DNA Extraction and PCR amplification:
Methods for microbial DNA extraction from soil for PCR amplification
... of DNA-based techniques can overcome this limitation by allowing the fate of particular genes or organisms to be monitored directly in environmental ...extract DNA from soil and sediment initially used ...
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Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification
... Finally, as we have already mentioned, the main reason for optimising the DNA extraction protocol was to analyse parasite infection in Lutzomyia spp. by PCR amplification. In this sense, it is ...
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Simple and quick method of DNA extraction from different parts of plant for PCR amplification
... genomic DNA from plants for genetic analysis. Majority of currently used DNA extraction methods and commercially available kits are multi-stepped, time consuming, expensive especially when dealing ...
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Grapevine yellows: Comparison of different procedures for DNA extraction and amplification with PCR for routine diagnosis of phytoplasmas in grapevine
... plant DNA extraction and 4 procedures for amplifica- tion in polymerase chain reaction of phytoplasma DNA were examined in parallel using the same plant tissues infected with ...The DNA ...
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Bringing Laboulbeniales into the 21st century: enhanced techniques for extraction and PCR amplification of DNA from minute ectoparasitic fungi
... for extraction of Laboulbeniales DNA involves using freshly collected material preferably stored in 95 % ...our DNA isolation positive ...good DNA preservation for a prolonged period of time. ...
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Evaluation of DNA Extraction and PCR Methods for Detection of Enterocytozoon bienuesi in Stool Specimens
... The DNA pellet was resuspended in 200 l of TE buffer and kept at ⫺20°C until ...three DNA extraction methods, a stool specimen with a known concentration of microsporidial spores was ...extracted ...
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Evaluation of Simple and Cost-Effective DNA Preparation and Subsequent PCR Amplification for Clinically Relevant Mycobacteria
... 2.3 DNA Extraction and Quantification A total of 10 heat-treated MTB and 20NTM isolates were evaluated for 8 DNA extraction protocols that differed by the lysis solution used (Table ...each ...
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Integrated DNA extraction and amplification on a microfluidic device
... system. DNA extraction and pre-concentration was performed ‘off-chip’ using commercial ...The DNA sample was then manually injected into a 160 nl PCR chamber along with all the necessary ...
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The Inhibitory Effect of Mouse Gastric DNA on Amplification of Helicobacter pylori Genomic DNA in Quantitative PCR
... different extraction methods and found that the lowest inhibition is observed in samples homogenized by vortexing and purified by commercial column-based ...non-target DNA by amplification of ...
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Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
... from PCR- amplicon based assessments of biodiversity to the analysis of taxa and genes using random shotgun sequencing as this more holistic approach becomes more amenable for use by individual researchers into ...
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Influence of salts and pcr inhibitors on the amplification capacity of three thermostable DNA polymerases
... полимерази; PCR: PCR-инхибитори ...the amplification of nu- cleic acids in the PCR are often present with the target ...for extraction of DNA, they interfere by nucleic acid ...
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Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
... insect DNA have been ...the DNA of insect speci- mens is not ...extracted DNA from the specimens and ran both total DNA extracts and polymerase chain reaction (PCR) products on agarose ...
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Strategies for the Extraction, Purification and Amplification of Metagenomic DNA from Soil Growing Sugarcane
... environmental DNA [12]. DNA can be isolated from bacterial fractions containing 50 to 80% of the soil bacteria and may provide genetic information about the nonculturable bacteria in soil [6] ...on ...
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Combination of PURE DNA extraction and LAMP DNA amplification methods for accurate malaria diagnosis on dried blood spots
... LAMP (Kawai, unpublished data). The LAMP technique has been adapted for use in the diagnosis of different infectious diseases with notori- ously high burdens, including tuberculosis and malaria, for which high ...
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Detection of Chlamydia trachomatis DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems
... of DNA on sensitivity of detection limits of ...PBMO DNA In routine diagnostic settings, it might become necessary to postpone analysis or reevaluate previously evaluated samples of SF DNA in order ...
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Rapid Diagnosis of Extrapulmonary Tuberculosis by PCR: Impact of Sample Preparation and DNA Extraction
... of DNA for the efficiency of Roche Cobas Amplicor PCR for the rapid diagnosis of extrapulmonary ...for amplification performed on CTAB-Roche-extracted specimens confirms its superiority over ...
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Improved Amplification of Microbial DNA from Blood Cultures by Removal of the PCR Inhibitor Sodium Polyanetholesulfonate
... with PCR is failed amplification due to the presence of PCR ...the DNA, and was identified as sodium polyanetholesulfonate (SPS), a common additive to blood culture ...Like DNA, SPS is ...
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Forensic DNA collection: extraction of molecular information from buccal cells using direct amplification
... and extraction methods ...for DNA Analysis Methods” issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM) ...forensic DNA analysis ...varying PCR parameters utilized ...
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Amplification/PCR. Amplification Software 303
... real-time PCR, and a bright red formulation, easily visible when recovering reaction products (not recommended for sealing Microseal ® 384-well PCR ...
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Detection of bovine trichomoniasis with a specific DNA probe and PCR amplification system
... Smegma samples collected from healthy bulls that were determined to be uninfected on the basis of at least three consecutive negative culture samples were used as diluent in sensitivity.[r] ...
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