fluorescence in situ hybridization
The Use of Restriction Fragment Length Polymorphism and Fluorescence in Situ Hybridization to Investigate Microbiota of Piglets after Feeding Oregano
... for fluorescence in situ hybridization (FISH) were immediately fixed in Carnoy’s solution [18] for at least 12 ...the hybridization step ...probes. Hybridization temperature was 50 ˚C ...
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Assessment of ERBB2 and EGFR gene amplification and protein expression in gastric carcinoma by immunohistochemistry and fluorescence in situ hybridization
... transmembrane segment (aa 633-654), and a cytoplas- mic domain that exhibits tyrosine kinase activity (aa 655-1234) [2,3], ERBB2 is frequently unregulated in human cancers such as breast cancer [4], ovarian cancer [5], ...
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Applications of Fluorescence in Situ Hybridization (FISH) for Detecting Genetic Changes in Hematological Malignancies
... in situ hybridization (FISH) has become an important tool both for defining initial chromosomal abnormalities within a disease process, and for monitoring response to therapy as well as minimal residual ...
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Array-based comparative genomic hybridization is more informative than conventional karyotyping and fluorescence in situ hybridization in the analysis of first-trimester spontaneous abortion
... as fluorescence in situ hybridization (FISH), quantitative fluorescence polymerase chain reaction (QF-PCR) and subtelomeric multiplex ligation-dependent probe ampli- fication, which do not ...
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Clinical significance of pancreatic circulating tumor cells using combined negative enrichment and immunostaining-fluorescence in situ hybridization
... Methods: In the present study, we applied a newly-developed platform integrated subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH) to analyze clinical ...
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Development of an Improved Method of Mitotic Metaphase Chromosome Preparation Compatible for Fluorescence in situ Hybridization in Cotton
... ly Fluorescence In situ Hybridization (FISH) and Genomic In situ Hybridization (GISH), are excellent tools to study the structure and func- tion of chromosomes, polyploidy, aneuploidy, ...
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A Molecular Cytogenetic Map of Sorghum Chromosome 1: Fluorescence in Situ Hybridization Analysis With Mapped Bacterial Artificial Chromosomes
... We used structural genomic resources for Sorghum bicolor (L.) Moench to target and develop multiple molecular cytogenetic probes that would provide extensive coverage for a specific chromosome of sorghum. Bacterial ...
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Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology
... tions, fluorescence in situ hybridization (FISH) is used in genetic toxicology for analysis of chromosome damage with increased efficiency and specificity for identifying certain kinds of chromosomal ...
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Rapid Diagnosis of Bacterial Meningitis by Real Time PCR and Fluorescence In Situ Hybridization
... and fluorescence in situ hybridization (FISH) were evaluated as rapid methods for the diagnosis of bacterial meningitis and compared to standard diagnostic ...
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Visualization of the impatiens downy mildew pathogen using fluorescence in situ hybridization (FISH)
... in situ hybridization (FISH) has been widely used as a cultivation-independent tool for direct detection, identification and quantification of microor- ganisms ...(3) hybridization of the probe; (4) ...
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Comparative Investigation of Conventional Cytogenetic and Fluorescence in situ Hybridization in Children with Acute Lymphoblastic Leukemia
... Article citation: Olga B, Minira B, Meryert K, Dina N, Kaliyash J, Anna M, Riza B. Comparative investigation of conventional cytogenetic and fluorescence in situ hybridization in children with ...
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Fluorescence in situ hybridization (FISH): an increasingly demanded tool for biomarker research and personalized medicine
... test, fluorescence in situ hybridization (FISH) does not show signs of fading; on the contrary, it plays an increasingly important role in detecting specific biomarkers in solid and hematologic ...
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Localization of Single- and Low-Copy Sequences on Tomato Synaptonemal Complex Spreads Using Fluorescence in Situ Hybridization (FISH)
... F OR most plant species, what is known about the Stack 1995). The most direct means of determining order of loci on chromosomes is based almost en- the location of genes on chromosomes is through the tirely on genetic ...
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Faster Identification of Pathogens in Positive Blood Cultures by Fluorescence In Situ Hybridization in Routine Practice
... a hybridization chamber in a water bath at 48°C for 90 ...After hybridization, unbound probe was washed ...by fluorescence microscopy (Olympus BX40; magnification, ⫻ 100) with a fluorescein ...
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Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes
... new fluorescence in situ hybridization (FISH) method with peptide nucleic acid (PNA) probes for identification of Staphylococcus aureus directly from positive blood culture bottles that contain ...
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Comparison of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assessment for Her 2 status in breast cancer
... (IHC), fluorescence in situ hybridization (FISH), chromogenic in situ hybridisation (CISH) ...expensive fluorescence microscope equipped with multi-band-pass fluorescence ...
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The detection of hTERC amplification using fluorescence in situ hybridization in the diagnosis and prognosis of cervical intraepithelial neoplasia: a case control study
... in situ, and 28 with invasive squamous carcinoma, as well as 25 patients who were at 2-year follow-up after either Loop Electrosurgical Excision treatment (n = 11) or radical surgery (n = ...interphase ...
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Original Article Myxoid and reticular angiomatoid fibrous histiocytoma: a case confirmed by fluorescence in situ hybridization analysis for EWSR1 rearrangement
... USA), CD68 (KP-1, Dako), CD99 (O13, Dako), glial fibrillary acidic protein (GFAP) (polyclonal, Dako), P63 (BC4A4, Dako), CD21 (1F8, Dako), CD35 (Ber-MAC-DRC, Dako), and Ki67 (MIB-1, Dako). Appropriate positive and ...
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Original Article Detection of cutaneous protothecosis by fluorescence in situ hybridization
... Abstract: Objective: To evaluate the value of fluorescence in situ hybridization (FISH) as a tool for the detection of cutaneous protothecosis. Methods: A mouse model of cutaneous protothecosis was ...
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<p>Identification of chromosomal abnormalities and genomic features in near-triploidy/tetraploidy-acute leukemia by fluorescence in situ hybridization</p>
... by fluorescence in situ hybridization with 19 commercially available probes that detected a small portion of gene alterations and large regions of chromosome ...Using fluorescence in ...
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