DNA extraction and PCR analysis
Methods for microbial DNA extraction from soil for PCR amplification
... co- extraction of inhibitors (Tables 2 and 3) and the greater confidence that bead beating would lyse all microbial cells in the soil, this was the method of choice and concentrated on for further analysis ...
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Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR
... gene PCR with analysis of cloned products to identify the contaminating species ...Statistical analysis. Each sample of extracted DNA was subjected to qPCR in duplicate, and extractions were ...
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Modified DNA Extraction for Rapid PCR Detection of Methicillin-Resistant Staphylococci
... normal PCR. DNA extraction for PCR performance was then modified by omission of achromopeptadiase and proteinase K digestion, phenol/chloroform extraction and ethanol ...positive ...
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Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification
... fly; DNA extraction; Calcium; PCR, lysis buffer, Lutzomyia ...the extraction of DNA from phlebotomines, including methods that eliminate DNA-associated proteins by using ...
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Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens
... final DNA yield. Purity of extracted DNA is of importance for down- stream molecular work and researchers often prefer to use kits that ensure removal of ...using DNA extracted by the four kits ...
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Evaluation of DNA Extraction and PCR Methods for Detection of Enterocytozoon bienuesi in Stool Specimens
... Inhibitors or other debris are effectively removed by washing reagent. Since the application of samples onto the FTA filters is very simple, a large number of samples for field epidemio- logical studies can be prepared ...
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Rapid DNA extraction and PCR validation for direct detection of Listeria monocytogenes in raw milk
... chemical analysis of raw milk simples For a microbial hazard, temperature is the major critical measure in several steps in dairy production; for this reason milk samples were aseptically taken and transported to ...
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Protocol optimization for genomic DNA extraction and RAPD-PCR in mosquito larvae (Diptera: Culicidae)
... ABSTRACT PCR based molecular markers are powerful tools for the analysis of genetic diversity for which isolation of good quality genomic DNA is ...three DNA extraction methods for 1, 5 ...
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Simple and quick method of DNA extraction from different parts of plant for PCR amplification
... the DNA molecular marker based genetic ...genomic DNA is the prerequisite for molecular biology research. An efficient DNA extraction method should be simple, rapid and affordable to provide ...
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FEASIBILITY OF CONDUCTING PCR-BASED DNA ANALYSIS AT THE CRIME SCENE
... uncommon occurrence. The determination of STR patterns in the field may protect the chain of custody of evidence since the analysts will have control of the samples. The entire protocol from extraction through STR ...
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A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods
... genomic DNA extraction from filamentous fungi with high-throughput ...alcohol extraction and precipitation with ...the DNA. Being a closed system of gDNA extraction, our method has been ...
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Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real Time PCR Assay
... (HCMV) DNA in blood is becoming the standard method for monitoring HCMV infection in immune-suppressed and unsuppressed ...HCMV DNA measured in whole blood (WB), peripheral blood leukocytes (PBL), and ...
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Significant impact of amount of PCR input templates on various PCR-based DNA methylation analysis and countermeasure
... unmethylated DNA substances are not available at present, we have to use the genomic DNA from cancer cell lines as the homogenously methylated and unmethylated reference DNA to obtain standard curves ...
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Comparison of DNA-extraction Methods Suitable for PCR-based Applications to Identify Shrimp Species in Commercial Products
... of DNA extraction were performed and compared in order to have the better DNA quality and quantity from specific seafood matrix such as various types of shrimp ...acids extraction efficiency ...
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Interlaboratory Evaluation of Different Extraction and Real Time PCR Methods for Detection of Coxiella burnetii DNA in Serum
... different PCR assays, with one major exception: the PCR that was in use in laboratory G was much less sensitive than the other PCR ...This PCR performed similarly to the other assays on a ...
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COMPARATIVE ANALYSIS OF HUMAN DNA EXTRACTION METHODS AND MITOCHONDRIAL DNA HV1 AND HV2 HAPLOGROUP DETERMINATION
... same extraction method, obtained concentrations were also higher (5-173 ...of DNA that can be a result of using ammonium acetate to eliminate ...for DNA extraction from many different sample ...
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Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
... the DNA extrac- tion quality of two systems, a semi-automatic and a manual DNA extraction system for the purpose of downstream PCR-DGGE ...of DNA is neces- sary. Based on the results of ...
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Extraction of DNA Extraction of DNA
... of DNA • The DNA can be stained by the inclusion of ethidium bromide in the gel, or by soaking the gel in a solution of ethidium bromide after ...The DNA shows up as an orange band on illumination by ...
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quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476
... ® PCR procedure for the determination of the presence of sequence-containing DNA in Rice species DNA extracted from Rice grain ...single DNA extraction is performed followed by two ...
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