cryo-EM structure
Cryo EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein
... the cryo-EM and X-ray structures, small differences were found in ECL2 that forms part of the binding ...the cryo-EM structure this region is more dis- ...the cryo-EM ...
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Cryo EM structure of lysenin pore elucidates membrane insertion by an aerolysin family protein
... Members of the aerolysin family share an overall basic domain arrangement as evident by their crystal structures 2,3,5,9 (Fig. 4). They typically contain one or more receptor-binding domains and a PFM. The latter domain ...
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Cryo EM structure of the Ustilago maydis kinesin 5 motor domain bound to microtubules
... In many eukaryotes, kinesin-5 motors are essential for mitosis, and small molecules that inhibit human kinesin-5 disrupt cell division. To investigate whether fungal kinesin-5s could be targets for novel fungicides, we ...
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The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
... unsharpened map is deposited alongside EMD-3565). B-factor sharpening is used to improve high resolution features such as amino acid side chains, and simultaneously removes low resolution information such as the rel- ...
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Cryo EM of amyloid fibrils and cellular aggregates
... particle cryo-EM structure revealed the fold of soluble, full length huntingtin, which consists largely of HEAT repeats, but does not contain density for exon1, owing to disorder ...
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Differential Accessibility of a Rotavirus VP6 Epitope in Trimers Comprising Type I, II, or III Channels as Revealed by Binding of a Human Rotavirus VP6-Specific Antibody
... three-dimensional structure of VP6 (PDB: 1QHD) (13) to visualize the position of peptides identified by the DXMS ...the cryo-EM structure that the epitope is within two apical loops of the VP6 ...
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The structure of a β₂-microglobulin fibril suggests a molecular basis for its amyloid polymorphism
... L-shaped core formed by residues 22–85 (Fig. 3d). This con- formation is consistent with the MAS-NMR data, including a total of 1157 unique distance constraints (Fig. 3e; Supplementary Figure 3; Supplementary Tables ...
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PubMedCentral-PMC5501672.pdf
... Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene affect CFTR protein biogenesis or its function as a chloride channel, resulting in dysregula- tion of epithelial fluid transport in the ...
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Organization of Capsid-Associated Tegument Components in Kaposi's Sarcoma-Associated Herpesvirus
... resolution cryo-electron microscopy (cryo-EM) structure of Kaposi’s sarcoma-associated herpesvirus (KSHV) virion, a member of the oncogenic gammaherpesvirus ...virion structure reveals, ...
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Farnung, Lucas (2017): Nucleosome-Chd1 structure and implications for chromatin remodelling. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... high-resolution structure of a full-length chromatin remodeller bound to the ...The structure reveals an altered nucleosome where two helical turns of DNA are peeled away from the histone ...The ...
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X-ray and cryo-EM structures of inhibitor-bound cytochrome bc₁ complexes for structure-based drug discovery
... the cryo-EM structure has identified pyridone binding pose 2, which shows the aromatic ring facing in the opposite direction with the trifluoromethoxy group towards ...crystal structure reveal ...
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The 3.3 Å structure of a plant geminivirus using cryo-EM
... high-resolution structure for a geminivirus was available to describe how this architecture was ...resolution cryo-EM studies on maize streak virus (MSV) 7 and African cassava mosaic virus (ACMV) 8 , ...
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The structure of an infectious human polyomavirus and its interactions with cellular receptors
... high-resolution structure of the infectious BKV virion and how this interacts with its full repertoire of cellular ...microscopy structure of native, infectious BKV in complex with the receptor fragment of ...
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Limits of Structural Plasticity in a Picornavirus Capsid Revealed by a Massively Expanded Equine Rhinitis A Virus Particle
... the EM density well, the underside of the ERAV capsomere has a broad, circular depression in the elec- tron ...crystal structure, which is completely outside the envelope of the EM ...the ...
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The Structural Architecture of an Infectious Mammalian Prion Using Electron Cryomicroscopy
... the structure of the infectious prion protein, the infectious agent causing prion diseases such as sheep and goat scrapie, bovine spongiform encephalopathy or “mad cow disease ” , chronic wasting disease in ...
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Glycan Shield and Fusion Activation of a Deltacoronavirus Spike Glycoprotein Fine-Tuned for Enteric Infections
... S cryo-EM map ...S structure) preceded by a loop (poorly resolved in the HCoV-NL63 S ...X-ray structure (56), however, reveals that these polypeptide segments directly interact within the ...
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Structural Insight into African Horsesickness Virus Infection
... the structure of a proteolytic fragment of VP7 has previously been reported for AHSV ...atomic structure of any orbivirus, that of the BTV 1 core, revealed the organization of the genome and the two ...
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In Situ Structures of the Polymerase Complex and RNA Genome Show How Aquareovirus Transcription Machineries Respond to Uncoating
... RdRp VP2 has novel ligand interactions and an open template channel. The RdRp VP2 contains 1,274 residues organized into three domains: the N-terminal (amino acids [aa] 1 to 386) (NTD), core, and C-terminal bracelet (aa ...
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Structure of Flexible Filamentous Plant Viruses
... a structure of a given linear dimension (in fiber diffraction, the particle radius), the Fourier transform (the Fourier-Bessel transform in fiber diffraction) cannot oscillate at a frequency higher than that ...
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Structural Study of Piezo Channel, a Unique Family of Eukaryotic Mechanosensitive Channel
... first structure of a membrane protein, the TRPV1 ion channel, was solved at high resolution using single particle cryo-electron microscopy (cryo-EM) by the collaboration of David Julius’ and ...
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