Displacement amplification
Caught in the middle with multiple displacement amplification: the myth of pooling for avoiding multiple displacement amplification bias in a metagenome
... Multiple displacement amplification (MDA) has been one of the most commonly used means of amplifying environmental genomic DNA (gDNA), especially viral gDNA, prior to the construction of DNA fragment se- ...
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Detection of Mycobacterium tuberculosis in respiratory specimens by strand displacement amplification of DNA
... A total of 294 clinical respiratory specimens, including 75 with culture-positive results, were tested for the presence of Mycobacterium tuberculosis by strand displacement amplification (SDA) of DNA. A ...
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Multiple displacement amplification as an adjunct to PCR based detection of Staphylococcus aureus in synovial fluid
... Background: Detection of bacterial nucleic acids in synovial fluid following total joint arthroplasty with suspected infection can be difficult; among other technical challenges, inhibitors in the specimens require ...
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Isothermal multiple displacement amplification: a methodical approach enhancing molecular routine diagnostics of microcarcinomas and small biopsies
... multiple displacement amplification (IMDA) can be a powerful tool in molecular routine diagnostics for homogeneous and sequence-independent whole-genome amplification of notably small tumor samples, ...
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Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of <em>Listeria monocytogenes</em>
... cross displacement amplification (MCDA) label-based gold nanoparticles lateral flow biosensor (LFB) for specific, sensitive, and visual detection of ...the amplification mixtures were directly ...
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Use of Multiple Displacement Amplification and Checkerboard DNA DNA Hybridization To Examine the Microbiota of Endodontic Infections
... Multiple-displacement amplification (MDA) has been used to uniformly amplify bacterial genomes present in small samples, providing abundant targets for molecular ...
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Detection of Viable Mycobacterium tuberculosis by Reverse Transcriptase Strand Displacement Amplification of mRNA
... peutic efficacy and/or susceptibility to antibacterial agents. We have previously described a method for the efficient recovery of mycobacterial RNA from clinical specimens, as well as the use of reverse transcriptase ...
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Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Strand Displacement Amplification and Relevance of the Amplification Control for Use with Vaginal Swab Specimens
... strand displacement amplification assay (SDA) with the BDProbeTec ET instrument and then to evaluate the use of the amplification control in a clinical research ...the amplification control ...
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Visual and rapid detection of Acinetobacter baumannii by a multiple cross displacement amplification combined with nanoparticles-based biosensor assay
... cross displacement amplification (MCDA) primers was designed to recognize 10 different regions of the pgaD gene, which was conservative and specific for the ...system, amplification primers D1 and R1 ...
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Confirmation of the presence of Mycobacterium tuberculosis and other mycobacteria in mycobacterial growth indicator tubes (MGIT) by multiplex strand displacement amplification
... Multiplex strand displacement amplification (mSDA) is capable of amplifying three distinct DNA sequences simultaneously. These include sequences present in most genera of mycobacteria, a sequence specific ...
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Use of Multiple Displacement Amplification as Pre polymerase Chain Reaction (Pre PCR) to amplify genomic DNA of siphonapterids preserved for long periods in scientific collections
... the amplification of whole genomes from small amounts of template ...multiple displacement amplification method (MDA), a process that uses the bacteriophage 29 DNA polymerase and ...
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Site-Specific Inelastic Displacement Amplification Factors
... inelastic displacement amplification factors, C1, calculated from ...frequency amplification and behavior that is not adequately captured by the ASCE 41 ...inelastic displacement ...
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Displacement amplification ratio modeling of bridge-type nano-positioners with input displacement loss
... actual displacement amplification ratio of the nano-positioner is ...the amplification performance of the bridge-type nano- positioner by considering the impact of input end displace- ment ...
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<p>Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detect <em>Neisseria meningitidis</em></p>
... More recently, multiple cross displacement ampli fi - cation (MCDA) was developed to overcome the techni- cal barriers posed by current isothermal ampli fi cation strategies. MCDA assay was conducted under isother- ...
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Rendering of mycobacteria safe for molecular diagnostic studies and development of a lysis method for strand displacement amplification and PCR
... The NALC pellets from the seven positive and seven negative clinical samples that were tested in neutralization experiments were lysed by 30 min of heating at 100°C in a forced-air oven,[r] ...
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SNES: single nucleus exome sequencing
... of amplification are inherited by all subsequent ...multiple-displacement- amplification (MDA) [9] and multiple-annealing-looping- based-amplification-cycles (MALBAC) [10] to ...
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Isothermal Amplification and Molecular Typing of the Obligate Intracellular Pathogen Mycobacterium leprae Isolated from Tissues of Unknown Origins
... multiple-displacement amplification to allow access to a high-quality DNA ...evaluated amplification fidelity, genome coverage, and regional amplification ...global amplification ...
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Improved Sensitivity of Nucleic Acid Amplification for Rapid Diagnosis of Tuberculous Meningitis
... strand displacement amplification method (the standard ProbeTec method) for the detection of Mycobacterium tuberculosis complex organisms in parallel with the ProbeTec method with a modified pretreatment ...
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Cross-amplification and characterization of microsatellite markers in Alcantarea patriae Versieux & Wand.
... Overall success in transferability was approximately 71% (22 of the 31 loci evaluated), with 54.5% of the markers transferred (12 of 22 loci) being polymorphic. Barbará et al. (2007) showed that the best results of ...
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Lagrangian characteristics of continental shelf flows forced by periodic wind stress
... Abstract. The coastal ocean may experience periods of fluc- tuating along-shelf wind direction, causing shifts between upwelling and downwelling conditions with responses that are not symmetric. We seek to understand ...
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