Flow-Cytometer
EVALUATION OF ANTIMICROBIAL STUDIES ON ETHANOLIC EXTRACTS AND POLY HERBAL FORMULATIONS OF ABUTILON INDICUM, ARISTOLOCHEA BRACTEOLATE, AND ANDROGRAPHIS PANICULATA BY USING FLOW CYTOMETER
... plots. Flow cytometer’s onboard software (Cell Quest Pro), was used to analyze the fluorescence dot plots and to determine the percentage of PI - (living) and PI + (dead) bacterial ...
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Evaluation of the Partec Flow Cytometer against the BD FACSCalibur System for Monitoring Immune Responses of Human Immunodeficiency Virus-Infected Patients in Zimbabwe
... Equipment used in the study. (i) Partec Cyflow SL_3. The Partec Cyflow SL_3 is a single-platform, three-parameter (SSC plus two-color fluorescence) desktop flow cytometer. It contains a solid-state laser ...
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Flow Cytometer Performance Characterization, Standardization and Calibration against CD4 on T Lymphocytes Enables Quantification of Biomarker Expressions for Immunological Applications
... Quantitative flow cytometry has been demonstrated in a single laboratory study ...various flow cytometer platforms have not yet been reported, suggesting the enormous difficulty of the ...all ...
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Automated bedside flow cytometer for mHLA-DR expression measurement: a comparison study with reference protocol
... and flow histograms, we noticed satisfactory results for a comparison ...no flow histogram available on Accellix, there is no interpretation of the results based on usual gating strategy or fluorescence ...
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Screening for Urinary Tract Infection with the Sysmex UF 1000i Urine Flow Cytometer
... urine flow cytometer able to detect particles in urine, including leukocytes and bacteria, quickly by staining the particles with fluorescent dyes and with subsequent identification by imped- ance, ...
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Evaluation of a method for counting absolute numbers of cells with a flow cytometer.
... measured 55 instead of 50 ml of whole blood, this could explain the increased values. The pipettes used in this study, however, were carefully calibrated by the manufacturer to deliver 50 ml. Another explanation might be ...
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P53 Expression in Acute Lymphblastic Leukemia in Sudanese Patients Using Flow Cytometer
... This study was conducted to detect of p53 expression in acute lymphocytic leukemia in Sudanese patients using the FlowCytometry and data were collected from the pa[r] ...
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Development of a helium neon laser based flow cytometer for evaluation of particulate matter
... SYSTEM DESCRIPTION Overview The most crucial part of the system is the flow chamber which is used to confine cells in the central part of the flow stream for exposure to the laser beam w[r] ...
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Original Article Effects of target regulation of LDHA through the PDK1/Akt/mTOR pathway on myocardial apoptosis caused by ischemia/reperfusion injury in rats
... group, flow cytometer to detect myocardial apoptosis, Western blot method to detect the effect of the LDHA overexpression on PDK1/AKt/mTOR protein expression un- der H/R and dual-luciferase method to ...
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Validation of a single platform, volumetric, flow cytometry for CD4 T cell count monitoring in therapeutic mobile unit
... Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) for CD4 T cell numeration in a mobile unit, compared against a reference flow cytometry ...FACSCalibur flow ...
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A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors
... but are generally expensive [13]. In this work, we propose to use a benchtop Accuri flow cytometer as the basis for the design of an automated FC. This apparatus was recently tested on microbiological ...
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Rapid production of antigen-specific monoclonal antibodies from a variety of animals
... tissue were suspended in 1 ml PBS-BSA and stained with fluorescently labeled antigen (0.1 µg/ml) and fluorescently labeled antibody against IgG at 4°C for 30 minutes with gentle agitation. After washing with PBS, the ...
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Rice bran derivatives alleviate microglia activation: possible involvement of MAPK pathway
... FACScaliburTM flow cytometer (BD Biosciences, San Jose, CA, USA) equipped with argon laser (488 ...the cytometer, dot plots were displayed to identify the cells based on their physical parameters on ...
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Using Fluorescent Microspheres as a Non-Biological Surrogate Indicator for Sequential Disinfection Performance.
... different flow cytometer and software was used in this study than what was reported in the ...Different flow cytometers have different relative fluorescence scales/FITC resolution ...the flow ...
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Use of Formalin-Fixed, Propidium Iodide-Stained Human Leukocytes as a Standard for Enumerating CD4+ T Lymphocytes in a Single-Platform Assay
... of flow cytometers with precision fluidics may represent the long-term solution to problems of interlaboratory variation, most laboratories still rely on flow cytometers that lack the ability to measure ...
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Reliable measurement of E. coli single cell fluorescence distribution using a standard microscope set-up
... a flow cyt- ometer, we validated the set-up described above compar- ing its results with those obtained with a flow cytometer, the gold standard for the acquisition of single-cell fluor- ...with ...
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ANTIPROLIFERATIVE AND APOPTOGENIC EFFICACY OF ANTIDIABETIC DRUGS METFORMIN AND SITAGLIPTIN AGAINST MCF7 AND HEPG2 CANCER CELLS: A COMPARATIVE MOLECULAR STUDY
... by flow cytometer demonstrated that only metformin but not sitagliptin could induce early apoptotic cell death in breast cancer cells but it could vastly induce the late apoptotic signs in MCF7 cells ...
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High-resolution underway measurements of phytoplankton photosynthesis and abundance as an innovative addition to water quality monitoring programs
... the flow cytometer data were significantly affected by en- vironmental ...the flow cytometer measuring the fluorescence per par- ticle, while the FRRf does a measurement of the bulk sam- ple, ...
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Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts
... the flow cell of a Luminex flow cytometer: For the Luminex system, a microsphere assay mix is made with the intent that equal numbers of each bead classifier be present in the ...the flow ...
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Stress Response in Candida albicans Induced by Boric Acid
... more surviving cell bodies (flow cytometer events) than in water. Water without boric acid showed higher counts and little loss of viability. Programmed cell death may occur through apoptosis or in some ...
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