Dot blot
Rapid and Accurate Genotyping of YMDD Motif Variants in the Hepatitis B Virus Genome by an Improved Reverse Dot Blot Method
... The reverse dot blot (RDB) method can be used to detect many mutants simultaneously. Samples differing only in one nucleotide are easily distinguished (5, 19). We combined “flow- through hybridization” ...
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Development of Dot-blot Hybridization Based on 522 bp Repetitive Sequence (R522) for Detection of Toxoplasma gondii
... In order to take suitable measures and to prevent disease outbreaks, an early diagnosis of toxoplasmosis is essential and the availability of efficient and sensitive diagnostic tools is of great importance, particularly ...
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Simple and Sensitive Detection of HBsAg by Using a Quantum Dots Nanobeads Based Dot-Blot Immunoassay
... a dot blot immunoassay was conducted with HBsAg proteins dispersed in 1% healthy human ...the dot-blot immunoassay can be used to detect relevant samples in a complex protein ...
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<p>Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species</p>
... the dot- blot ELISA ...of dot signal intensity for each dot selected was acquired by selecting Analysis, Gel, Plot ...the dot signal intensity was delineated using the straight line ...
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DNA amplification and reverse dot blot hybridization for detection and identification of mycobacteria to the species level in the clinical laboratory
... DNA Amplification and Reverse Dot Blot Hybridization for Detection and Identification of Mycobacteria to the Species Level in the Clinical Laboratory ELLEN H.. BROOKS' Microbiology Secti[r] ...
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Use of a Reverse Dot Blot Procedure To Identify the Presence of Multiple Serovars in Chlamydia trachomatis Urogenital Infection
... We are studying chlamydia transmission between sexual contacts and reinfection patterns in a large group of adolescent women where unambiguous identification of serotypes and detection of mixed infection are essential. ...
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<p>Evaluation of PCR-Reverse Dot Blot Human Papillomavirus Genotyping Test in Predicting Residual/Recurrent CIN 2+ in Posttreatment Patients in China</p>
... PCR-reverse dot blot (PCR-RDB) Yaneng ® Human Papillomavirus Genotyping Kit (Yaneng Biotech, Shenzhen, China), which detects 18 HR-HPV types (HPV-16, − 18, − 31, − 33, − 35, − 39, − 45, − 51, − 52, − 53, − ...
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Original Article Reverse dot blot hybridization for detection of gyrA mutation for rapid diagnosis of Mycobacterium tuberculosis resistant to fluoroquinolones
... Abstract: Rapid detection and timely treatment are of great significance to reduce Mycobacterium tuberculosis (M. tuberculosis) transmission, especially in cases suffering from the infection of multidrug-resistant (MDR) ...
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Analysis of differentially expressed genes in two immunologically distinct strains of Eimeria maxima using suppression subtractive hybridization and dot blot hybridization
... Real-time validation of the genes identified by SSH To verify that genes identified by SSH combined with dot-blot hybridization were differentially expressed in the SH and NT strains, real-time PCR was ...
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Specific detection and typing of adenovirus types 40 and 41 in stool specimens by dot blot hybridization
... A dot-blot hybridization test was developed which allowed the direct detection of fastidious enteric adenovirus DNA in stool specimens from children with diarrhea and simultaneous typing[r] ...
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Reverse Dot Blot Assay (Insertion Site Typing) for Precise Detection of Sites of IS6110 Insertion in the Mycobacterium tuberculosis Genome
... Reverse dot blot hybridization assays involve the amplifica- tion and labeling of the DNA sequence of interest (the target DNA), followed by hybridization of the labeled amplicon to oligonucleotides (the ...
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Dot blot hybridization assay of B19 virus DNA in clinical specimens
... A total of 81 specimens gave reactions in the dot blot hybridization assay that were considered weakly positive, i.e., equal to or less than the reaction given by the 100-pg B19 DNA cont[r] ...
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Problems in detection of cytomegalovirus in urine samples by dot blot hybridization
... Hybridization with both probes and conventional methods virus isolation and detection of early nuclear protein yielded concordant results with 98 samples; 83 samples were negative and 15[r] ...
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Detection of enterotoxigenic Escherichia coli by dot blot hybridization with biotinylated DNA probes
... Three biotin-labeled DNA Bio-DNA probes corresponding to structural genes specifying heat-labile and heat-stable enterotoxins of porcine and human origin were prepared by random priming;[r] ...
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Dot blot hybridization assay for chicken anemia agent using a cloned DNA probe
... Additional experiments indicated that the DNAs extracted from MDCC-MSB1 cells infected with the Cux-1, Gifu-1, EF88/78/276, 87/10/44, and 89/3713 isolates of CAA produced positive hybrid[r] ...
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A Simple Method for Detection of Multiple Chemical Specific IgGs in Serum Based on Dot Blotting
... by dot blot assay using X-HSA ad- ducts as the ...by dot blot assays and that chemical-HSA adducts generated under alkaline conditions could be used as ...
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I., S LOVÁ EK J., Z EMÁNKOVÁ M. (2002): Utilisation of immu- nochemical methods for detection of Colletotrichum spp. in strawberry . Plant Protect. Sci., 38 : 55–63.
... Based on the results we summarise that a latent infec- tion of strawberry by C. acutatum can be detected using antibody K 91. After artificial infection of roots we ob- served gradual presence of the pathogen in roots ...
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Tweet
... slot dot-blot method is superior, as it allows for more of the an- tigen of interest to be loaded on a carrier ...slot dot-blot method allowed us, with satisfying reproducibility, to detect ...
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Identification of the lytic origin of DNA replication in human cytomegalovirus by a novel approach utilizing ganciclovir-induced chain termination.
... For quantitation of this type of analysis, we studied the total intracellular viral DNA from DHPG-treated cultures by using dot blot hybridization, and we compared the levels of accumula[r] ...
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DIFFERENT TYPES OF ANTIOXIDANT ACTIVITY OF AERVA PERSICA (BURM F ) MERR
... like; dot blot DPPH method, DPPH scavenging activity, ABTS method, reducing power assay, OH radical scavenging assay, nitric oxide scavenging assay, total antioxidant activity (Phosphomolybdenum (PMo) ...
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