DNA extraction methods
Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR
... Candida DNA were not detected in extrac- tion controls consisting of sterile water processed through each of the six DNA extraction methods compared in this ...fungal DNA when using a ...
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Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize
... of DNA [5, 7]. Several methods to isolate DNA from plant tissues are available; however, these methods produce either small amounts or DNA of inconsistent ...the DNA ...
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Evaluation of Automated and Manual Commercial DNA Extraction Methods for Recovery of Brucella DNA from Suspensions and Spiked Swabs
... commercial DNA extraction ...the DNA extraction methods required re- agents or equipment uncommon in clinical and diagnostic lab- ...the DNA extraction methods ...
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Detection of Chlamydia trachomatis DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems
... of DNA on sensitivity of detection limits of ...PBMO DNA In routine diagnostic settings, it might become necessary to postpone analysis or reevaluate previously evaluated samples of SF DNA in order ...
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Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food
... the DNA extraction methods in the A260/A280 absorbance ratio, an ANOVA in combination with Tukey test was performed independently for each group ...differences. DNA quality and purity was ...
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Comparison of Different DNA Extraction Methods for Forensic Samples
... Abstract DNA analyses are important for different types of research in biomedical and forensic science and DNA isolation is an often required ...different DNA isolation kits are constantly becoming ...
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Sample Collection and DNA Extraction Methods for Environmental DNA Metabarcoding in Headwater Streams
... 13 Gels were made with ~125 ml of 1% agarose stained with 2 μl of Ethidium bromide. All of the PCR reaction was loaded into gel wells with 4 μl of loading dye. Gel electrophoresis was conducted at 100 Volts and 400 mAmps ...
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Comparison of dna extraction methods for detection of citrus huanglongbing in colombia
... Capture of Diaphorina citri psyllids Adult individuals were collected using entomological nets, while the nymphs of D. citri were directly taken from symptomatic trees in different phenological stages (vege- tative, ...
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COMPARATIVE ANALYSIS OF HUMAN DNA EXTRACTION METHODS AND MITOCHONDRIAL DNA HV1 AND HV2 HAPLOGROUP DETERMINATION
... Mitochondrial DNA (mtDNA) is widely used in forensic analysis, human ancestry and genealogy, population genetics, evolutionary and molecular systematics ...nuclear DNA, mtDNA has a few advantages in this ...
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Evaluation of Two DNA Extraction Methods for Detection of Strongyloides stercoralis Infection
... combined methods is interesting. Both extraction methods in combination with day 1 and day 2 Baermann testing show a range of sensitivities, suggesting a variety in day-to-day larva ...molecular ...
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Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels
... environmental DNA (eDNA), originating from tissue, cells, or extracellular DNA, offers the opportunity to survey the biological composition of communities across multiple trophic levels from a non-invasive ...
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Qualification Study of Two Genomic DNA Extraction Methods in Different Clinical Samples
... of DNA extraction techniques by quantity (measurement of concentration of DNA extracted) and quality (260/280 ratio and PCR product) ...gDNA extraction techniques (a traditional salting-out ...
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Comparison of Total DNA Extraction Methods for Microbial Community Form Polluted Soil
... Abstract DNA isolation represents the basic and probably the most important step in molecular biology for microbial strains, and even more, for microbial community ...for DNA microbial community isolation, ...
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Comparison of Three Different DNA Extraction Methods for Linguatula serrata as a Food Born Pathogen
... The DNA samples in both groups were precipitated, using 50 µl of 3 M sodium acetate (NaAc 300 mM, pH ...each DNA pellets was washed with cold (- 20 °C) 70% ethanol (250 ...the DNA pellet was dried. ...
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Evaluation of DNA Extraction Methods on Individual Helminth Egg and Larval Stages for Whole-Genome Sequencing
... A key challenge of working with environmental stages of helminth parasites is that they are often immature, for example, eggs or early stage larvae, and extremely small (for example, Haemonchus contortus eggs are ...
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Methods for microbial DNA extraction from soil for PCR amplification
... of DNA-based techniques can overcome this limitation by allowing the fate of particular genes or organisms to be monitored directly in environmental ...extract DNA from soil and sediment initially used ...
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Human skeletal remains: development of DNA extraction and typing methods
... for DNA extraction from bones, verifying which kind of bone yields the best quality of DNA and evaluating different characteristics such as preservation, place of conservation and age of skeletal ...
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Evaluation of DNA Extraction and PCR Methods for Detection of Enterocytozoon bienuesi in Stool Specimens
... The DNA pellet was resuspended in 200 l of TE buffer and kept at ⫺20°C until ...three DNA extraction methods, a stool specimen with a known concentration of microsporidial spores was ...
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Combination of PURE DNA extraction and LAMP DNA amplification methods for accurate malaria diagnosis on dried blood spots
... samples were missed by the RDT while PURE–LAMP missed 1 P. falciparum and 1 P. ovale samples only. Discussion This study examined the sensitivity of PURE–LAMP with archived clinical blood samples stored on dried fil- ter ...
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Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
... bacterial DNA from specimens for molecular diagnostics, since fecal samples also contain DNA from human cells and many different substances derived from food, cell residues and medication that can inhibit ...
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