cysteine residues
Pairs of Vp1 Cysteine Residues Essential for Simian Virus 40 Infection
... of cysteine pair, consisting of two different Vp1 cysteine residues, is required for forming the disulfide-linked monomer and oligomers observed ...the cysteine pair(s) nor their necessity ...
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Importance of Conserved Cysteine Residues in the Coronavirus Envelope Protein
... conserved cysteine residues are located adjacent to the carboxy side of the hydro- phobic, membrane-associated domain of all E proteins ...these residues are present at positions 40, 44, and 47 ...
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Relevance of cysteine residues for biosynthesis and antigenicity of human hepatitis B virus e protein.
... 1315-1321 0022-538X/93/031315-07$02.00/0 Copyright © 1993, American Society for Microbiology Relevance of Cysteine Residues for Biosynthesis and Antigenicity of Human Hepatitis B Virus e[r] ...
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Mutation of Neuraminidase Cysteine Residues Yields Temperature-Sensitive Influenza Viruses
... of cysteine residues in other systems has resulted in temperature-sensitive (ts) proteins, we asked whether mutation of cysteine residues in the influenza virus NA would yield ts ...of ...
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Role of Conserved Cysteine Residues in Hepatitis C Virus Glycoprotein E2 Folding and Function
... Simultaneous mutagenesis of cysteine pairs involved in di- sulfide formation. A Cys-to-Ala scan of the HIV envelope glycopro- tein gp120-gp41 complex revealed that mutations of individual cys- teines did not favor ...
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Functional contribution of cysteine residues to the human immunodeficiency virus type 1 envelope.
... HeLa CD4 cells were transfected with pHXB2D, the cysteine substitution mutants, or a noninfectious mutant defective in the integrase gene, which HIV-1 ENVELOPE CYSTEINE MUTANTS... TSCHAC[r] ...
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Mutational analysis of the cysteine residues in the hepatitis B virus small envelope protein.
... Of four mutants involving the eight cysteines located in the antigenic region, only the double mutant lacking Cys.121 and Cys-124 was secreted with wild-type efficiency.. Secretion-compe[r] ...
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Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris
... a cysteine res- idue (C1) and end shortly after the tenth cysteine residue (C10) at a (W/Y)X 6 (Y/F) motif (Figure ...additional cysteine residues after C10, and these were found to make ...
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Cysteines and N-Glycosylation Sites Conserved among All Alphaherpesviruses Regulate Membrane Fusion in Herpes Simplex Virus 1 Infection
... lar cysteine residues within the amino terminus of gK contributed to the regulation of HSV-1-induced membrane ...and cysteine mutant replication kinetics identified disparate effects on infectious ...
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Cysteine associated distribution of aromatic residues in disulfide stabilized extracellular protein families
... of cysteine residues providing critical disulfide stabiliza- tion of these four well-known modular motif ...The cysteine-aligned amino acid distribution data re- vealed limited ionic, polar, ...
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Cysteines in the Stalk of the Nipah Virus G Glycoprotein Are Located in a Distinct Subdomain Critical for Fusion Activation
... 9 Cysteine residues in the NiV-G stalk are located in a distinct domain important for fusion but not for physical F ...acid residues 71 to 188 from the reference sequences of NiV-G and HeV-G were ...
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Use of molecular modelling to probe the mechanism of the nucleoside transporter NupG
... duced cysteine residues to thiol reagents, and the roles of residues predicted from the model to be functionally important were investigated via site- directed mutagenesis and functional ...
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Covalent Modifications of the Ebola Virus Glycoprotein
... the cysteine residues in the extracellular domains of the GP is based on the fact that cysteines in extracellular domains are conserved exclusively for thiol-disulfide chemistry ...free cysteine in ...
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Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59.
... Gene 1 of the murine coronavirus, MHV-A59, encodes approximately 800 kDa of protein products within two overlapping open reading frames (ORFs 1a and 1b). The gene is expressed as a polyprotein that is processed into ...
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Characterization and modification of the carboxy-terminal sequences of bluetongue virus type 10 NS1 protein in relation to tubule formation and location of an antigenic epitope in the vicinity of the carboxy terminus of the protein.
... which residues of the carboxy-terminal se- quence are required, smaller deletion mutants were con- structed by removing just 5 or 10 amino acids from the carboxy terminus of NS1 (Materials and ...acid ...
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Human Equilibrative Nucleoside Transporter Subtype 1: Structure-Function Analysis Using Cysteine Mutagenesis and Thiol Modifying Techniques
... important residues in hENT1 structure and function include mutational techniques and chemical modifying ...a cysteine residue will react differently to sulfhydryl compounds of with various physiocochemical ...
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Palmitylation of the influenza virus hemagglutinin (H3) is not essential for virus assembly or infectivity.
... cysteine residues were replaced with various residues, and at- tempts to rescue transfectant viruses were ...for cysteine resi- dues equivalent to H3 subtype HA residues 555 and ...the ...
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Homo-Oligomerization of the Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein and the Role of Disulfide Linkages
... As a step toward understanding the assembly pathway of the porcine reproductive and respiratory syndrome virus (PRRSV), the oligomeric properties of the nucleocapsid (N) protein were investigated. In this study, we have ...
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Conserved Cysteines of the Human Immunodeficiency Virus Type 1 Protease Are Involved in Regulation of Polyprotein Processing and Viral Maturation of Immature Virions
... cysteine residues. Previous studies have shown that oxidation of cysteine 95 leads to inhibition of HIV-1 pro- tease activity while oxidation of cysteine 67 can either decrease or increase ...
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Analysis of viral diversity for vaccine target discovery
... default alignment parameters are used because the resulting alignment has to be visually inspected and manually corrected for misalignments, using either the BioEdit Sequence Alignment Editor [34, 35] or Jalview [36]. ...
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