Fluorescence melting curve analysis
Original Article Hereditary spherocytosis combined with glucose-6-phosphate dehydrogenase deficiency in a Chinese family
... with fluorescence melting curve ...with fluorescence melting curve analysis revealed a 1388G>A mutation in the G6PD gene in the proband and his ...membrane ...
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Pathogenesis of Anti-HIV Drug (TENOFOVIR) Induced Renal Damage: Possible Roles of Oxidative Stress, Nitrosative Stress And Nf-Κb Pathway.
... The melting curve analysis showed the presence of a single peak figure 10 C GAPDH Figures 11: A-B show the log fluorescence data graph, standard curve and melting curves respectively fo[r] ...
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Simple Algorithm for Identification of Bordetella pertussis Pertactin Gene Variants
... of fluorescence at 495 nm at the end of each extension step allows the detection of the SYBR Green I bound to the amplified double-stranded ...amplification curve and the Ct level, calculated as the 10-fold ...
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Detection of Diarrheagenic Escherichia coli by Use of Melting Curve Analysis and Real Time Multiplex PCR
... The assay that we describe is unique in the number of dif- ferent genes recognized in a single reaction. Careful selection and testing of primers allowed this approach to be successful. The choice of some individual ...
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Detection and Differentiation of In Vitro Spiked Bacteria by Real Time PCR and Melting Curve Analysis
... Both fluorescence probes provided a correct Gram stain ...ml. Melting-curve analysis enabled further species-specific ...two melting points could be identified for all ...the ...
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High Resolution Melting Curve Analysis for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis Clinical Isolates
... High-resolution melting (HRM) curve analysis is a simple technique and has been widely used for many ...and fluorescence loss is monitored in ...in melting point (T m ) compared to ...
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Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real Time PCR and Melting Curve Analysis
... I melting curve analysis was used to confirm the specificity of the method when DNA extracted from fecal samples spiked with oocysts was ...I fluorescence and targeted a polymorphic coding ...
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Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real Time PCR and Two Step Melting Curve Analysis
... the fluorescence at the end of the annealing phase for each ...a melting curve was generated by holding the reaction mixture at 95°C for 10 s and then lowering the temper- ature to 48°C at a ...
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Ultrasensitive Quantification of Hepatitis B Virus A1762T/G1764A Mutant by a SimpleProbe PCR Using a Wild Type Selective PCR Blocker and a Primer Blocker Probe Partial Overlap Approach
... The fluorescence label is indicated by an underline (also see ...(with fluorescence acquisition), and 72°C for 5 ...a melting curve analysis over a linear temperature increase ...as ...
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Rapid Detection and Molecular Differentiation of Toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans Strains by LightCycler PCR
... The systemic symptoms of diphtheria are caused by the tox-encoded diphtheria toxin (DT) which is produced by toxigenic Corynebacterium spp. Besides the classical agent C. diphtheriae, the zoonotic pathogen C. ulcerans ...
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Detecting differential allelic expression using high-resolution melting curve analysis: application to the breast cancer susceptibility gene CHEK2
... high-resolution melting (HRM) analysis approach to perform allele-specific expression measurement in CHEK2 ...the fluorescence intensity of the probe, as it dissociates from the two allelic ...
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Direct Detection of Macrolide Resistance in Mycoplasma genitalium Isolates from Clinical Specimens from France by Use of Real Time PCR and Melting Curve Analysis
... Mycoplasma genitalium is a sexually transmitted organism commonly treated with azithromycin. However, macrolide resis- tance has been reported and is associated with point mutations in the 23S rRNA gene. To evaluate the ...
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Multiplex Real Time PCR Melting Curve Assay To Detect Drug Resistant Mutations of Mycobacterium tuberculosis
... The fluorescence detection relies on the difference in fluorescence emissions between the melted and hybridized configurations of the probe ...in fluorescence emission will be readily detectable when ...
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Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
... resolution melting (HRM) analysis has been employed, apart from other applications, also for fungal genotyping and differentiation (for review see Zambounis et ...revealing melting curve ...
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Sensitive Detection of Borrelia burgdorferi Sensu Lato DNA and Differentiation ofBorrelia Species by LightCycler PCR
... and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were ...by melting-curve analysis of the p66 gene ...by ...
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Cocirculation of Antigenic Variants and the Vaccine Type Virus during the 2004 2005 Influenza B Virus Epidemics in Japan
... In the 2004–2005 season, there was a large epidemic of the influenza B virus Yamagata group in Kobe, Japan. In hemagglutination inhibition tests, most of the clinical isolates from Kobe showed antigenicities similar to ...
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Numerical Investigation of Bismuth Unloading Solidification with Abnormal Melting Characteristic
... agreement with the Bridgman’s. Duff [5] and his collaborators first studied the shock induced phase transition of bismuth. They used two kinds of techniques of explosive load and electrical probe measurement speed, ...
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Detection and Differentiation of Human Polyomaviruses JC and BK by LightCycler PCR
... solution (25 mM; Roche Molecular Biochemicals; reagent 2), 2 pmol of sense primer, 8 pmol of antisense primer, 4 pmol of each probe, and 5 l of target DNA. Each mixture was made up to 20 l using sterile PCR-grade water ...
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Spectrofluorimetric Determination of Flutamide in Pharmaceutical Preaparations
... calibration curve was obtained by plotting the intensity of fluorescence of the flutamide versus analyte ...regression analysis of the calibration curve showed a linear relationship between ...
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Biological Evaluation and Molecular Docking of Substituted Quinazolinones as Antimicrobial Agents
... the melting curve analysis revealed that compound II affected gene codes for large subunit rRNA in both Geotrichum candidum and Candida albicans, since it decreased the Tm of DNA of treated fungi as ...
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