cryo-EM
Volta phase plate cryo-EM of the small protein complex Prx3
... obtained cryo-EM reconstructions of hPrx3 filaments and observed a structured C terminus 11 , which has not been previously solved in homologous crystal structures, such as that of bovine Prx3 ...
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Blob ology and biology of cryo EM: an interview with Helen Saibil
... in cryo-EM data quality really kicked off the revolution: the software works much better, because the signal is so much bet- ter, giving a positive feedback because many structures started going to high ...
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High-resolution cryo-EM proteasome structures in drug development.
... crystallography has been pivotal in providing structural information for the development of proteasome inhibitors as therapeutic agents (Groll & Huber, 2004; Borissenko & Groll, 2007). However, the field of ...
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Cryo EM of amyloid fibrils and cellular aggregates
... single-particle cryo-EM have enabled the structure determination of amyloid fibrils from post-mortem human brain ...that cryo-EM allows near-atomic char- acterization of amyloid filaments from ...
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Blob-ology and biology of cryo-EM: an interview with Helen Saibil
... and cryo-EM fit into the current drive for reproducibility in science — data sharing, for example; and is the field working in the same direction in this respect? In single particle analysis this is fairly ...
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A microtubule RELION based pipeline for cryo EM image processing
... using cryo-electron microscopy (cryo-EM) is a powerful and widely applicable ...to cryo-EM for struc- ture ...of cryo-EM ...
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The 3.3 Å structure of a plant geminivirus using cryo-EM
... resolution cryo-EM studies on maize streak virus (MSV) 7 and African cassava mosaic virus (ACMV) 8 , combined with homol- ogy modeling, suggested that the geminivirus coat protein (CP) adopts a structure ...
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A2-Net: Molecular Structure Estimation from Cryo-EM Density Volumes
... from Cryo- Electron Microscopy (Cryo-EM) density volumes is the crit- ical last step of structure determination by Cryo-EM tech- ...3D Cryo-EM density ...
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X-ray and cryo-EM structures of inhibitor-bound cytochrome bc₁ complexes for structure-based drug discovery
... particle cryo-electron microscopy (cryo-EM), an increasing number of high-resolution structures have been determined, therefore the technique has the potential to play a role in SBDD programmes ...
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A cryo-EM grid preparation device for time-resolved structural studies
... in cryo-EM and has plenty of room for improvement both in terms of reproducibility and in the development of TrEM ...produce cryo-EM grids sufficient for high-resolution EM structure ...
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Cryo EM structure of lysenin pore elucidates membrane insertion by an aerolysin family protein
... Lysenin from the coelomic fluid of the earthworm Eisenia fetida belongs to the aerolysin family of small b-pore-forming toxins (b-PFTs), some members of which are pathogenic to humans and animals. Despite efforts, a ...
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Structural Insight into African Horsesickness Virus Infection
... The AHSV particle contains seven structural proteins (VP1 to VP7) arranged as three concentric layers surrounding the genome (5, 6, 23). Only the structure of a proteolytic fragment of VP7 has previously been reported ...
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At Low pH, Influenza Virus Matrix Protein M1 Undergoes a Conformational Change Prior to Dissociating from the Membrane
... by cryo-electron microscopy (cryo-EM), a technique in which native virion morphology is well ...in cryo-electron tomography (cryo-ET), which yields three-dimensional density maps of ...
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From DNA to proteins via the ribosome: Structural insights into the workings of the translation machinery
... of cryo-EM, the molecule is embedded in a thin ( 1,000 A ˚ ) vitr- eous layer of ice and kept at the temperature of liquid nitrogen for the duration of the experiment (see Figure ...3). ...
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The structure of an infectious human polyomavirus and its interactions with cellular receptors
... The structures of BKV presented here provide a detailed view of a native, infec- tious, human polyomavirus. The overall architecture of the BKV capsid is essentially the same as previ- ously described for SV40 and PyV. ...
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The Structural Architecture of an Infectious Mammalian Prion Using Electron Cryomicroscopy
... In the first single particle analysis approach, cryo-EM images were selected that presented clear Thon rings to enable correction of the contrast transfer function (CTF). In total, we extracted 1305 ...
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In Situ Structures of the Polymerase Complex and RNA Genome Show How Aquareovirus Transcription Machineries Respond to Uncoating
... used cryo-electron microscopy (cryo-EM) and a novel classification protocol based on our recent asymmetric-reconstruction method (13) to obtain asymmetric recon- structions of the ARV grass carp ...
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Epitope Insertion at the N-Terminal Molecular Switch of the Rabbit Hemorrhagic Disease Virus T=3 Capsid Protein Leads to Larger T=4 Capsids
... Pseudoatomic model of VP1 protein. Although there is con- siderable X-ray structural detail available for calicivirus CP, no lagovirus CP has been characterized at atomic resolution. VP1 protein was modeled using SMSV ...
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Glycan Shield and Fusion Activation of a Deltacoronavirus Spike Glycoprotein Fine-Tuned for Enteric Infections
... Sequence alignment of representative S glycoproteins from viruses of the four corona- virus genera shows that ␣ - and ␦ -coronaviruses feature a 14-residue-long insertion in the heptad repeat 1 (HR1) and HR2 motifs, ...
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Cryo-Electron Microscopy Structure of Lactococcal Siphophage 1358 Virion
... the EM density is broken between the tail core and the upper decoration, a sign of possible static disorder ...5 Cryo-EM reconstruction of the 1358 connector (17-Å resolution) and tail (16-Å ...
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