Embryonic and Germ Stem Cells
VSELs may obviate cryobanking of gonadal tissue in cancer patients for fertility preservation
... OSE cells smears showed the presence of small (2–5μm), round cells which expressed pluripotent markers SSEA- 4, Oct-4, Nanog, Sox-2, and c-kit [5, 6, ...of stem cells in OSE smears including ...
8
STELLA Facilitates Differentiation of Germ Cell and Endodermal Lineages of Human Embryonic Stem Cells
... ES cells over-expressing STELLA cDNA encoding the human STELLA homologue was amplified using One-Step RT-PCR kit ...ES cells were dissociated using 0.05 % trypsin. One million human ES cells were ...
8
piRNAs and PIWI proteins: regulators of gene expression in development and stem cells
... the germ plasm – a specialized cytoplasm required for germ cell ...the germ plasm ...to embryonic patterning (Rouget et ...in embryonic development through the regulation of essential ...
13
A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions
... labeled cells formed multiple organs in chimeric mice suggesting that, when placed in the proper conditions, the cells could differentiate into multiple different lineages ...+ stem cells ...
10
Differentiation of Human Embryonic Stem Cells into Embryoid Bodies Comprising the Three Embryonic Germ Layers
... ES cells grown on mouse embryo fibroblasts (as feeder ...hematopoietic cells (18; ...ES cells grown on feeders, as demonstrated by low levels of -fetoprotein and -globin in the ES ...
8
Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns
... into germ cell lineages, specifi cally in vitro-derived PGCs (invPGCs) ...into germ cells by forming embryoid bodies (EBs) with retinoic acid and culturing these EBs for 28 ...28, germ ...
14
AKT signaling promotes derivation of embryonic germ cells from primordial germ cells
... various stem cell ...pluripotent cells from spermatogonial stem cells is enhanced by the p53 deficiency (Kanatsu-Shinohara et ...for germ cells to acquire ...ES cells ...
11
EXAMINATION OF THE GERM CELL CHIMERA FORMING POTENTIAL OF MOUSE EMBRYONIC STEM CELLS
... mouse embryonic stem cells (ES ...derived cells could contribute to the germ ...aneuploid cells, in R1 ES cell line, dramatically increased after 10 ...ES cells contain ...
6
Fish Stem Cell Cultures
... Stem cells have the potential for self-renewal and differentiation. First stem cell cultures were derived 30 years ago from early developing mouse ...pluripotent embryonic stem (ES) ...
11
Impaired meiotic competence in putative primordial germ cells produced from mouse embryonic stem cells
... somatic cells in the male gonadal ridge degrades ...the embryonic ovary allows PGCs to enter meiosis (for a review, see Bowles and Koopman, ...that germ cell sex depends on sex-determining signalling ...
9
Making gametes from alternate sources of stem cells: past, present and future
... tent stem cells termed very small embryonic-like stem cells (VSELs) in all adult organs including testis and ovary, which can also be differentiated into gametes in ...into germ ...
14
Targeted DamID reveals differential binding of mammalian pluripotency factors
... homogeneous cells and antisera with high ...fewer cells than ChIP-based ...mouse embryonic stem cells, epiblast-like cells and primordial germ cell-like cells ...
11
Retinoic acid improve germ cell differentiation from human embryonic stem cells
... spermatogonial stem cells (SSCs). These cells are able to undergo meiosis, generate haploid male gametes, and may fertilize after intracytoplasmic injection into mouse ...oocyte-like cells. ...
8
Stem Cell Diversity and Applications in Therapy
... Embryonic stem cells develop from the inner cell mass (ICM) of an early embryo that is in the blastocyst ...These cells undergo the process of differentiation to form the primitive ectoderm ...
11
Current protocols in the generation of pluripotent stem cells: theoretical, methodological and clinical considerations
... Pluripotent stem cells have been derived from various embryonic, fetal and adult ...sources. Embryonic stem cells (ESCs) and parthenogenic ESCs (pESCs) are derived from the ...
15
A germ cell origin of embryonic stem cells?
... ES cells most closely represent early germ cells is that the very earliest events of ES cell differentiation into somatic and extra- embryonic lineages will not accurately reflect events that ...
7
Embryonic and Adult Stem Cells
... Stem cells are currently only being used to a limited extent in routine clinical ...that stem cells can be utilized in the treatment of a wide array of human disorders by replenishing lost or ...
5
Understanding Regenerative Medicine and Its Position in Healthcare
... of stem cells within intravenous infusion (Silva & Lederman, ...Surgical stem cell implantation is a highly targeted method of delivery that would take place in high demanding circumstances so ...
12
Age-old wisdom concerning cell-based therapies with added knowledge in the stem cell era: our perspectives
... of cells, there is no single active ingredi- ent for which an appropriate dose can be ...of cells may differ markedly, and it is near impossible to create a physiological environment in vitro which mimics ...
6
Stem cells: past, present, and future
... including stem cells [151], and are involved in intercellular communication through the delivery of their mRNAs, lipids, and ...prove, stem cells, together with their paracrine fac- ...
22