Gal4 DNA binding domain
![An X11[alpha]/FSBP complex represses transcription of the GSK3[beta] gene promoter](data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==)
An X11[alpha]/FSBP complex represses transcription of the GSK3[beta] gene promoter
... PDZ2 domain “wrecking” mutants were generated by mutating the key ILGV (residues 666-669 in PDZ1) and QLGF (residues 778-781 in PDZ2) to ...intracellular domain (AICD)-dependent transcription and GSK3 ...
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Neotendon formation induced by manipulation of the Smad8 signalling pathway in mesenchymal stem cells
... MH2 domain, Smad8 har- bors an SSVS motif that has the potential to be phosphorylated for Smad activation (23, ...the GAL4 DNA-binding ...heterologous DNA (UAS) by means of the ...
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Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 4 (vIRF4/K10) Is a Novel Interaction Partner of CSL/CBF1, the Major Downstream Effector of Notch Signaling
... brary of KSHV fused to the GAL4 activation domain (40, 8). The CBF1 sequence was fused to the GAL4 DNA binding domain and used as bait. Out of a group of several candidates, ...
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EBP2, a Human Protein That Interacts with Sequences of the Epstein-Barr Virus Nuclear Antigen 1 Important for Plasmid Maintenance
... the GAL4 DNA binding domain (amino acids 1 to ...the GAL4 DNA binding domain and a hem- agglutinin (HA) epitope were constructed by inserting the EBNA1 fragment ...
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Characterization of the Epstein-Barr virus BZLF1 protein transactivation domain.
... However, other features of Zta may be important for activity since a Gal4-Zta chimeric protein, generated by fusing the amino-terminal 167 residues of Zta to the DNA binding domain of th[r] ...
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The Interaction of Bacteriophage P2 B Protein with Escherichia coli DnaB Helicase
... the addition of X-Gal. The colors of the colonies were re- corded after incubation at 30°C for 1 h. These were dark blue for cells with the positive control plasmid, blue for cells ex- pressing both fusion proteins, and ...
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Dimerization of the Papillomavirus E2 Protein Is Required for Efficient Mitotic Chromosome Association and Brd4 Binding
... N-terminal domain is both necessary and sufficient for association with mitotic chromosomes ...terminal domain is found to be associated with mitotic chro- mosomes much less frequently than the full-length ...
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The transcriptional activation domain of varicella-zoster virus open reading frame 62 protein is not conserved with its herpes simplex virus homolog.
... Plasmid GALA contains the DNA binding domain amino acids 1 to 147 [18] of the yeast GAL4 protein preceded by the simian virus 40 early promoter and followed by a polylinker sequence 29..[r] ...
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Comparing regions of the Epstein-Barr virus ZEBRA protein which function as transcriptional activating sequences in Saccharomyces cerevisiae and in B cells.
... cerevisiae, the intact protein and subdomains of ZEBRA fused to the DNA binding domain of GAL4 were tested for their capacity to activate transcription in a yeast strain containing a mut[r] ...
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Modularity of eukaryotic transcription activators
... the DNA-binding domain. Bringing the activating region to DNA by a protein-protein interaction triggers activation of ...inhibits Gal4 by binding to and covering Gal4's ...
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Specificity and sequence requirements for interactions between various retroviral Gag proteins.
... ,B-gal activity was assessed in yeast cells individually transformed with plasmids expressing GAL4-mutant Gag fusion proteins including both GAL4 DNA-binding and activation domain fusion[r] ...
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An Epstein-Barr virus nuclear protein 2 domain essential for transformation is a direct transcriptional activator.
... To determine whether EBNA-2 has a direct transcriptional activating domain, gene fusions between the DNA-binding domain of GAL4 and EBNA-2 were tested in CHO and B-lymphoma cells for the[r] ...
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Multiple regions within EBNA1 can link DNAs.
... linking domain within EBNA1 acts independently of other regions of ...of GAL4-EBNA1 fusion proteins ...and DNA-binding domain of ...a DNA with five GAL4-binding ...
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Monoclonal Antibodies against the Minimal DNA-Binding Domain in the Carboxyl-Terminal Region of Human Immunodeficiency Virus Type 1 Integrase
... Production of MAbs against IN. Female BALB/c mice were immunized primarily with HIVIN fused to Escherichia coli maltose-binding protein (MBP) and thereafter with HIVIN, with the N-terminal 20 aa residues ...
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Identification of a Functionally Relevant Adeno-Associated Virus Rep68 Oligomeric Interface
... viral DNA replication cycle with the assistance of the host cell machinery (12, ...the DNA binding and endonuclease activities are required for mediation of integration at chromo- somal target loci ...
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Ludwigsen, Johanna (2017): ATP-dependent nucleosome remodeling by ISWI: insights into mechanism and regulation. Dissertation, LMU München: Fakultät für Biologie
... proper binding site on the nucleosome. With regards to how DNA enters the nucleosome, we propose that DNA ratchets into the nucleosome once the tension that builds up by extruding base pairs (bp) ...
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Activation domain requirements for disruption of Epstein-Barr virus latency by ZEBRA.
... the first lane) and subjected to Southern blot analysis with a chemiluminescence detection kit (ECL; Amersham). Plasmid p526, which contains the EBV lytic origin of replication, was used as a probe. For demonstration ...
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The DNA Binding Domain of a Papillomavirus E2 Protein Programs a Chimeric Nuclease To Cleave Integrated Human Papillomavirus DNA in HeLa Cervical Carcinoma Cells
... BEF-induced DNA double-strand break near E2 BS4, another high-affinity BS in integrated HPV18 DNA in HeLa ...a DNA double-strand break in the HPV18 DNA near E2 BS4 ...by DNA from cells ...
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Characterisation of a bagpipe homologue in Xenopus laevis
... reporter construct containing GAL4 DNA binding sites upstream of an SV40 promoter controlling firefly luciferase, in transfected ES cells. x axis Ga14 fusion transfected. [r] ...
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Mapping the Sequences That Mediate Interaction of the Equine Herpesvirus 1 Immediate-Early Protein and Human TFIIB
... in binding TFIIB than the full-length IE ...the DNA-binding domain (aa 422 to 597) may harbor the major site for interaction with TFIIB, since IE⌬621–757 appeared to interact more efficiently ...
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