Density gradient ultracentrifugation
Indicators of the atherogenic lipoprotein phenotype measured with density gradient ultracentrifugation predict changes in carotid intima-media thickness in men and women
... The present results cannot resolve this controversy, but can be interpreted as consistent with a role for TG-rich lipoproteins in early atherogenesis since VLDL-C and VLDL 1+2 -C concentrations were more strongly ...
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Original Article Identification and analysis of exosomes secreted from macrophages extracted by different methods
... (density gradient ultracentrifugation method, the ultracentrifuga- tion and ultrafiltration method, ExoQuick™ Extraction kit method) were used to collected exosomes in culture super- natants of ...
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Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis scleroderma overlap
... Autoantibodies in the serum from a patient with connective tissue disease have been used to define a high molecule weight acidic nuclear protein antigen. The antigen tentatively termed Ku, after the first two letters of ...
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Characterization of the putative wobbly possum disease virus : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Virology at Massey University, Manawatū, New Zealand
... iodixanol density gradient ultracentrifugation of infected cell culture lysates and the in vitro growth kinetics of WPDV in primary possum macrophages was investigated using a previously described ...
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Intestinal β galactosidases: II Biochemical alteration in human lactase deficiency
... 4.8. Density gradient ultracentrifugation of intestinal material from five lactase-deficient patients demonstrated absence of a lactase with pH optimum ...
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Surfactant Assisted Dispersion of Single-Walled Carbon Nanotubes in Polyvinylpyrrolidone Solutions
... High Purity and Yield Separation of Semiconducting Single-Walled Carbon Nanotubes Dispersed in Aqueous Solutions with Density Gradient Ultracentrifugation Using Mixed Dispersants of Po[r] ...
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Slowly Sedimenting Mercaptoethanol resistant Antinuclear Factors Related Antigenically to M Immunoglobulins (γ1M Globulin) in Patients with Systemic Lupus Erythematosus
... In sera Ro and Mu Figure 7, which had IgM antibody activity in the rapidly and slowly sedimenting fractions on sucrose density gradient ultracentrifugation, ANF that reacted with anti-Ig[r] ...
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Salmonella bacteriophage glycanases: endorhamnosidases of Salmonella typhimurium bacteriophages.
... In the instances where the phages were purified by density gradient ultracentrifugation, the glycanase activities were found associated with the phage particles, indicating that the enzy[r] ...
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Antibody formation in dysgammaglobulinemia
... The nature of the slowly sedimenting anti-OX activity in the 191-day serum was examined further by sucrose density gradient ultracentrifugation and immunodiffusion analysis with antisera[r] ...
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Biosynthesis of low molecular weight (7S) and high molecular weight (19S) immunoglobulin M
... The class of immunoglobulin M (IgM) characterized by high molecular weight proteins with a sedimentation coefficient of 19S, includes a smaller molecular form with an S 20,[unk] of approximately 7. The synthetic origin ...
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On the Estimation of the Gradient Lines of a Density and the Consistency of the Mean-Shift Algorithm
... Recall that a critical point of f is a point x at which the gradient of f vanishes, that is, such that ∇f(x) = 0. A flow line or integral curve of the positive gradient flow of f is a curve x such that x 0 ...
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A rapid and scalable density gradient purification method for Plasmodium sporozoites
... A previously published method of sporozoite purifica- tion requires that the parasites be first coated with bovine serum albumin (BSA) and then passed through ion ex- change resin [7]. While this described procedure ...
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Analytical Ultracentrifugation pdf
... concentration gradients using absorption or refrac- tometric optics. In sedimentation velocity experi- ments a moving boundary forms upon application of a high centrifugal force. The rate of movement of the boundary is ...
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THE PHOSPHOLIPID COMPOSITION OF HUMAN SERUM LIPOPROTEIN FRACTIONS SEPARATED BY ULTRACENTRIFUGATION
... Serum lipoprotein fractions of density < 1.019, < 1.063 and > 1.063 from eight apparently healthy fasting adults were separated by ultracentrifugation and analyzed for the individual pho[r] ...
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Identification and Characterization of the Lipid Transport System in the Tarantula Grammostola rosea
... a density gradient with NaBr ...a gradient of similar density (NaBr ...a density of 1.24 - 1.27 g/ml, and proteins with higher density are not ...
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New function for high density lipoproteins Isolation and characterization of a bacterial lipopolysaccharide high density lipoprotein complex formed in rabbit plasma
... The addition of bacterial lipopolysaccharide (LPS) from Salmonella minnesota R595 to rabbit plasma results in a marked reduction of the hydrated buoyant density of the parent R595 LPS, from 1.38 to less than 1.2 ...
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Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation
... By using rate-zonal centrifugation followed by tional Institutes of Health, USPHS, Minimum Reisopycnic banding, we previously demonstrated quirements: Influenza Virus Vaccines, Types A a[r] ...
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Multiple-Instabilities in Magnetized Plasmas with Density Gradient and Velocity Shears
... We systematically investigate the linear analyses of in- stabilities in the inhomogeneous magnetized plasmas with a density gradient, and parallel and perpendicular mean flows. The dispersion relation, ...
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Density Gradient Centrifugation of Rubella Virus
... As EDTA can be seen, the virus obtained from the heavier 1.18 g/cm3 band after 18 hr of centrifugation was B Virus centrifuged in sucrose found on recentrifugation to band at the lighter[r] ...
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Quantitation of RNA tumor viruses by spectroscopy of density gradient gels.
... Using 0.8-ml gradients gels 4 by 45 mm we can detect as low as 2 x 107 viral particles with Coomassie blue staining and 6 x 106 viral particles with a more sensitive staining procedure u[r] ...
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