Conditioned media
Metabolically conditioned media derived from bone marrow stromal cells or human skin fibroblasts act as effective chemoattractants for mesenchymal stem cells
... with conditioned media derived from fibroblasts we needed to repeat BMSC migration (as summarized in ...control media of different cell sources induced different levels of cell migration themselves ...
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Bone conditioned media (BCM) improves osteoblast adhesion and differentiation on collagen barrier membranes
... bone conditioned media; BMP, bone morphogenetic protein; BSP, bone sialoprotein; EMD, enamel matrix derivative; ePTFE, expanded polytetrafluoroethylen membranes; FGF2, fibroblast growth factor 2; GAPDH, ...
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Myoblast-conditioned media improve regeneration and revascularization of ischemic muscles in diabetic mice
... with media from HO-1 overexpressing cells, while similarly lower in both animals treated with unconditioned media or that injected with conditioned media from control cells (Figure ...with ...
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Proteomic analysis of conditioned media from glucose responsive and glucose non responsive phenotypes reveals a panel of secreted proteins associated with beta cell dysfunction
... Media conditioned by dysfunctioning pancreatic beta cells offer an excellent source of potential protein markers associated with this ...in conditioned media (CM) from MIN-6(L) and MIN-6(H) ...
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KRAS mutant induces the release of the WSTF/NRG3 complex,
... (Figure 5C). HA-/MYC tagged WSTF/NRG3 was diluted after mixing with fresh media, whereas the total amount of WSTF/NRG3 was almost the same in the final media compared to the original media. Moreover, ...
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Augmented production of heparin binding mitogenic proteins by preadipocytes from massively obese persons
... deoxynucleotide for human bFGF. In the case of all cell strains, there was expression of two major bFGF transcripts, 7.0 and 3.7 kb. Although there was considerable variation in the degree of expression, preadipocytes ...
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Contact Chemoreception, Mating Behaviour, and Sexual Isolation in the Rotifer Genus Brachionus
... It is quite clear that the reaction of males in female-conditioned media is a real mating reaction for the following reasons: a Males exhibited the same type of reaction when transferred[r] ...
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Original Article Mucinous and serous ovarian carcinoma cells interact differently with stromal fibroblasts
... conditioned media of MRC-5 with Caov-3 or OMC685 were analyzed according to the manu- facturer’s ...different conditioned media at room temperature for 2 ...
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Salmonella typhimurium translocates flagellin across intestinal epithelia, inducing a proinflammatory response
... ponent. To ensure that the proteinase K was not inter- fering with the IL-8 induction assay (even though the proteinase K was inactivated by boiling), we verified that this proteinase had no effect on IL-8 induction by ...
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Cloning and identification of human Sca as a novel inhibitor of osteoclast formation and bone resorption
... of conditioned media from 293 cells transfected with the OIP- 1 cDNA clone confirmed that OIP-1 was released into the media as a membrane-bound GPI-linked ...
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Ell3 functions as a critical decision maker at the crossroad between stem cell senescence and apoptosis
... Abbreviations: C, control plasmid; Ell3 OE, Ell3-expressing plasmid; C-CM, conditioned media prepared from the culture media of ADSCs transfected with the control plasmid; OE-CM, conditi[r] ...
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A role for the primary cilium in paracrine signaling between mechanically stimulated osteocytes and mesenchymal stem cells
... Furthermore, conditioned media from flow stimulated osteoblasts did not significantly affect osteogenic gene expression in MSCs, suggesting that this novel mechanism is unique to osteocytes and ...
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Profiles of cytokines secreted by isolated human endometrial cells under the influence of chorionic gonadotropin during the window of embryo implantation
... the conditioned media of three- dimensional primary cell cultures of human endomet- rial epithelial cells, stromal cells, and epithelial plus stromal cells isolated from endometrial biopsies col- lected ...
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Integrated gene and miRNA expression analysis of prostate cancer associated fibroblasts supports a prominent role for interleukin-6 in fibroblast activation
... the conditioned media of primary normal fibroblasts activated in vitro with IL6 or TGFβ as well as those of patient-derived CAFs compared to the media obtained from non-activated HPFs, with a major ...
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Therapeutic Potential of Lung Spheroid Cell-Secreted Factors in Rodent Models of Pulmonary Fibrosis.
... To concentrate the samples, 15 ml of the conditioned media was lyophilized. The samples dried to completion in 24 h and were re-suspended in 1 ml of 100 mM ammonium bicarbonate (Sigma Aldrich) buffer at pH ...
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Activation of Astrocytes in Vitro by Macrophages Polarized with Keratin Biomaterial Treatment
... macrophage media produced no differences in reactive astrocyte-associated CSPG and GFAP expression (Figure 2 and Figure 3) and M1-conditioned media produced significant increases in GFAP (Figure 4 ...
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Role of ADAM17 in the non cell autonomous effects of oncogene induced senescence
... The conditioned media of the same cells as in (a), treated without doxycycline, were analyzed by label-free quantitative proteomics in triplicate ...
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Exosomes from IL 1β stimulated synovial fibroblasts induce osteoarthritic changes in articular chondrocytes
... SFB conditioned media (2 mL/well) were collected and cells were washed with PBS, and total RNA was isolated with TRIzol reagent (Life ...SFB-conditioned media (2 mL/well) were centrifuged for ...
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Stimulation of rat endothelial cell transforming growth factor beta production by bleomycin
... cell conditioned media were prepared in the presence of various doses of bleomycin, and tested for their ability to stimulate lung fibroblast collagen ...
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Autocrine and paracrine effects of atrial natriuretic peptide gene transfer on vascular smooth muscle and endothelial cellular growth
... of conditioned medium from endothelial cells transfected with ANP vector to nontransfected endothelial cells resulted in the significant increase in cyclic ...Similarly, conditioned media collected ...
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