fluorescence lifetime imaging microscopy
Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... steady-state fluorescence intensity still suffered from artifacts arising from the heterogeneity of tissue optical properties [8], local environment, unknown chromophores with over- lapped spectra, and the ...
10
Mapping the lignin distribution in pretreated sugarcane bagasse by confocal and fluorescence lifetime imaging microscopy
... scanning microscopy (CLSM) and fluorescence lifetime imaging microscopy (FLIM) are able to provide important information about the concen- tration and the spatial distribution of a ...
10
Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy
... laser microscopy studies showed that enhanced green fluorescent protein (EGFP)-tagged nucleocapsids and capsid proteins aligned and colocalized with the peripheral microtubules of virus-infected insect ...with ...
10
Signal peptide peptidase (SPP) dimer formation as assessed by fluorescence lifetime imaging microscopy (FLIM) in intact cells
... Results: Fluorescence lifetime imaging microscopy (FLIM) can be is used to determine intra- or intermolecular interactions by fluorescently labeling epitopes on one or two different ...
8
Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells
... FLIM: fluorescence lifetime imaging microscopy; GCTOFMS: gas chromatography time-of-flight mass spectrometry; GSH: reduced glutathione; GSSG: glutathione disulfide, oxidized glutathione; ...
17
A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein
... beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the ...fibre. Fluorescence lifetime responses ...
5
Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches
... non-iterative fluorescence lifetime imaging (FLIM) analysis method was verified in an FPGA ...Keywords: Fluorescence lifetime imaging microscopy (FLIM), Time-correlated ...
5
HIV-1 Vpr Oligomerization but Not That of Gag Directs the Interaction between Vpr and Gag
... developed fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer-based assays to monitor the interaction between Pr55 Gag and Vpr in HeLa ...
12
Novel Bioluminescent Activatable Reporter for Src Tyrosine Kinase Activity in Living Mice
... Molecular imaging, especially optical imag- ing, provides a new platform for noninvasive visual- ization of biological processes at the molecular level in ...Optical imaging, including fluorescence ...
16
Micro or nanorod and nanosphere structures derived from a series of phenyl porphyrins
... force microscopy (AFM) studies were performed using an Explorer ...AFM. Fluorescence microscopy studies were performed using a Zeiss inverted microscope system coupled with a 532 nm excitation ...
8
GPU acceleration of time-domain fluorescence lifetime imaging
... Abstract. Fluorescence lifetime imaging microscopy (FLIM) plays a significant role in biological sciences, chem- istry, and medical ...up lifetime calculations compared to CPU-OpenMP ...
11
Direct Vpr Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging
... Co-localization of Vpr-eGFP and either mCherry-Vpr or Vpr-mCherry was visualized by confocal microscopy. As a control, Vpr-eGFP was first co-expressed with mCherry. Localization of Vpr-eGFP at the nuclear rim ...
17
Fluorescence lifetime imaging of E combretastatin uptake and distribution in live mammalian cells
... for imaging with fluorescence ...UV fluorescence in the region of 340-400 nm from intracellular 5-hyroxytryptophan, serotonin and propranolol using 2PE at 630 ...of fluorescence intensity (I) ...
23
Luminescence lifetime imaging microscopy by confocal pinhole shifting (LLIM-CPS)
... Initially, fluorescence of TMRM increased slightly after 2 min, perhaps due to continued loading into mitochondria (Figure ...TMRM fluorescence was lost, indicating depolarization of ...
125
Correlated fluorescence quenching and topographic mapping of Light-Harvesting Complex II within surface-assembled aggregates and lipid bilayers
... fluorescence lifetime imaging mi- croscopy (FLIM) and atomic force microscopy (AFM) of trimeric LHCII adsorbed to mica substrates and ma- nipulated the environment to cause varying degrees of ...
11
Apertureless near field optical microscopy for fluorescence imaging
... effects. Fluorescence quen- ching could be identified by taking fluorescence lifetime measurements using a pulsed laser and measuring the delay between a laser pulse and the fluorescent photons ...
142
A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging
... of fluorescence lifetime data, whereby there is no longer a need to make a compromise between accuracy, speed and spatial ...cell imaging experiments to highlight the capabilities of the ...
19
Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre.
... trace). Fluorescence lifetimes of YFP in the whole cell samples were recorded to detect any quenching that would indicate Fo¨rster resonance energy transfer (FRET) from the YFP to an acceptor molecule ...
12
Nelsen_unc_0153D_18908.pdf
... phagocytosis where force drift was consistently low across the data set. Regardless, several of the curves that display low drift and have high quality imaging are consistent with the overall results from the data ...
141
Towards unsupervised fluorescence lifetime imaging using low dimensional variable projection
... the lifetime components are large, but can easily be biased when the lifetimes are comparable or even less than the FWHM of the ...the fluorescence lifetimes are short, biologists still prefer fitting ...
15