ethyl esterification matrix-assisted laser desorption/ionization time-of-flight mass sectrometry
Mass determination of major plasma proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
... of matrix to protein to provide for efficient ionization of undiluted ...specimens. Matrix usually needs to be in more than 1000-fold molar excess relative to protein to yield good spectra ...of ...
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii
... LT mass spectrometer (Bruker Daltonics), and spectra were recorded in the positive linear mode (laser frequency, 20 Hz; ion source 1 voltage, 20 kV; ion source 2 voltage, ...average mass, the average ...
5
Successful Identification of Clinical Dermatophyte and Neoscytalidium Species by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... Reference database. The database for dermatophyte and Neoscyta- lidium identification was engineered using a previously described strategy (8, 12). A set of reference strains selected as belonging to clinically relevant ...
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Evaluation of the Andromas Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Aerobically Growing Gram Positive Bacilli
... the mass spectrum of the tested isolate with the spectra included in the Andromas database, taking into account a possible MS peak variation of ⫾ 10 m/z ...
6
Comparison of the Microflex LT and Vitek MS Systems for Routine Identification of Bacteria by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... three matrix-assisted laser desorption ionization–time of flight mass spectrometry sys- tems: Microflex LT (Bruker Daltonics, Bremen, Germany), Vitek MS RUO (Axima ...
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Rapid Identification of Positive Blood Cultures by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using Prewarmed Agar Plates
... sufficient time would be included in the next scheduled MALDI-TOF MS ...the time to the identification of pathogens and resistance, the next challenge will be working with clinicians to have these rapid ...
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Evaluation of blood culture broths with lysis buffer to directly identify specific pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry methods
... The mass spectra show the difference in peaks (presence or absence) and their intensities between the sample spectrum and those of bacteria identified at first pass by ...
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Evaluation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Nontuberculous Mycobacteria from Clinical Isolates
... In summary, MALDI-TOF identification of NTM species proved decisively superior to GenoType CM/AS both in accuracy (94.4% versus 84.0% according to the genomic sequencing) and in turnaround time (90 min versus 8 ...
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Comparison of analytical profile index, microscan walkaway 40 plus and bruker maldi biotyper systems for identification of enteric pathogens in Kenya
... Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) also known as Bruker MALDI Biotyper is a technology that allows instant ...
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A retrospective study of the incidence, clinical characteristics, identification, and antimicrobial susceptibility of bacteremic isolates of Acinetobacter ursingii
... one matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrom- eter, and the antimicrobial susceptibilities of the ...
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Original Article Weak cation exchange magnetic beads coupled with MALDI-TOF-MS in screening serum markers in perimenopausal women with osteopenia
... and matrix- assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) in screening serum protein markers of osteopenia from 10 ...
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Reduction of Internal Standard Signals in Quantitative MALDI TOF Mass Spectrometry
... of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to the quantitation of various ...
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Fatal Nocardia farcinica Bacteremia Diagnosed by Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass Spectrometry in a Patient with Myelodysplastic Syndrome Treated with Corticosteroids
... and time-consuming ...by Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass ...therapy. Matrix-Assisted Laser ...
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Performance of Two Resin Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems
... We compared the clinical performances of the BacT/Alert Plus (bioMérieux) and Bactec Plus (Becton Dickinson) aerobic and anaerobic blood culture (BC) media with adsorbent polymeric beads. Patients > 16 years old with ...
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Identification and Characterization of Clostridium difficile Sequence Type 37 Genotype by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... whole-cell mass spectra for ST37 and non-ST37 genotypes, using ATCC reference strains and several additional clinical isolates ...The mass spectra were acquired in the mass range of m/z 2,000 to ...
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Rapid Identification of Clinically Relevant Members of the Genus Exophiala by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Description of Two Novel Species, Exophiala campbellii and Exophiala lavatrina
... MS analyses of Exophiala isolates. Since it is well established that the performance of MALDI-TOF MS is highly dependent on spectral database inclusivity (20, 22), repre- sentative NCPF isolates corresponding to the 18 ...
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Identification of bacterial urinary tract pathogens from urine samples using conventional methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry
... 3.1 mass spectrometer, the growth of isolated colony from agar plate was taken and applied onto the Maldi ...of matrix solution (saturated solution of HCCA [α-cyano-4-hydroxy cinnamic acid] inorganic ...
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Identification of Mycobacteria by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
... in mass spectrometry (MS) have made it possible to use whole-cell matrix-assisted laser desorption ionization– time-of-flight MS (MALDI-TOF MS) or MALDI to aid in ...
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Microbial Typing by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?
... ties. Thus, it would be simplistic to attempt to superimpose PFGE interpretative guidelines on data generated by whole-cell MS. Nevertheless, from a purely numerical standpoint, PFGE and MS data sets are quite similar, ...
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Accuracy of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Clinical Pathogenic Fungi: a Meta Analysis
... Subanalysis of studies in which the clinical strains were supple- mented with reference strains decreased the heterogeneity to 66.4% with a 95% CI of 1.5% to 88.5% and improved the accuracy to 0.988 with a 95% CI of ...
10