Enhancer element
Induction of chronic human immunodeficiency virus infection is blocked in vitro by a methylphosphonate oligodeoxynucleoside targeted to a U3 enhancer element.
... One such oligomer, targeted to an NF-KB enhancer element, inhibited phorbol myristate acetate induction of viral replication and tat-mediated trans activation of the human immunodeficien[r] ...
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The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene.
... for enhancer activity, am- plified viral DNA fragments were purified through agarose gels, digested with BamHI, and inserted into the BglII site of the enhancerless reporter plasmid pCAT-promoter (Promega, ...
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A new enhancer element, ENII, identified in the X gene of hepatitis B virus.
... It functioned much better in the highly differentiated hepatoma cells but not in nonliver cells or 3977 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A new enhancer e[r] ...
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The state of cellular differentiation determines the activity of the adenovirus E1A enhancer element: evidence for negative regulation of enhancer function.
... Infection of undifferentiated rodent cells with a mutant adenovirus that lacked the DNA sequences necessary for 4AP3 binding generated enhancer activity, thus demonstrating that this fac[r] ...
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EBNA1 can link the enhancer element to the initiator element of the Epstein-Barr virus plasmid origin of DNA replication.
... EBNA1 protein bound to a plasmid containing FR, the herpes simplex virus type 1 thymidine kinase promoter, and the firefly luciferase gene was electroporated into cells.. Downloaded from[r] ...
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Identification of a novel constitutive enhancer element and an associated binding protein: implications for human papillomavirus type 11 enhancer regulation.
... To investigate possible interactions among the full-length HPV-11 E2 protein and cellular factors that recognize CEI, CEII, and the E6 TATA motif, the same series of CAT expression clone[r] ...
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Molecular dissection of segment formation in the developing hindbrain
... initiator enhancer element to shape krox20 initial transcriptional response: Hox PG1 and Nlz factors define the anterior-posterior extent of the enhancer ’ s domain of activity, whereas Fgf ...
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Alternate promoter selection within a human cytomegalovirus immediate-early and early transcription unit (UL119-115) defines true late transcripts containing open reading frames for putative viral glycoproteins.
... Temporal patterns of human cytomegalovirus transcription: mapping the viral RNAs synthesized at immediate early, early, and late times after infection.. An enhancer element in the short [r] ...
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The l(3)S12 locus of Drosophila melanogaster: heterochromatic position effects and stage-specific misexpression of the gene in P element transposons.
... The sensitivity of certain 1(?)S12+ insertions to site-specific euchromatic position effect appears to be due to separation of the gene from an endogenous enhancer element [r] ...
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Human cytomegalovirus ie1 transactivates the alpha promoter-enhancer via an 18-base-pair repeat element.
... The target for human CMV iel transactivation, the 18-bp repeat element, has limited similarity to a core enhancer element from SV40 and is very similar to the NKKB transcription factor-b[r] ...
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The hepatitis B virus enhancer modulates transcription of the hepatitis B virus surface antigen gene from an internal location.
... Insertion of the enhancer element HpaI-HpaII 5' to the pre-Sl promoter element plasmid pENAP resulted in no increase in HBsAg gene expression Fig.. However, insertion of the enhancer bet[r] ...
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Regulation of the human papillomavirus type 11 E6 promoter by viral and host transcription factors in primary human keratinocytes.
... Contrary to previous studies with immortalized human cervical carcinoma C-33A cells, constitutive enhancer element II in the upstream regulatory region conferred no enhancer activity and[r] ...
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Tandemly Integrated HPV16 Can Form a Brd4 Dependent Super Enhancer Like Element That Drives Transcription of Viral Oncogenes
... FIG 5 Brd4 is enriched at the integrated HPV16 genome in 20861 cells. Chromatin immunoprecipitation (ChIP) was performed in 20861 and 20863 cells using antibodies against Brd4, H3K27ac, and MED1. ChIP DNA samples were ...
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piggyBac-Based Insertional Mutagenesis and Enhancer Detection as a Tool for Functional Insect Genomics
... and enhancer activities should be detected simultaneously and ...vivo enhancer detection in tissues such as the eyes and central nervous system and other tissues in which the artificial 3xP3 promoter drives ...
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Looking beyond GWAS: allele-specific transcription factor binding drives the association of GALNT2 to HDL-C plasma levels
... Background: Plasma levels of high-density lipoprotein cholesterol (HDL-C) have been associated to cardiovascular disease. The high heritability of HDL-C plasma levels has been an incentive for several genome wide ...
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Cooperative activation of cardiac transcription through myocardin bridging of paired MEF2 sites
... Fig. 4. Bridging of MEF2C-bound MEF2 sites by myocardin-935. (A) Schematic of the in vitro pull-down assay. (B) qPCR detection of co-precipitated MEF2 site 1 after incubation with biotinylated MEF2 site 2 in the presence ...
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A Gata2 intronic enhancer confers its pan endothelia specific regulation
... We previously reported that a 271 kbp Gata2 yeast artificial chromosome (YAC) transgene, containing sequences from –198 to +73 kbp (with respect to the translation initiation site) of the Gata2 locus was capable of ...
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Enhancer traps in the Drosophila bithorax complex mark parasegmental domains.
... Competition of regulatory signals: Several enhancer trap lines carry regulatory DNA fragments from the bithorax complex within the P element, and these se- quences d[r] ...
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Genetic variation in T box binding element functionally affects SCN5A/SCN10A enhancer
... We provide the first functional evidence to our knowledge for the direct involvement of a human SNP in cardiac conduction system gene regulation. This SNP was found to be associated with increased PR/QRS duration, which ...
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Interactions of the transcription factors MIBP1 and RFX1 with the EP element of the hepatitis B virus enhancer.
... EP element is located in the HBV enhancer region, which is activated in hepatic cells, we compared the migrations of DNA-protein complexes of EP and MIF-1 sites with nuclear extracts derived from the HepG2 ...
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