Correlative light and electron microscopy
Investigating CNS synaptogenesis at single synapse resolution by combining reverse genetics with correlative light and electron microscopy
... for correlative light and 3D electron microscopy (3D ...scanning electron microscopy analysis of ms axons revealed T-bar-shaped dense bodies and other characteristic ...
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Visualization of HIV T Cell Virological Synapses and Virus-Containing Compartments by Three-Dimensional Correlative Light and Electron Microscopy
... fluorescence microscopy of cells infected with fluorescent HIV, we have visualized the formation of VS in live- or fixed-cell preparations to document the trafficking of viral proteins during VS formation ...
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Localization of active endogenous and exogenous beta-glucocerebrosidase by correlative light-electron microscopy in human fibroblasts
... by light micros- copy. 13 As fluorescence microscopy does not provide information about the underlying ultrastructure, in this study a correlative light and electron microscopy ...
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Ultrastructural Imaging of Salmonella-Host Interactions Using Super-resolution Correlative Light-Electron Microscopy of Bioorthogonal Pathogens
... struction microscopy (STORM) and placing this in a correlative light electron microscopy (CLEM) workflow, the pathogen can be imaged within its host cell context Typhimurium with a res- ...
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Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins
... Cryo super-resolution correlative light and electron microscopy on lipid nanotubes. We synthe- sised lipid nanotubes with 1 mol % Ni-NTA-conjugated lipids and labelled these with His-tagged ...
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Development of a new genetically encoded tag for correlative light electron microscopy
... 1 light oxygen voltage (LOV) domain can dimerize with an engineered PDZ domain following exposure to blue light (<500 ...a light-sensitive photosensory protein can bind cytochromes-interacting ...
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Regulation of Wnt/ß-catenin signalling by tankyrase-dependent poly(ADP-ribosyl)ation and scaffolding
... CLL/lymphoma 9-like protein; CK1, casein kinase 1; CLEM, correlative light and electron microscopy; CRC, colorectal cancer; DAX, polymerising domain present in Dishevelled an[r] ...
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FerriTag is a new genetically encoded inducible tag for correlative light electron microscopy
... Our first target protein for FerriTagging was clathrin. HeLa cells expressing FerriTag and clathrin light chain a (LCa) tagged with GFP and FKBP were imaged by fluorescence microscopy (Fig. 1b). These ...
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A correlative approach for combining microCT, light and transmission electron microscopy in a single 3D scenario
... include electron tomography, serial block face scanning electron micros- copy (SBF-SEM) (3View), focused ion bean scanning electron microscopy (FIB-SEM), and serial section TEM (see ...and ...
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Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells
... Fluorescence microscopy of GFP-tagged proteins is a fundamental tool in cell biology, but without seeing the structure of the surrounding cellular space, functional information can be ...with electron ...
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Dark side illuminated: imaging of Toxoplasma gondii through the decades
... Recently advances in SEM technology (and possibly more importantly computer and data handling) have facili- tated whole tissue sectioning and 3D reconstruction. Fixed tissue samples are serially sectioned and subjected ...
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Impact of Sildenafil on Smooth Muscle and Blood Vessels by Using Light and Electron Microscopy
... The diminished sinusoidal blood space due to the expanded collagen strands is demonstrated by recoloring areas and picture analyzer, or the expansion action of both fibroblasts and smooth muscles. The protein union ...
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Microscopical studies on the hemocytes of bivalves and their phagocytic interaction with selected bacteria
... The morphology of these cells, as determined by light, scanning and transmission electron microscopy, showed some similarity to mammalian- mononuclear phagocytes.. The sub-cel[r] ...
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Ultrastructural differences in pretangles between Alzheimer disease and corticobasal degeneration revealed by comparative light and electron microscopy
... Accurate identification of pretangles on LM, followed by EM examination of their exact counterpart was achieved through tau immunolabeling with QD, fluorescent nano- crystals, which are detectable with LM (fluorescence ...
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Gold nanoparticles as physiological markers of urine internalization into urothelial cells in vivo
... Under light microscopy (LM), the reaction product of silver enhancement was ...of electron-dense material in TEM contained elementary gold (Figure ...
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Vascular architecture of the human uterine cervix, as assessed in light and scanning electron microscopy
... until the smallest visible vessels of the perimetrium were filled with acrylic paint emulsion. After vessel closure the uteri were stored in a 10% solution of formaldehyde for two weeks. Next the specimens were sectioned ...
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Structural basis for control of secondary vessels in the long finned eel Anguilla reinhardtii
... several changes of distilled water and stained with 2% uranyl acetate for 30·min, and washed again. Tissues were then dehydrated in a graded series of ethanol (50, 70, 90, 95 and 100% for 10·min each, and then 1·h in ...
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Subcellular injury : ultrastructural changes induced in cultured cells by metabolic inhibitors and other agents
... l X Pages LIGHT MICROSCOPIC TECHNIQUES Phase Contrast Microscopy and Photomicrography 31 Fluorescent Microscopy 31 ELECTRON MICROSCOPIC TECHNIQUES Preparation of Cultured Cells for Elect[r] ...
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Electron microscopy of solids
... Even allowing for the absorption problem, which confront peaks at the low energy range of the spectrum, the peak size for N is very much lower than would be expected this is due to the interaction of the light ...
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FAMILIAL HEMATURIC NEPHRITIS
... tuna; seven of the family members were subjected to detailed laboratory studies and renal biopsy with both light and electron microscopy.. MATERIAL AND METHODS.[r] ...
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