time PCR assay for ...
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Comparison between Two Amplification Sets for Molecular Diagnosis of Toxoplasmosis by Real Time PCR
... immunodeficiency virus-positive or grafted patients. PCR has clearly improved the diagnosis of toxoplas- mosis and is today an inescapable technique for revealing the presence of the parasite in clinical ...
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Comparison of a Multiplex Real Time PCR Assay with a Multiplex Luminex Assay for Influenza Virus Detection
... syncytial virus, metapneumovirus, influenza virus types A and B, parainfluenza virus types 1 to 4, coronavirus, rhinovirus, bocavirus, and ...influenza virus real-time RT-PCR panel used ...
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Comparison of Quantitative Reverse Transcription PCR to Viral Culture for Assessment of Respiratory Syncytial Virus Shedding
... correlation between RT-PCR and culture declined in the present challenge ...discrepancy between culture and RT-PCR in natural infection is the result of both variable specimen pro- cessing and ...
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Multicenter Comparison of Different Real Time PCR Assays for Quantitative Detection of Epstein Barr Virus
... Epstein-Barr virus (EBV) in peripheral blood is important for the diagnosis and man- agement of serious EBV diseases, including posttransplant lymphoproliferative ...of PCR- based methods are currently in ...
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Comparison of Real Time PCR and Antigen Assays for Detection of Hepatitis E Virus in Blood Donors
... In developing countries, a simple and nonlaborious test such as HEV-Ag, enabling early detection of viral infection prior to the occurrence of IgM antibodies, would enhance the management of HEV infection. It has been ...
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Comparison of Simplexa HSV 1 & 2 PCR with Culture, Immunofluorescence, and Laboratory Developed TaqMan PCR for Detection of Herpes Simplex Virus in Swab Specimens
... There were several limitations to our study. First, testing was performed on all samples received on study days, but due to staff- ing limitations, the study could not be performed every day during the 4-month study ...
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PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems
... two PCR systems suggested a potential difference in the ability of the primers to amplify specific ...other PCR method were subjected to a serial dilution ...both PCR and dot blot analysis ...
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Comparison of Real Time PCR Assays with Fluorescent Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
... real-time PCR requires less hands-on time per specimen than FA, which is ...Real-time PCR assays offer advantages over conventional PCR by pro- viding lower risk of false-positive results due ...
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Interlaboratory Comparison of Six Real Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA
... The variation in the methods used by different laboratories in this study might have generated inconsistencies regarding the estimation of the number of copies of BLV proviral DNA among them. Since BLV is a global ...
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Rapid PCR TB Testing Results in 68.5% Reduction in Unnecessary Isolation Days in Smear Positive Patients.
... conducted between Sept 2016 and December 2017. A Xpert® MTB/RIF PCR test was performed on all AFB sputum smear positive patients from the initial sputum specimen collected on hospital ...positive ...
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