Functional complementation with AcCOL2 using Arabidopsis
Functional complementation of pop2-3 T-DNA insertion mutant of Arabidopsis thaliana by overexpression of gamma-aminobutyric acid transaminase gene to reveals its role in complex pattern of leaf senescence
... performed functional complementation using transformation of pPZP200GB-GABA-T construct into pop2-3 T-DNA insertion mutant (GABA-T knockout mutant) of ...
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Functional Conservation of the Yeast and Arabidopsis RAD54-Like Genes
... of functional complementation oc- curs between plant and yeast genes in both organisms: the plant gene AtRAD54 in yeast cells and the yeast gene RAD54 in ...and Arabidopsis is demon- F igure ...
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Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris
... Results: In order to efficiently screen the IRES sequences, we first set out to create a screening system using LacZ gene. Due to the cryptic transcription of the LacZ gene, we established the ...
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Functional analysis of archaeal MBF1 by complementation studies in yeast
... the complementation experiments using archaeal- yeast chimeric proteins presented here suggests that archaeal MBF1 is not able to functionally interact with the transcription machinery and/or Gcn4 of ...
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Functional Characterization of Pneumocystis carinii brl1 by Transspecies Complementation Analysis
... FIG. 3. P. carinii Brl1p rescues the cell cycle arrest phenotype of the S. pombe brl1 null mutant. (A) The S. pombe diploid brl1 null mutant was sporulated, the four spores of each tetrad were separated on rich medium, ...
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Functional Evaluation of the Mechanisms of Reactive Oxygen Detoxification in the Hyperthermophilic Archaeon Pyrococcus Furiosus Using Complementation Studies
... 19 As an alternate method, the cloning vector, pPCR-Script was employed to clone these two genes as a single sequence. Because the restriction enzyme sites of the Rd-SOR PCR product are located at the ends of the DNA ...
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Functional characterization of Arabidopsis sorting nexin AtSNX2b
... curved structures that can sense membrane curvature (Habermann, 2004; Peter et al., 2004; Ren et al., 2006) and, together with the PX domain, they target the SNX to specific phosphatidylinositol-lipid-rich organelles. ...
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Molecular and Functional Analysis of Volatile Isoprenoids in Arabidopsis
... protein using primers P50 and P51 for initial PCR ...microscopy using a LSM 510 microscope (Carl Zeiss, Jena, Germany) equipped with a HeNe ...image using the Zeiss LSM Image Browser ...
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Functional analysis of a reticulon protein from Arabidopsis thaliana
... the functional relevance of this characteristic, and indeed its mechanism, remain ...kDa. Using pulse-chase analysis, the protein was observed to be stable over at least six hours as there was no evidence ...
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Functional characterization of Arabidopsis ARGONAUTE 3 in reproductive tissue
... RNA-directed-DNA-methylation. Using full- locus constructs, we characterized the expression, biochemical properties, and possible modes of action of ...most Arabidopsis AGOs - AGO2 included - it binds ...
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Functional characterization of the HUA2 gene family in Arabidopsis thaliana
... development. Using different protein- protein interaction techniques such as the yeast two-hybrid assay and GST pull-down assay, my first objective is to identify proteins interacting with HUA2 followed by mapping ...
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Functional Analysis of the miR156 Regulatory Network in Arabidopsis Siliques
... The Arabidopsis genomic DNA was extract by cetyltrimethyl ammonium bromide (CTAB) method (Allen et al., 2006). Plant leaf tissue was ground in liquid nitrogen using a cold mortar and pestle. The powder was ...
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Complementation of wild strawberry (Fragaria vesca L.) SPATULA (FvSPT) and SPIRAL (FvSPR) genes in Arabidopsis thaliana
... The complementation test is a very effi- cient tool for functional genomic analysis. In the plant kingdom, the model plant A. thaliana, with its well-known genome, has plenty of natural and induced mutants, ...
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Partial Functional Complementation between Human and Mouse Cytomegalovirus Chemokine Receptor Homologues
... PFU/organ) and standard deviations are shown. Samples with no detectable virus were assigned a titer equivalent to the threshold of detection for the plaque assay. The detection threshold for each organ is indicated as a ...
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Functional network construction in Arabidopsis using rule based machine learning on large scale data sets
... Generated Using the BioHEL Algorithm Despite a given rule set generated using BioHEL being very accurate, it does not represent the only possible model predictor of developmental outcome that can be ...
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Improving total saccharification yield of Arabidopsis plants by vessel-specific complementation of caffeoyl shikimate esterase (cse) mutants
... Similar to many lignin-deficient mutants in Arabidop- sis, the cell walls of cse-2 exhibit a higher saccharification efficiency compared to the wild type, but total biomass is significantly reduced. By restoring the ...
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Prediction, validation and functional analysis of mirna targets in Arabidopsis thaliana
... predict Arabidopsis miRNA targets, and to sort them according to the calculated MFE of the hybrid ...same Arabidopsis dataset and the same ...example, using an MEF cutoff of 70%, there are ...
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An Integrated Map of Arabidopsis thaliana for Functional Analysis of Its Genome Sequence
... species Arabidopsis thaliana has recently been ...of Arabidopsis. Accuracy of the integrated map was verified using the existing physical and sequence maps and numerous DNA ...facilitate ...
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Cluster analysis of soybean pathogen-responsive genes and functional characterization in Arabidopsis
... 50 II. INTRODUCTION A major goal in plant breeding is to identify sequences that are associated with a trait. One can identify these genetic regions by producing random DNA markers that are polymorphic between parents, ...
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Interallelic Complementation at the Drosophila melanogaster gastrulation defective Locus Defines Discrete Functional Domains of the Protein
... 11A, was first described in a genetic screen for maternal to assay for activities of discrete domains of Gd. Our data support processing of Gd in a manner consistent effect genes affecting embryonic development (Moh- ler ...
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