Double Stranded RNA Synthesis
Rotavirus Replication: Plus-Sense Templates for Double-Stranded RNA Synthesis Are Made in Viroplasms
... We show here that rotavirus gene-specific siRNAs induced formation of silencing complexes in infected cells that caused near-complete reductions in the expression of the nonessential protein NSP1, the viroplasm protein ...
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Primer-Dependent and Primer-Independent Initiation of Double Stranded RNA Synthesis by Purified Arabidopsis RNA-Dependent RNA Polymerases RDR2 and RDR6
... single stranded RNAs (ssRNAs) produced by the plant-specific RNA polymerase ...aberrant RNA transgenes during post-transcriptional gene silencing (PTGS) [ 11 ], tasiRNAs [ 12 – 15 ] and natsiRNA [ 16 ...
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Effect of Viral Double-Stranded RNA on Protein Synthesis in Intact Cells
... The addition of purified, noninfectious, double-stranded RNA of bovine en- terovirus, a picornavirus, to intact cells in culture results in a rapid cessation of cellular polypeptide synt[r] ...
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Synthesis of Double-Stranded RNA in a Virus-Enriched Fraction from Agaricus bisporus
... Electron micrograph of the 35-nm spherical virus particles (a>) which banded with the peak of RNA polymerase activity after Cs2SO4 isopycnic centrifugation. Centrifugation and prepara[r] ...
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Guanidine Hydrochloride Inhibits Mammalian Orthoreovirus Growth by Reversibly Blocking the Synthesis of Double-Stranded RNA
... and RNA replication and provides a powerful tool to synchronize the initiation of negative-sense RNA synthesis during in vitro replication ...a double-stranded RNA (dsRNA) virus ...
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Double-Stranded RNA Binding by Human Cytomegalovirus pTRS1
... We investigated the hypothesis that the products of these HCMV genes act by binding to dsRNA. pTRS1 expressed by cell-free translation or by infection of mammalian cells with HCMV or recombinant VV bound to dsRNA. ...
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Template Role of Double-Stranded RNA in Tombusvirus Replication
... Positive-stranded RNA viruses of plants use their RNAs as the templates for ...plus-strand synthesis. To characterize the nature of the (ⴚ)RNA in the membrane-bound viral replicase, we ...
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Microglia Recognize Double-Stranded RNA via TLR3
... Total RNA was then isolated using the TRIzol reagent according to the manufacturer’s instruction (Invitrogen Life ...Purified RNA was digested with DNase I to remove trace contaminating genomic DNA using ...
Dependence of minus-strand synthesis on complete genomic packaging in the double-stranded RNA bacteriophage phi 6.
... form dsRNA, and then transcribes dsRNA to form plus-strand message. The minus-strand synthesis of a particular genomic segment is dependent on prior packagng of the other segments. The S[r] ...
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Noncytopathic Bovine Viral Diarrhea Virus Inhibits Double-Stranded RNA-Induced Apoptosis and Interferon Synthesis
... NTPase, RNA helicase, and protease activities of this nonstructural protein (26, 27) appear to be preserved in the cp biotype of ...viral RNA present in cells infected by cp BVDV may vastly exceed that in ...
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Evidence that vesicular stomatitis virus produces double-stranded RNA that inhibits protein synthesis in a reticulocyte lysate.
... Comparative electrophoretic analysis of endogenous proteins synthesized by nuclease-treated reticulocyte lysates in the presence of various K+ concentrations and poly(A)+ RNA (0.5 ,ug/ml[r] ...
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Nonspecific Nucleoside Triphosphatase P4 of Double-Stranded RNA Bacteriophage φ6 Is Required for Single-Stranded RNA Packaging and Transcription
... minus-strand synthesis ac- tivities to the wt ...plus-strand synthesis reaction conditions with and without RNA addition and analyzed the reactions in sucrose gradients ...without RNA ...
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Comparison of Polymerase Subunits from Double-Stranded RNA Bacteriophages
... phage RNA metabolism through selective initiation at the transcriptional promoter, the question arises whether a similar regulatory function could also be assigned to the polymerases of 8 and ...catalyze ...
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Molecular details of the double-stranded RNA virus replication and assembly
... Since, RNA synthesis requires entry of the 3’-end of the positive-strand to the template tunnel, the binding of the polymerase to the 5’-UTR may indicate that this region assists the co-assembly of hPBV ...
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Genome organization of RNA tumor viruses. I. In vitro synthesis of full-genome-length single-stranded and double-stranded viral DNA transcripts.
... stranded DNA on alkaline sucrose gradients. The long M-MLV were analyzed on alkaline sucrose gradients. Labeled cDNA transcrpts were then used as templates polyoma DNA forn II was includ[r] ...
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Visualization of Double-Stranded RNA in Cells Supporting Hepatitis C Virus RNA Replication
... viral RNA and may act to regulate the switch between RNA repli- cation and virion assembly (12, 20, 41, ...The RNA foci associated with NS5A on the ER membrane presumably re- flected sites of viral ...
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RNA-Binding Activity of the Rotavirus Phosphoprotein NSP5 Includes Affinity for Double-Stranded RNA
... the synthesis of the seg- mented double-stranded RNA (dsRNA) rotavirus ...proteins. RNA-binding assays demonstrated that NSP5 has unique nonspecific RNA-binding activity, ...
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Semiconservative synthesis of single-stranded RNA by bacteriophage phi 6 RNA polymerase.
... The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bac- teriophage 46 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome b[r] ...
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A deletion mutant of L-A double-stranded RNA replicates like M1 double-stranded RNA.
... Ethidium bromide-stained agarose gel (A) and autoradio- gram (B) of the dsRNAs contained in purified VLPs from strain RE455 by cesium chloride density gradient centrifugation and the pro[r] ...
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Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase
... To further define the domains responsible for binding to dsRNA, we cloned the mouse dsRNA-activated p65 kinase and used sequence align- ment to identify conserved domains in the N-termin[r] ...
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