EBNA-1
Dominant-negative inhibitors of EBNA-1 of Epstein-Barr virus.
... of EBNA-1 are likely to inhibit wild-type EBNA-1 when they occupy only a subset of the binding sites on ...for EBNA-1 are present in the FR of oriP, approximately six to seven ...
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A subpopulation of normal B cells latently infected with Epstein-Barr virus resembles Burkitt lymphoma cells in expressing EBNA-1 but not EBNA-2 or LMP1.
... with EBNA-2 to -6, according to two different viral programs, activated or silenced, depending on the cell pheno- ...express EBNA-1 from a promoter designated Fp but not EBNA-2 to ...
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The Epstein-Barr virus EBNA-1 promoter Qp requires an initiator-like element.
... 2 1 (QRE-2) was targeted by mutation, con- sistent with the result obtained previously in EBV-positive BL cells ...(to 1 75) and distal (to 1 5068) sequence ...to 1 5068. Be- cause the ...
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Repression of Epstein-Barr Virus EBNA-1 Gene Transcription by pRb during Restricted Latency
... of EBNA-1 in the maintenance (if not replication) of the EBV genome dictates that EBNA-1 be expressed in a proliferating cell (3), why would there be a need to repress EBNA-1 in ...
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Functional domains of Epstein-Barr virus nuclear antigen EBNA-1.
... To identify the EBNA-1 nuclear localization signal, small oligonucleotides representing EBNA-1 sequences that encode clusters of basic peptides were transferred into two different vector[r] ...
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Mapping EBNA-1 Domains Involved in Binding to Metaphase Chromosomes
... EGFP EBNA-1 are specifically associated with metaphase ...that EBNA associates with human and mouse chromo- somes during ...EBV EBNA-1 was cloned in frame with the 39 end of the ...
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Analysis of EBNA 1 and LMP 1 variants in diseases associated with EBV infection in Chinese children
... the EBNA-1 variations have focused on nasopharyngeal carcinoma ...V-val EBNA-1 with the functional advan- tage compared with prototype shown in their study might contribute to the ...
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Levels of Epstein-Barr virus DNA in lymphoblastoid cell lines are correlated with frequencies of spontaneous lytic growth but not with levels of expression of EBNA-1, EBNA-2, or latent membrane protein.
... 1 0022-538X/90/010437-08$02.00/0 Copyright ©D 1990, American Society for Microbiology Levels of Epstein-Barr Virus DNA in Lymphoblastoid Cell Lines Are Correlated with Frequencies of Spo[r] ...
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Expression of Epstein-Barr Virus Nuclear Antigen 1 Is Associated with Enhanced Expression of CD25 in the Hodgkin Cell Line L428
... Table 1 summarizes the results of the surface immunofluorescence analyses of five representative EBNA-1-expressing and vector control-fluorescein isothio- cyanate (FITC)-phycoerythrin (PE), ...
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Interaction of Epstein-Barr virus nuclear antigen 1 with the viral latent origin of replication.
... The presence of single and double point mutations in EBNA-1-binding site 2 did not alter the ability of EBNA-1 to induce KMnO4 reactivity at nucleotides 9046/9047 and 9110/9111, nor were[r] ...
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EBNA-2 and EBNA-3C extensively and mutually exclusively associate with RBPJkappa in Epstein-Barr virus-transformed B lymphocytes.
... in 1 ml of lysis buffer (1% Nonidet P-40, 50 mM Tris-HCl [pH ...fluoride, 1 mM sodium or- thovanadate) for 30 min at ...rabbit EBNA-2 (E2) antibody, 50 m l of monoclonal D8 (EBNA-3C ...
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Interaction of Epstein-Barr Virus Nuclear Antigen Leader Protein (EBNA-LP) with HS1-Associated Protein X-1: Implication of Cytoplasmic Function of EBNA-LP
... LMP1, EBNA-2, EBNA-3A, and EBNA-3C have been shown to be essential for EBV-induced immortalization (7, 12, 19, ...26). EBNA-2 con- tributes to B-cell immortalization by regulating EBV latency ...
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Variable expression of latent membrane protein in nasopharyngeal carcinoma can be related to methylation status of the Epstein-Barr virus BNLF-1 5'-flanking region.
... We have investigated Seven virus-coded proteins are regularly expressed in Epstein-Barr virus EBV transformed lymphoblastoid cell lines LCLs: the nuclear proteins EBNA-1 to EBNA-6 and th[r] ...
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Autorepression of Epstein-Barr Virus Nuclear Antigen 1 Expression by Inhibition of Pre-mRNA Processing
... of EBNA-1 to inhibit splicing and possibly other pre-mRNA processing appears to be strictly dependent on the context of the EBNA-1 binding sites within the gene ...which EBNA-1 ...
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Maintenance of Serum Immunoglobulin G Antibodies to Epstein-Barr Virus (EBV) Nuclear Antigen 2 in Healthy Individuals from Different Age Groups in a Japanese Population with a High Childhood Incidence of Asymptomatic Primary EBV Infection
... and 1 (EBNA-2 and EBNA-1, respectively) were studied using sera from healthy individuals of a population with a high incidence of asymptomatic primary EBV infections during infancy or ...
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Residues 231 to 280 of the Epstein-Barr Virus Nuclear Protein 2 Are Not Essential for Primary B-Lymphocyte Growth Transformation
... of EBNA-2 is not ...of EBNA-2 to transactivate gene ...assays, EBNA-2 deleted for codons 231 to 280 accumulated to higher levels and was similar to wild-type EBNA-2 in activation of the BamC ...
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Identification of Major Phosphorylation Sites of Epstein-Barr Virus Nuclear Antigen Leader Protein (EBNA-LP): Ability of EBNA-LP To Induce Latent Membrane Protein 1 Cooperatively with EBNA-2 Is Regulated by Phosphorylation
... antigen 1 [EBNA-1], 2, 3A, 3B, 3C, and leader protein [LP] and latent membrane protein 1 [LMP-1], 2A, and 2B) (17, 30), although the EBV genome encodes more than 80 ...on ...
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Rep*: a Viral Element That Can Partially Replace the Origin of Plasmid DNA Synthesis of Epstein-Barr Virus
... 1925 and 1926, the two plasmids behave similarly. In fact, during the time course of this experiment, the absolute amount of replicated 1925 and 1926 would have increased more than 2,500-fold, were all progeny cells to ...
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Use of enzyme linked immunosorbent assays with chimeric fusion proteins to titrate antibodies against Epstein Barr virus nuclear antigen 1
... In this work, we established an indirect ELISA and a sandwich ELISA to titrate antibodies against EBNA-1 with chimeric EBNA-1 fusion proteins and compared the titers of anti-EBNA antibod[r] ...
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Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B, and EBNA-3C genes.
... We determined the DNA sequence of the AG876 EBV-2 EBNA-3 coding region and have compared it with known B95-8 EBV-1 EBNA-3 sequences to delineate the extent of divergence between EBV-1 an[r] ...
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