Competitive PCR
Comparison of the ABI 7700 System (TaqMan) and Competitive PCR for Quantification of IS6110 DNA in Sputum during Treatment of Tuberculosis
... Competitive PCR is a standard method for the quantifica- tion of DNA and has been used successfully in a number of studies (6, 9, 21, 25, 29, ...of PCR and reverse transcription-PCR products ...
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Measurement of Epstein Barr Virus DNA Loads in Whole Blood and Plasma by TaqMan PCR and in Peripheral Blood Lymphocytes by Competitive PCR
... by competitive PCR (cPCR) has been shown to be useful in the diagnosis and management of EBV infection and EBV-asso- ciated posttransplant lymphoproliferative disease (PTLD) (2, 3, 9, ...TaqMan PCR ...
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Clinical Evaluation of an In House Reverse Transcription Competitive PCR for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... by competitive PCR (AMPLICOR HIV-1 MONITOR test; Roche) or quantitative nucleic acid se- quence-based amplification (Q-NASBA; Organon Teknika) and signal amplification by the branched-DNA assay (QUAN- ...
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Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
... Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV ...
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Detection of Aspergillus species DNA in bronchoalveolar lavage samples by competitive PCR
... A competitive PCR assay involving the use of bronchoalveolar lavage (BAL) samples for the diagnosis of invasive pulmonary aspergillosis (IPA) was ...internal competitive control. Of 28 ...
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Evaluation of a quantitative competitive PCR assay for measuring herpes simplex virus DNA content in genital tract secretions
... quantitative PCR-based assays be performed in a competitive format (1, ...6). Competitive strategies involve coamplification of a range of concentrations of a modified PCR product with the ...
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Identification of enteroviruses in clinical specimens by competitive PCR followed by genetic typing using sequence analysis
... using competitive PCR with control RNA that is similar to the RNA of interest (4, ...In competitive PCR, the ratio of the unknown product to the competitor product has been shown to remain ...
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Comparison of Quantitative Competitive PCR with LightCycler Based PCR for Measuring Epstein Barr Virus DNA Load in Clinical Specimens
... real-time PCR assay for EBV load ...trarapid PCR system based on real-time fluorimetric quantifi- cation of PCR ...of PCR products in this assays ensures high ...quantitative ...
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Monitoring of Epstein Barr Virus DNA Load in Peripheral Blood by Quantitative Competitive PCR
... outgrowth of transformed B cells ex vivo as a measure for EBV load. This method, however, is laborious, time-consuming, and not suitable for large-scale screening of patient samples. To overcome the disadvantages of the ...
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Application of Competitive PCR to Cerebrospinal Fluid Samples from Patients with Herpes Simplex Encephalitis
... Quantitative analyses of viral load in vivo have been shown to be useful in the evaluation of several human viral infections (4, 5, 14, 16, 21, 28, 36). QC-PCR has been tested as a means of evaluating antiviral ...
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Comparison of characteristics of Q beta replicase amplified assay with competitive PCR assay for Chlamydia trachomatis
... semiquantitative PCR and Q b replicase-amplified assays for detection of this ...The PCR assay was directed against the ...the PCR and Q b replicase assays was five elementary ...and PCR ...
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Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments
... A close relationship is now thought to exist between Helico- bacter pylori and peptic ulcer disease, gastritis, and certain other gastroduodenal diseases (1, 2, 10, 12, 21, 26, 31, 33). The diagnosis of H. pylori ...
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Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus
... quantitative assay were consistently 1 log higher than those obtained by the AMPLICOR HCV MONITOR RT-PCR (un- published observations). Quantitative RT-PCR usually detects smaller quantities of HCV RNA than ...
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Early cytokine and chemokine gene expression in lymph nodes of macaques infected with simian immunodeficiency virus is predictive of disease outcome and vaccine efficacy.
... Competitive PCR was used to evaluate the expression of cytokine, granzyme B, and chemokine genes in lymph nodes of macaques recently infected with the simian immunodeficiency virus (SIV) pathogenic molec- ...
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Acute regulation by insulin of phosphatidylinositol 3 kinase, Rad, Glut 4, and lipoprotein lipase mRNA levels in human muscle
... transcription- competitive PCR, we have measured the mRNAs encoding the two insulin receptor variants, the insulin receptor sub- strate-1, the p85 a subunit of phosphatidylinositol-3-kinase, Ras associated ...
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Gene expression of the renin angiotensin system in human tissues Quantitative analysis by the polymerase chain reaction
... samples (e.g., renal biopsies) obtained from patients, we established a competitive PCR assay for quantification of renin, using a 155-basepair deletion mutant of the human renin cDNA as an internal ...
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Differential Activity of Two Non-hrOrigins during Replication of the Baculovirus Autographa californica Nuclear Polyhedrosis Virus Genome
... in competitive PCRs. PCR amplification and competitor ...and competitive PCRs for the HindIII-K, ie-1, and polH regions are shown in Table ...3. Competitive PCR reveals greater ...
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Semi Quantitative PCR for Quantification of Hepatotoxic Cyanobacteria
... the competitive PCR method to quantify toxic and non-toxic Microcystis ...the competitive PCR technique was later as- sessed against a cell count by optical ...
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Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
... the competitive PCR with the same efficiency over 24–39 cycles ...subsequent competitive PCRs were carried out for 30 cycles, when the amplification was found to be lineal and the efficiency was ...
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Interleukin-12 p40 mRNA Expression in Bovine Leukemia Virus-Infected Animals: Increase in Alymphocytosis but Decrease in Persistent Lymphocytosis
... quantitative competitive PCR, we show that peripheral blood mononuclear cells from bovine leukemia virus-infected animals in the alymphocytotic stage of disease express an increased amount of IL-12 p40 ...
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