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DNA Helicase

The Papillomavirus E1 Protein Forms a DNA-Dependent Hexameric Complex with ATPase and DNA Helicase Activities

The Papillomavirus E1 Protein Forms a DNA-Dependent Hexameric Complex with ATPase and DNA Helicase Activities

... has DNA helicase ...a DNA helicase assay, we analyzed the material from the gradient peak in an oligonucleotide displace- ment assay, as described by Seo and Hurwitz ...

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Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein

Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein

... and DNA helicase ...the helicase sub- strate ...HCV helicase strongly prefers poly(U) for the stim- ulation of ATPase activity (8, 34, ...HCV helicase by using a poly(U) affinity ...

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Bipartite Structure of the SGS1 DNA Helicase in Saccharomyces cerevisiae

Bipartite Structure of the SGS1 DNA Helicase in Saccharomyces cerevisiae

... a DNA helicase with homology to the human BLM and WRN ...conserved DNA helicase domain homologous to Escherichia coli RecQ and a large N-terminal domain of unknown ...and ...

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Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication.

Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication.

... 66, 1992 ROLE OF T ANTIGEN AS A REPLICATIVE DNA HELICASE the viral origin of DNA replication, a bilobed T-antigen helicase complex was observed operating simultaneously at both forks of [r] ...

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Biochemical Characterization of Adeno-Associated Virus Rep68 DNA Helicase and ATPase Activities

Biochemical Characterization of Adeno-Associated Virus Rep68 DNA Helicase and ATPase Activities

... both DNA helicase activity and terminal resolution site (trs) nicking ...contrast, DNA helicase activity was linear with re- spect to enzyme ...the DNA in the 3 * -to-5 * direction. ...

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Vaccinia virus gene A18R encodes an essential DNA helicase.

Vaccinia virus gene A18R encodes an essential DNA helicase.

... a DNA-dependent ATPase that contains the canonical sequence motifs associated with the DEXH group of DNA and RNA ...mammalian DNA helicase ...a DNA helicase that unwinds duplex ...

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Characterization of Plasmodium falciparum ATP dependent DNA helicase RuvB3

Characterization of Plasmodium falciparum ATP dependent DNA helicase RuvB3

... higher helicase activity than PfRuvB2 alone ...stronger DNA helicase activity than the individual pro- teins ...lacks helicase function ...appreciable helicase activity or that to ...

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Involvement of a replicative DNA helicase of bacteriophage T4 in DNA recombination.

Involvement of a replicative DNA helicase of bacteriophage T4 in DNA recombination.

... Accordingly, a null mutation in gene 61 may allow all gp41 to act in recombination, resulting in more frequent recom- bination and restricting recombination-dependent[r] ...

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Analysis of mitotic and meiotic defects in Saccharomyces cerevisiae SRS2 DNA helicase mutants.

Analysis of mitotic and meiotic defects in Saccharomyces cerevisiae SRS2 DNA helicase mutants.

... The SRS2 helicase is required for full meiotic viability: Mutations in several genes involved in mi- totic DNA repair and recombination have been found to limit the comp[r] ...

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Structural Basis of Bloom Syndrome (BS) Causing Mutations in the BLM Helicase Domain

Structural Basis of Bloom Syndrome (BS) Causing Mutations in the BLM Helicase Domain

... DEAH helicase or RecQCt ...DEAH helicase do- ...that helicase domain may retain its DNA helicase activity when the C-terminus is trun- cated downstream of the helicase domain of ...

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Remodeling of the Rad51 DNA Strand-Exchange Protein by the Srs2 Helicase

Remodeling of the Rad51 DNA Strand-Exchange Protein by the Srs2 Helicase

... Several DNA helicases (including the highly conserved Srs2 in Saccharomyces cerevisiae) downre- gulate Rad51 ...UvrD helicase (Marini and Krejci ...a DNA helicase domain that con- tains an ...

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Mutations in Sensor 1 and Walker B in the Bovine Papillomavirus E1 Initiator Protein Mimic the Nucleotide-Bound State

Mutations in Sensor 1 and Walker B in the Bovine Papillomavirus E1 Initiator Protein Mimic the Nucleotide-Bound State

... from DNA viruses belong to the superfamily 3 (SF3) helicases (5, ...papillomavirus DNA replication, has multiple functions that are affected by binding or hydrolysis of ATP (14, 18, 21, 23, 24, ...a ...

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Fusion of piggyBac-like transposons and herpesviruses occurs frequently in teleosts

Fusion of piggyBac-like transposons and herpesviruses occurs frequently in teleosts

... polymerase, DNA helicase, primase, ATPase sub- unit of terminase, major capsid protein, membrane glycoprotein, capsid triplex protein, capsid maturation protease, ORF34, ORF37, ORF44, ORF54, ORF56, ORF60, ...

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An in Trans Interaction at the Interface of the Helicase and Primase Domains of the Hexameric Gene 4 Protein of Bacteriophage T7 Modulates Their Activities

An in Trans Interaction at the Interface of the Helicase and Primase Domains of the Hexameric Gene 4 Protein of Bacteriophage T7 Modulates Their Activities

... with DNA helicase has emerged as an important element in the coordination of leading and lagging strand DNA synthesis at the replication fork (7, 34, ...of helicase and primase as individual ...

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Vaccinia virion protein I8R has both DNA and RNA helicase activities: implications for vaccinia virus transcription.

Vaccinia virion protein I8R has both DNA and RNA helicase activities: implications for vaccinia virus transcription.

... Vaccinia virions contain a fully functional transcription sys- tem (23). By using purified virion proteins and plasmid tem- plates containing vaccinia virus early promoters, early viral transcription can be demonstrated ...

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Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures

Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures

... the DNA annealing activity, which is not detected when the hairpin is present (Table ...impair DNA helicase activity but have little impact on the annealing activity, suggesting that other structural ...

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Identification of a Functionally Relevant Adeno-Associated Virus Rep68 Oligomeric Interface

Identification of a Functionally Relevant Adeno-Associated Virus Rep68 Oligomeric Interface

... RBS-containing DNA did not appear to be com- promised by this ...RBS-mediated DNA binding but fails to promote the subsequent DNA nicking ...origin DNA to allow the trs nicking reaction to ...

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Defects in DNA Lesion Bypass Lead to Spontaneous Chromosomal Rearrangements and Increased Cell Death

Defects in DNA Lesion Bypass Lead to Spontaneous Chromosomal Rearrangements and Increased Cell Death

... Translesion DNA synthesis suppresses GCR accumulation in mph1 ⌬ ...translesion DNA syn- thesis (41). To determine how spontaneous DNA lesions are processed if they cannot be bypassed by Mph1 or TLS, ...

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Structure-Based Mutational Analysis of the Bovine Papillomavirus E1 Helicase Domain Identifies Residues Involved in the Nonspecific DNA Binding Activity Required for Double Trimer Formation

Structure-Based Mutational Analysis of the Bovine Papillomavirus E1 Helicase Domain Identifies Residues Involved in the Nonspecific DNA Binding Activity Required for Double Trimer Formation

... and DNA helicase activity the ␤ -hairpin is clearly involved in both these ...nonspecific DNA binding had helicase defects, we tested the mutants for helicase activity in an oligo- ...

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DNA induces conformational changes in a recombinant human minichromosome maintenance complex

DNA induces conformational changes in a recombinant human minichromosome maintenance complex

... ATP-dependent DNA unwinding activity has been demon- strated for recombinant archaeal homohexameric minichromo- some maintenance (MCM) complexes and their yeast hetero- hexameric counterparts, but in higher ...

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