DNA extraction, PCR, cloning and sequencing
Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad range PCR, cloning and sequencing
... Broad-range PCR, sequencing and cloning are essential tech- niques for the characterization of the microbial environment in ...rDNA PCR coupled to automated DNA extraction to ...
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Detection and Identification of Mycobacterium tuberculosis in Joint Biopsy Specimens by rpoB PCR Cloning and Sequencing
... However, the differential diagnosis of M. tuberculosis arthritis and rheumatoid arthritis is problematic, especially in patients with joint biopsy samples that have nonspecific results by his- tological evaluation and ...
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Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58
... cell DNA and deep sequencing (RCA-seq) and by long PCR template cloning and ...template cloning and sequencing in decoding HPV58 ...long PCR template cloning and ...
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CHAPTER 6. CLONING AND SEQUENCING OF CCCVd-RELATED RT-PCR PRODUCTS
... The occurrence of these variants agrees with the RPA analysis (Chapter 4), which showed that the CCCVd-like RNAs in this palm had several mismatches when compared with CCCVd. The presence of sequences equivalent to OP ...
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Five-minute cloning of Taq polymerase-amplified PCR products for sequencing
... (ds) DNA containing a 5´ overhang or blunt ends to create single-stranded (ss) ...a DNA restriction fragment. After digestion of the DNA with exonuclease III, mung bean nuclease is used to remove all ...
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Characterization of a circulating PRRSV strain by means of random PCR cloning and full genome sequencing
... sequence-independent PCR techniques for the detection and characterization of PRRSV could be useful to bypass problems associated with the heterogeneity of this ...
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Broad range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian patients with reactive and undifferentiated arthritis
... bacterial DNA were mainly derived from commensals that are normally present in the skin and ...detected DNA of specific bacterial groups that have not been detected in arthritis samples or in human ...
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ECKER LAB PROTOCOL. Nested PCR and Sequencing of T-DNA Junctions in Arabidopsis
... Nested PCR and Sequencing of T-DNA Junctions in Arabidopsis Plant Growth Conditions The germination and DNA extraction of individual lines are done in 96-deep-well plates (Doc Frugal ...
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Determining of DNA-barcoding of 6 – 8 species by partial sequencing of mtDNA (CO1) using molecular method (PCR-Sequencing)
... Salari Aliabadi, M.A., Rezvani Gilklaei, S. Savari, A., Zolgharnain, H., Nabavi, S.M.B. 2008. Microsatellite polymorphism in Iranian population of Cobia, Rachycentron canadum . Biotechnology Sambrook J, Russell D. ...
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PCR Cloning Combined With DNA Barcoding Enables Partial Identification of Fish Species in a Mixed-Species Product
... for DNA sequencing. Prior to sequencing, each plasmid clone underwent a restriction digest that included 10 µl plasmid, ...that PCR inserts were still ...have PCR inserts, additional ...
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DNA Sequencing and PCR Markets
... http://www.researchandmarkets.com/reports/41864/ DNA Sequencing and PCR Markets Description: DNA sequencing and polymerase chain reaction (PCR) are two major technology platforms ...
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PCR & DNA Sequencing. PCR= Polymerase Chain Reaction. PCR applications
... of PCR reaction: • As a rule of thumb, use an annealing temperature (Ta) about 5 o C below the lowest Tm of the pair of primers to be used if a good yield of product is ...
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Hibbett lab protocols for DNA isolation, PCR, and DNA sequencing.
... of DNA on a sequencing ...the DNA polymerase requires the terminal OH group to incorporate the next ...new DNA fragment in the current cycle of a sequencing ...plasmid DNA, etc.) ...
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Investigating a Eukaryotic Genome: Cloning and Sequencing a Fragment of Yeast DNA
... of DNA molecules from the external ...the DNA entering the cell is called ...plasmid DNA to replicate while killing the bacteria that failed to take up a ...
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Comparison of two methods of generating random DNA fragments for cloning and sequencing
... nebulized DNA was repaired using T4 pohnucleotide kinase only or both T4 polynucleotide kinase and the Klenow ...for sequencing, and two separate runs on the ABI 370A DNA Sequencer were ...
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Mitochondrial DNA PCR and Sequencing. Table of Contents Fall 2012
... Lambda PCR, Alu PV92 PCR, PCR Optimization, D1S80 PCR, and Mitochondrial PCR Kits includes limited, non-transferable rights under the running-royalty component to use only this amount ...
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Cloning, sequencing, and functional analysis of a Marek's disease virus origin of DNA replication.
... Neither the replication origins of Epstein-Barr virus (oriP and oriLyt) nor those of cytomegalovirus contained sequences similar to the putative origin of serotype 2 MDV replication (1, [r] ...
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Methods for microbial DNA extraction from soil for PCR amplification
... of DNA-based techniques can overcome this limitation by allowing the fate of particular genes or organisms to be monitored directly in environmental ...extract DNA from soil and sediment initially used ...
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Optimized Extraction of Insect Genomic DNA for Long-Read Sequencing
... genomic DNA, but we found that grinding in liquid nitrogen and transfer resulted in lower yields, and sometimes lower ...genomic DNA to avoid shearing; if wide-bore tips are not available, use a sterile ...
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Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR
... gene PCR with analysis of cloned products to identify the contaminating species ...extracted DNA was subjected to qPCR in duplicate, and extractions were performed in duplicate or ...fungal DNA ...
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