dimerization / retrovirus / packaging / RNA structure
Is HIV 1 RNA dimerization a prerequisite for packaging? Yes, no, probably?
... the structure of these RNA genomes became a matter of great ...RSV RNA species could be converted to a 36S species by heat treatment suggested a disaggregation of the 62S RNA into smaller ...
14
Structural implications of RNA dimerization in a gamma retrovirus
... The RNA strands are then reverse transcribed into viral DNA and integrated into the host genome where the virus can remain in a dormant state for long ...genomic RNA. Interestingly, two genomic RNA ...
121
The dimerization/packaging sequence is dispensable for both the formation of high-molecular-weight RNA complexes within retroviral particles and the synthesis of proviruses of normal structure.
... genomic RNA molecules, linked by noncovalent intermolecular ...viral RNA extracted from virions as well as in vitro studies have implicated a sequence, designated the dimer linkage sequence (DLS), in the ...
6
RNA secondary structure and binding sites for gag gene products in the 5' packaging signal of human immunodeficiency virus type 1.
... HIV-1 RNA dimerization in ...that RNA oligonucleotides de- rived from the HIV-1 C region will dimerize spontaneously in vitro and that this dimerization is facilitated kinetically by Gag ...
9
Palindromic Sequence Plays a Critical Role in Human Foamy Virus Dimerization
... retroviral RNA genome is dimeric, consisting of two identical strands of RNA linked near their 5 ⴕ ends by a dimer linkage ...(HFV) RNA transcribed in vitro contained three sites, designated SI, SII, ...
9
Opening of the TAR hairpin in the HIV 1 genome causes aberrant RNA dimerization and packaging
... genomic RNA that is encapsidated and present as dimer in the viral ...this packaging process, the genomic RNA must be selected from a multitude of cellular and spliced viral ...cis-acting RNA ...
12
A dual role of the putative RNA dimerization initiation site of human immunodeficiency virus type 1 in genomic RNA packaging and proviral DNA synthesis.
... genomic RNA dimer formation and/or stability ...for RNA dimerization was found to have no effect ...the RNA genome or trans-acting factors are able to compensate the loop ...the ...
7
Structural basis of genomic RNA (gRNA) dimerization and packaging determinants of mouse mammary tumor virus (MMTV)
... specific packaging of two copies of retroviral gRNA in the form of a non-covalent RNA dimer by the assembling ...of dimerization is closely linked with gRNA packaging, and in some ...
21
Sequences Downstream of the 5′ Splice Donor Site Are Required for both Packaging and Dimerization of Human Immunodeficiency Virus Type 1 RNA
... viral RNA packaging and dimerization, we analyzed the local sec- ondary structure with the M-Fold algorithm (29, ...viral RNA dimerization defect was not corrected despite the ...
14
Mechanism of retroviral genomic RNA dimerization and packaging
... the RNA, indicating that this position is more constrained in the PAL1Stb ...PAL1 structure yields a prominent stop to primer extension by the reverse transcriptase enzyme (nts 223-229, dashed line in ...
126
Possible Role of Dimerization in Human Immunodeficiency Virus Type 1 Genome RNA Packaging
... the packaging and dimerization functions ...for dimerization and not for packaging, whereas we observed re- gions functional for packaging but not dimerization ...for ...
10
Role of the DIS hairpin in replication of human immunodeficiency virus type 1.
... unspliced RNA molecules. A hairpin structure within the untranslated leader transcript is postulated to play a role in RNA dimerization through base pairing of the autocomplementary loop ...
10
Mutations in the kissing-loop hairpin of human immunodeficiency virus type 1 reduce viral infectivity as well as genomic RNA packaging and dimerization.
... genomic RNA concentration high enough to bypass the RNA dimerization defect (by virtue of the law of mass action) or to produce a massive number of infected MT-4 cells from which one or a few will, ...
10
Structure of B77 sarcoma virus RNA: stabilization of RNA after packaging.
... Denaturation curves for RNA isolated by high-salt 0.5 M NaCl phenol extraction of virus harvested at various intervals from the medium of B77-infected cells are given in Fig.. 5 indicati[r] ...
10
Gene therapy strategies for treating brain tumors: Retroviruses are still good candidates for therapeutic vectors
... a retrovirus-producing cell line, PAMP51, that produces viruses (at a titer > 1 × 10 7 CFU/mL) by introduction of the polyoma early region into the retroviral genome ...of retrovirus directly into the ...
7
Moloney Murine Sarcoma Virus Genomic RNAs Dimerize via a Two-Step Process: a Concentration-Dependent Kissing-Loop Interaction Is Driven by Initial Contact between Consecutive Guanines
... vitro dimerization of Moloney murine sarcoma virus (MuSV) RNA in the context of these two ...vitro RNA dimerization and in vivo viral ...support dimerization at significantly higher ...
7
Secondary Structure of the Mature Ex Virio Moloney Murine Leukemia Virus Genomic RNA Dimerization Domain
... upon dimerization in vitro ...an RNA structure and also provide additional support for our overall model for the architecture of the dimerization ...
9
Retrovirus-Specific Differences in Matrix and Nucleocapsid Protein-Nucleic Acid Interactions: Implications for Genomic RNA Packaging
... gRNA packaging. The implications of these findings for specific viral RNA recognition by HTLV MA and MA’s role in the context FIG 8 HIV-1 gRNA packaging efficiency of the HIV-1 MA TRI-MUT variant in ...
10
Studies of the Genomic RNA of Leukosis Viruses: Implications for RNA Dimerization
... stable RNA dimer that dissociates at a higher temperature (17, ...the RNA conforma- tional change since in vitro experiments show that HIV-1 NC is capable of converting the transient kissing-loop complex ...
10
Nucleic Acid-Independent Retrovirus Assembly Can Be Driven by Dimerization
... In vivo, Gag protein is released from cells into the medium in a membrane-enclosed, particulate form, a process referred to as budding. Budding is not synonymous with assembly, which we define as the polymerization of ...
9