Epstein-Barr nuclear antigen-1
Optimal Transactivation by Epstein-Barr Nuclear Antigen 1 Requires the UR1 and ATH1 Domains
... Epstein-Barr nuclear antigen 1 (EBNA1) is essential for Epstein-Barr virus to immortalize naïve B ...region 1 (LR1), has been identified previously as being ...
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Epstein Barr Nuclear Antigen 1 modulates replication of oriP plasmids by impeding replication and transcription fork migration through the family of repeats
... Plasmids AGP73, and AGP74 have been described previ- ously [24], and contain 10 and 20 EBNA1-binding sites in the FR respectively. These plasmids are constructed in the backbone of pPUR, and also contain EBV's DS and the ...
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Autorepression of Epstein-Barr Virus Nuclear Antigen 1 Expression by Inhibition of Pre-mRNA Processing
... Here we show that EBNA-1 posttranscriptionally regulates expression from its promoter Qp by inhibiting pre-mRNA pro- cessing. Further, by detection of unspliced transcripts originat- ing from Qp during latency ...
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Epstein-Barr Virus Nuclear Antigen 1 Hijacks the Host Kinase CK2 To Disrupt PML Nuclear Bodies
... the nuclear localization of the ⌬ 387-394 and ⌬ 395-450 EBNA1 mutants that fail to bind CK2 and USP7, respectively, soon after their expression when PML NBs are largely ...
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Environmental and genetic factors in pediatric inflammatory demyelinating diseases
... controls, Epstein-Barr virus (EBV) nuclear antigen-1 seropositivity was significantly associated with an increased risk for MS after adjusting for age, sex, race, ethnicity, and ...
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EBNA1 distorts oriP, the Epstein-Barr virus latent replication origin.
... * 1786 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The Epstein-Barr virus nuclear antigen 1 EBNA1 protein binds and activates the latent replication origin oriP of [r] ...
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Identification of candidate genes with pro-apoptotic properties by functional screening of randomly fragmented C DNA libraries
... cdna libraries and then treated simultaneously with both the agonistic anti-cd95 antibody cH11 and hy- gromycin. after four weeks, surviving clones stably expressing antisense cdnas were picked manually to purify the ...
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Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.
... 1-8 Copyright C 1977 American Society for Microbiology Solubilization ofthe Epstein-Barr Virus-Determined Nuclear Antigen and Its Characterization as a DNA-Binding Protein JANoS LUKA,* W[r] ...
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Epstein-Barr virus nuclear antigen 3C is a transcriptional regulator.
... Since EBNA-3C increases expression of LMP-1, we exam- ined the ability of EBNA-3C to activate expression from the LMP-1 promoter in transient transfection assays. EBNA-3C increased expression from the ...
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Characteristics of viral protein expression by Epstein-Barr virus-infected B cells in peripheral blood of patients with infectious mononucleosis.
... of Epstein-Barr virus (EBV) antigen-positive B cells in the peripheral blood of patients with infectious mononucleosis compared with that for latently EBV-infected individuals was examined by immu- ...
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Epstein-Barr Virus Nuclear Antigen 3C and Prothymosin Alpha Interact with the p300 Transcriptional Coactivator at the CH1 and CH3/HAT Domains and Cooperate in Regulation of Transcription and Histone Acetylation
... Cell lines. Burkitt’s lymphoma-derived cell line BJAB was obtained from Elliott Kieff and was cultured in RPMI 1640 medium (Gibco-BRL, Gaithersburg, Md.) supplemented with 5 g of penicillin/ml, 5 U of streptomycin/ml, ...
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Interaction of Epstein-Barr virus nuclear antigen 1 with the viral latent origin of replication.
... The presence of single and double point mutations in EBNA-1-binding site 2 did not alter the ability of EBNA-1 to induce KMnO4 reactivity at nucleotides 9046/9047 and 9110/9111, nor were[r] ...
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Human regulatory proteins associate with non coding RNAs from the EBV IR1 region
... the Epstein–Barr nuclear antigen leader protein (EBNA-LP), and occurs during cancer- associated latency III infection as well as in a rare latency program found in Burkitts lymphomas: ...
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Salivary and serum IL-10, TNF-α, TGF-β, VEGF levels in oropharyngeal squamous cell carcinoma and correlation with HPV and EBV infections
... of Epstein-Barr nuclear antigen 2 ...for 1 min, 55 °C for 1 min, 72 °C for 2 min and the final extension at 72 °C for 5 ...with 1 μl of first round PCR prod- uct in 30 ...
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Nuclear Import of Epstein-Barr Virus Nuclear Antigen 1 Mediated by NPI-1 (Importin α5) Is Up- and Down-Regulated by Phosphorylation of the Nuclear Localization Signal for Which Lys379 and Arg380 Are Essential
... of nuclear import efficiency of EBNA-1 is involved in its multifunctional properties, we first performed DNA replication/retention assays by cotransfection of plasmid pME18-EBNA1 with oriP-BamHI C-Luc or ...
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Functional domains of Epstein-Barr virus nuclear antigen EBNA-1.
... To identify the EBNA-1 nuclear localization signal, small oligonucleotides representing EBNA-1 sequences that encode clusters of basic peptides were transferred into two different vector[r] ...
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Conserved Region CR2 of Epstein-Barr Virus Nuclear Antigen Leader Protein Is a Multifunctional Domain That Mediates Self-Association as well as Nuclear Localization and Nuclear Matrix Association
... Plasmids. To construct pAS-EBNA-LPR1 and pAS-EBNA-LPR4, EcoRI and PstI fragments of pGBT9-EBNA-LPR1 and pGBT9-EBNA-LPR4 (19), respec- tively, were cloned into EcoRI and PstI sites of pAS2-1 (Clontech, Palo Alto, ...
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Antibody to the Rheumatoid Arthritis Nuclear Antigen: ITS RELATIONSHIP TO IN VIVO EPSTEIN BARR VIRUS INFECTION
... nuclear antigen was present in 26 (50%) of ...arthritis nuclear antigen, only 2 were positive during the 1 ...to Epstein-Barr nuclear antigen, another ...
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Epstein-Barr virus-specific intrathecal oligoclonal IgG production in relapsing-remitting multiple sclerosis is limited to a subset of patients and is composed of low-affinity antibodies
... for nuclear viral proteins released from dying cells ...continual antigen stimulation without a significant production of specific CSF and serum anti- bodies ...
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Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer
... for 1 min, 55 °C for 1 min, 72 °C for 2 min and the final extension at 72 °C for 5 ...with 1 μl of first round PCR product in 30 cy- cles with an annealing temperature at 60 ...Table 1 ...
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