Complementation of the DsAflJ gene knockout mutant
A Targeted Gene Knockout in Drosophila
... pected, the wild-type 9-kb band was converted into two Our results address the concerns of whether this bands of 7 and 10 kb (Figure 4). Hybridization with method can be used for targeted mutagenesis. We gen- a white ...
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The Cloning by Complementation of the pawn-A Gene in Paramecium
... pwA) was injected with total wild-type DNA or a fractional library of DNA, its clonal descendants all responded to stimuli like the wild type. By sorting a fractional library, we cloned and sequenced a 2.3-kb fragment ...
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Transcriptomics and knockout mutant analysis of rhizobacteria-mediated induced systemic resistance in Arabidopsis
... analyzed knockout mutants of a subset of these ...the gene by T-DNA insertion 15 base pairs downstream from the start codon rendered the knockout mutant plants incapable of mounting ...
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Complementation of a Herpes Simplex Virus ICP0 Null Mutant by Varicella-Zoster Virus ORF61p
... the gene are sensitive to interferon, as they fail to direct degradation of promyelocytic leukemia protein (PML), a component of host nuclear domain ...the gene for ICP0 replaced with sequences encoding ...
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Generation of medaka gene knockout models by target selected mutagenesis
... E241X mutant characterization We identified seven induced mutations in the medaka p53 gene [17], including three missense mutations, one splice site, and two nonsense mutations (Figure ...
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Isolation and Characterization of a New Peroxisome Deficient CHO Mutant Cell Belonging to Complementation Group 12
... PEX3 gene, giving rise to remaining of all of intron 6, thereby inducing 81 bp insertion between positions 523–524 of PEX3 ORF, resulting in deletion of 200 amino acid residues from the C-terminus of Pex3p and a ...
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Targeted knockout of a chemokine-like gene increases anxiety and fear responses
... A member of this family, sam2 exhibits exclusive brain-specific expression, predominantly in neurons of the dorsal Hb (dHb), as well as the telencephalic area and hypothalamus. Here, we re- port the development of a ...
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Cancer gene therapy using a survivin mutant adenovirus
... →Ala mutant retained the ability to physically associate with p34 cdc2 - cyclin B1 (21) and prevent phosphorylation of endoge- nous survivin ...of knockout studies (38), apoptosis induced by pAd-T34A did ...
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Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins
... the mutant F 1 animals showed small deletions at one site or deletions that spanned both sites, suggesting that the RGENs may have acted on both targeted sequences (Figure ...
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Inference of gene regulatory networks from genome-wide knockout fitness data
... genome-wide gene deletion/interruption technology along with the use of popula- tion fitness rather than gene expression as an observable offers novel challenges as well as benefits to the task of ...
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Functional complementation of pop2-3 T-DNA insertion mutant of Arabidopsis thaliana by overexpression of gamma-aminobutyric acid transaminase gene to reveals its role in complex pattern of leaf senescence
... GABA-T gene might release silencing of a subset of critical genes that regulating the leaf senescence ...pop2-3 mutant showed low chlorophyll content, increased ion leakage, and increased level of lipid ...
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Molecular Genetic Studies in Arabidopsis thaliana: I Cloning and Characterization of the Alcohol Dehydrogenase Gene; II Complementation of an Alcohol Dehydrogenase Mutant; III A Restriction Fragment Length Polymorphism Map of the Genome
... A Chlamydomonas reinhardtii gene for alpha-tubulin was used to isolate two homologous genes from Arabidopsis (Ludwig et al. The protein sequences have 90% identity. A number o[r] ...
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Studies of the susceptibility of nude, CD4 knockout, and SCID mutant mice to the disease induced by the murine AIDS defective virus.
... 2/2 knockout mice which lack CD4 1 T ...Ig gene rearrangement, expressing cytoplasmic C k RNA and being located in the peripheral lymphoid organs and not in the bone marrow (20, ...2/2 knockout mice, ...
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Deletion and Interallelic Complementation Analysis of Steel Mutant Mice
... function of Mgf. While the entire Mgfcoding region is deleted in SId' DNA, this allele contains the smallest deletion of the lethal alleles examined and is least likely to[r] ...
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Complementation of a Binding-Defective Retrovirus by a Host Cell Receptor Mutant
... stronger complementation of D84K virus infection by rK234D than by the rK234A receptor suggests that although the major reason for loss of infection of D84K and D84R virus is due to steric hindrance and charge ...
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A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout.
... (shRNA)-based gene silencing because, after several rounds of optimization, experimentally validated algorithms for selecting potent guide sequences have been developed ( Fellmann et ...
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A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout
... (shRNA)-based gene silencing because, after several rounds of optimization, experimentally validated algorithms for selecting potent guide sequences have been developed (Fellmann et ...
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Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus.
... ts-2, a temperature-sensitive and plaque morphology mutant of respiratory syncytial virus and sole representative of complementation group B, was com-.. pared with members of the other c[r] ...
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Complementation for replicative form DNA replication of a deletion mutant of H-1 by various parvoviruses.
... virus replication such as progeny DNA synthe- sis and encapsidation by a heterologous helper virus, dll was propagated in THK22A cells (Simian virus 40-transformed hamster kidney obtaine[r] ...
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Complementation of the rev gene mutation among human and simian lentiviruses.
... vitro-constructed rev and gag mutants of the following three primate lentiviruses: human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus from the African [r] ...
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