Fast protein liquid chromatography
FAST PROTEIN LIQUID CHROMATOGRAPHY OF CAMEL α-LACTALBUMIN FRACTION WITH RADICAL SCAVENGING ACTIVITY
... by fast protein liquid ...cation-exchange chromatography is of great interest to yield, in a single step, whey protein fractions with various biological activities, ...
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Isolation of Potentially Useful Antigens from Cyathostomin Third-Stage Larvae by Using a Fast Protein Liquid Chromatography One-Step Method
... In contrast, metabolic products (excretory/secretory anti- gens) were collected by incubating L3 cyathostomins in RPMI liquid culture medium and used in the present work. Their application makes it possible to ...
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Purification And Characterization Of G. M. Morsitans Midgut Trypanolysin
... a fast protein liquid chromatography (FPLC) ...ion-exchange chromatography was loaded via a 500 μl loop into a Mono Q HR 5/5 an ion-exchange column (Pharmacia, Uppsala, Sweeden) ...
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Apolipoprotein E in diabetic dyslipidemia and atherosclerosis
... by fast protein liquid chromatography (FPLC), over 60% of the plasma cholesterol was in the LDL fraction in Ldlr -/- Apobec1 -/- mice with wild type ...
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Heparin binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin 6 by porcine endometrial and cervical cells and bovine endometrial cells
... HiPrep 16/10 Heparin FF, 20 ml (GE Healthcare Bio- Sciences AB, Uppsala, Sweden) by fast protein liquid chromatography (FPLC) according to Sanz, et al. [12] and Varilova, et al. [24] with some ...
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Clinical Decision Making of Nurses Regarding Elder Abuse
... transporter to obtain heme. SiaA (HtsA, spy1795), a lipoprotein located on the cell surface, serves as a heme binding protein. To understand the iron-uptake mechanism, histidine 229, one of the two proposed axial ...
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Comparative kinetic analysis of two fungal β-glucosidases
... Results: A comparative kinetic analysis of b -glucosidase from Aspergillus niger and BGL1 from Trichoderma reesei, purified using a new and efficient fast protein liquid chromatography ...
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Quantitative comparison of lipoprotein fractions derived from human plasma and serum by liquid chromatography-tandem mass spectrometry
... their protein constituents, which vary greatly among the different lipoprotein ...with fast protein liquid chromatography-size exclusion chromatography (FPLC-SEC) from both human ...
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Trimethylamine-N-oxide (TMAO)-induced atherosclerosis is associated with bile acid metabolism
... by fast-protein liquid chromatography (FPLC) with slight modification according to Sips et ...gel-filtration chromatography using Superdex 200 10/ 300 GL (GE Healthcare Bio-Sciences AB, ...
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An UFLC-DAD Method for the Quantification of Menaquinone-4 in Spiked Rabbit Plasma
... Ultra-Fast Liquid Chromatography-Diode Array Detector (UFLC-DAD) method for the quantification of vitamin K2 as Menaquinone-4 (MK-4) in rabbit ...each. Protein precipitation was carried out ...
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Proteomics analysis of faecal proteins in the tick Haemaphysalis flava
... the protein components in the faeces of the tick Haemaphysalis flava, by liquid chromatography – tandem mass spectrometry (LC/MS-MS) to identify potential protein antigens for vaccine ...
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FAST AND SENSITIVE VALIDATED METHOD FOR QUANTITATION OF HALOPERIDOL IN HUMAN PLASMA USING ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) WITH DIODE ARRAY DETECTION
... Performance Liquid Chromatography (HPLC) methods with UV detection [4,5] , caplillary gas-liquid chromatography [6] , coulometric [7,8] and fluorescence detection [9,10] were reported to ...
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ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-A NEW REVOLUTION IN LIQUID CHROMATOGRAPHY
... Performance Liquid Chromatography” or "Ultra High Pressure Liquid ...faster chromatography or a combination of both or Increase sensitivity, due to sharper (narrower) and higher ...
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DEVELOPMENT AND VALIDATION OF RAPID AND SENSITIVE HPLC METHOD FOR THE DETERMINATION OF 5-FLUOROURACIL IN HUMAN SERUM
... analyzing chromatography each standard solution, two standard curves were prepared by plotting the ratio of peak area of 5-fu to internal standard (thymine) versus concentration of ...
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Development and application of a UHPLC-MS/MS metabolomics based comprehensive systemic and tissue-specific screening method for inflammatory, oxidative and nitrosative stress
... The different recoveries of the ISTDs from the six tissues reflected the diverse nature of these tissues (Fig. 4, panel a, Table S6). It is important to note that the recoveries reported here do not assess the extent of ...
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Fast and Sensitive Chiral Analysis of Amphetamines and Cathinones in Equine Urine and Plasma Using Liquid Chromatography Tandem Mass Spectrometry
... gas chromatography (GC), capillary electrophoresis (CE), and high performance liquid chro- matography (HPLC) have been used for enantiomer analysis [5] [8] [9] ...[12]-[14]. Liquid chro- matography ...
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Overexpression, extraction, purification and characterisation of DppA from Escherichia coli
... A recombinant E. coli expression system was used to produce DppA in this study as the process has many advantages when compared to the other available systems including rapid growth, cheap cultivation, a well-studied ...
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HPLC and LCMS – A review and a recent update
... HPLC methods are historically divided into two different sub-classes based on stationary phases and the corresponding required polarity of the mobile phase. Use of octadecylsilyl (C18) and related organic-modified ...
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Quantitation of Sildenafil in Human Plasma by Reverse Phase High Performance Liquid Chromatography
... with liquid-liquid extraction was calculated by comparing the peak ...underwent liquid-liquid extraction to that of identical concentrations of sildenafil prepared in methanol without ...
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Development of Novel Liquid Chromatography-Electrospray Tandem Mass Spectrometry Approaches for the Structural Characterization of Brevetoxins Including in vitro Metabolites
... by liquid chromatography-negative ion electrospray-tandem mass spectrometry, with the approach of adding fluoride to promote [M + F] - adducts; good detection limits and linearity were ...
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