fluorescence lifetime (FLT)
A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein
... beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the ...fibre. Fluorescence lifetime responses were recorded ...
5
Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... light. Fluorescence emission in PDT may be used to monitor the uptake process but fluorescence intensity is subject to variability due to scattering and absorption; the addition of fluorescence ...
10
Single Cell Assay for Molecular Diagnostics and Medicine: Monitoring Intracellular Concentrations of Macromolecules by Two-photon Fluorescence Lifetime Imaging
... excited fluorescence lifetime imaging ...the fluorescence lifetime of fluorophore and the refractive index of its microen- vironment varying due to changes in the concentrations of ...
12
Time resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A 4 in solution and in cellular systems
... non-exponential fluorescence decays in some solvents that remains to be investigated further and is suggestive of excited charge transfer processes, the differences in lifetimes (Figure 4) and emission spectra ...
21
A CMOS time-resolved fluorescence lifetime analysis micro-system
... conventional fluorescence intensity measurements are prone to misinterpretation due to illumination and fluorophore concentration ...time-resolved fluorescence detection, whereby the characteristic ...
20
Single-shot time-gated fluorescence lifetime imaging using three-frame images
... the fluorescence lifetime images with a single excitation to freeze the instantaneous ...the fluorescence delay functions by gating the image intensifier of an ICCD camera with different ...
20
Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy
... Two-photon fluorescence lifetime imaging analysis of transfected insect ...Two-photon-induced fluorescence lifetime images were ob- tained with multiphoton microscopy apparatus and an Eclipse ...
10
Towards unsupervised fluorescence lifetime imaging using low dimensional variable projection
... The first example is to study the fluorescence lifetimes of the autofluorescence emitted from daisy pollen. Autofluorescence of biological samples as well as the fluorescence lifetime can be very ...
15
Ultra portable explosives sensor based on a CMOS fluorescence lifetime analysis micro system
... This work explores the use of a green-light-emitting copolymer as a chemosen- sor to detect nitroaromatic-based explosive vapors by recording photoluminescence (PL) and time-resolved PL decay. We show successful ...
11
Fluorescence lifetime imaging of E combretastatin uptake and distribution in live mammalian cells
... combretastatins, fluorescence lifetime imaging was used with two-photon excitation ...intrinsic fluorescence with quantum yields and lifetimes that depend markedly on solvent polarity and ...used ...
23
Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches
... Time-correlated single photon counting (TCSPC) systems are widely as the gold standard fluorescence lifetime imaging (FLIM) owing to high temporal resolution. The acquisition speed has been significantly ...
5
Using in vivo fluorescence lifetime imaging to detect HER2-positive tumors
... the fluorescence lifetime of tumor and contralateral sites were measured in ...measured fluorescence intensity at the tumor and contra-lateral sites at different time points for 5 ...
6
Direct Vpr Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging
... The fluorescence lifetime images were recorded and compared with those of the two wild type Vpr fusion ...the lifetime images of the Vpr-eGFP mutants expressed in the absence (Column A) and in the ...
17
HYDROXYPROPYL β -CYCLODEXTRIN INCLUSION COMPLEX OF CURCUMIN USING FLUORESCENCE LIFETIME MEASUREMENT.
... state fluorescence measurements, there are not many reports on the fluorescence lifetimes of ...the fluorescence lifetimes considerably. The fluorescence decay profiles, obtained from time ...
7
GPU acceleration of time-domain fluorescence lifetime imaging
... Abstract. Fluorescence lifetime imaging microscopy (FLIM) plays a significant role in biological sciences, chem- istry, and medical ...up lifetime calculations compared to CPU-OpenMP (parallel ...
11
Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays
... high-speed lifetime imaging is lifetime ...the lifetime calculations while maintaining enough imaging ...full fluorescence emission histogram is needed for detailed examinations, it will take ...
20
Theoretical Studies of Single Molecule Biophysical Systems and Photochemical Ensembles
... In relation to the single molecule enzyme experimental observations on the fluctuations of catalysis rate, spectral diffusion and fluorescence lifetime, a formulation based on fluctuatio[r] ...
155
Homomultimerization of the Coxsackievirus 2B Protein in Living Cells Visualized by Fluorescence Resonance Energy Transfer Microscopy
... The 2B protein of enteroviruses is the viral membrane-active protein that is responsible for the modifications in host cell membrane permeability that take place in enterovirus-infected cells. The 2B protein shows ...
11
Synthesis, characterization and evaluation of aza dipyrromethenes and other small molecules for organic photovoltaics : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University, Te Kunenga ki Pūrehuroa, University of New Zealand, Palmerston North, New Zealand
... Figure 3.52 Fluorescence lifetime decay of compound 125 in the absence of PC60BM black line and the presence of PC60BM colored lines..... Figure 3.53 Fluorescence lifetime decay of compo[r] ...
367
Estimation of fluorescence lifetimes via rotational invariance techniques
... new fluorescence lifetime estimation via rotational invariance techniques (FLERIT) were developed for multiexponential fluorescence lifetime imaging (FLIM) ...
9