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Gene identification and isolation

IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING APARTIALANNEXIN GENE FROM AUREOBASIDIUM PULLULANS

IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING APARTIALANNEXIN GENE FROM AUREOBASIDIUM PULLULANS

... During the last two decades with advances in medi­ cine, mycoses due to opportunistic fungi are increasing dramatically andA. pullulans is considered one of them. The increased frequency of organ transplantations, the ...

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Rapid isolation of gene homologs across taxa: Efficient identification and isolation of gene orthologs from non-model organism genomes, a technical report

Rapid isolation of gene homologs across taxa: Efficient identification and isolation of gene orthologs from non-model organism genomes, a technical report

... or gene networks to study molecular evolution across divergent taxa or within specific ...large gene families, allowing for a comparative analysis over millions of years of evolutionary ...

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Isolation, sequence identification and tissue expression  profile of a novel goat gene-RAP1B

Isolation, sequence identification and tissue expression profile of a novel goat gene-RAP1B

... goat gene, RAP1B, was amplified using the rapid amplification of cDNA ends (RACE) method based on one goat EST sequence which was highly homologous to the coding sequence of human RAP1B ...this gene encodes ...

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Isolation and Identification of miRNAs in Jatropha curcas

Isolation and Identification of miRNAs in Jatropha curcas

... Abstract MicroRNAs (miRNAs) are small noncoding RNAs that play crucial regulatory roles by tar- geting mRNAs for silencing. To identify miRNAs in Jatropha curcas L, a bioenergy crop, cDNA clones from two small RNA ...

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Isolation and Identification of a Sulfide/Sulfoxide Monooxygenase Gene from a Newly Isolated Rhodococcus Sp. Strain FMF

Isolation and Identification of a Sulfide/Sulfoxide Monooxygenase Gene from a Newly Isolated Rhodococcus Sp. Strain FMF

... dszC gene from Rhodococcus FMF was identified and then isolated by PCR technique using the nucleotide sequence of dszC gene from Rhodococcus erythropolis IGTS8 to design required ...this gene ...

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Isolation, Identification, Detection of Bacteria from Meat, Fish, Poultry Samples and Detection of Staphylococcus Aureus Enterotoxin Gene

Isolation, Identification, Detection of Bacteria from Meat, Fish, Poultry Samples and Detection of Staphylococcus Aureus Enterotoxin Gene

... Chemicals Crystal violet, Grams iodine, ethanol, safranin, were used for morphological identification. The media and reagents used for the study such as Nutrient Broth, Nutrient agar, Eosin-Methylene Blue agar, ...

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Isolation, Identification and Growth Optimisation of Freshwater Microalgae

Isolation, Identification and Growth Optimisation of Freshwater Microalgae

... the isolation and evaluation of the microalgae based on their morphology, 18S rDNA gene and ITS (Internal Yaoyao Xu 1 , Aoxue Lu 1 , Xinyue Chen 1 , Dongyang Su 1 , Weimin Wang 1 , Yongjun Zhang ...

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Identification and Isolation of an Azoreductase from Enterococcus faecium

Identification and Isolation of an Azoreductase from Enterococcus faecium

... acpD gene product (AzoEf1) responsible for the azoreductase activity of Enterococcus faecium, an intestinal bacterium, was heterologously expressed, purified and ...

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Different Methods for Isolation and Preliminary Identification of Azotobacter

Different Methods for Isolation and Preliminary Identification of Azotobacter

... For molecular analysis, one isolete was grown for 2–3 days on LG. Crude template DNA was extracted using alkaline lysis method(Rademaker & de Bruijin, 1997). The 16S rRNA gene was amplified by means of ...

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Isolation and Identification of Nah-B Gene from Bacteria Isolated from  Effluents & Testing its Hydrocarbon Degrading Efficiency

Isolation and Identification of Nah-B Gene from Bacteria Isolated from Effluents & Testing its Hydrocarbon Degrading Efficiency

... One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products are of particular concern in the ...

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Isolation of an Apoptosis Suppressor Gene of theSpodoptera littoralis Nucleopolyhedrovirus

Isolation of an Apoptosis Suppressor Gene of theSpodoptera littoralis Nucleopolyhedrovirus

... suppressor gene in the SlNPV genome. We report here the identification of Slp49, a func- tional antiapoptotic gene of SlNPV, isolated from an SlNPV cosmid library by complementing the replication of ...

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ISOLATION AND IDENTIFICATION OF BACILLUS Sp.

ISOLATION AND IDENTIFICATION OF BACILLUS Sp.

... The isolates RR2, RR8, RR9 and RR10 are identified as Bacillus sp. by staining and biochemical techniques. Genomic identification of the Bacillus sp. was identified by 16S rRNA. All the fifteen different bacteria ...

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Isolation and identification of ligninolytic microbes

Isolation and identification of ligninolytic microbes

... isolateligninolytic microbes from various niche and to screen them for potential ligninolytic activity. The lignin extract was prepared from saw dust. The ligninolytic bacteria were isolated from of garden soil, sewage ...

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The isolation and characterisation of the novel gene C53

The isolation and characterisation of the novel gene C53

... generate gene expression profiles for molecules that are known to be important in directing the progression through the haematopoietic developmental ...in gene expression during complex developmental ...

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Isolation, identification, and characterization of novel nanovesicles

Isolation, identification, and characterization of novel nanovesicles

... for isolation and purification, followed by analytic approaches that generate data that may not represent what takes place in ...the identification of previously unrecognized HG-NVs may also increase our ...

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Isolation and identification of concrete environment bacteria

Isolation and identification of concrete environment bacteria

... Bacteria isolation and enrichment 2.1.1 Isolation The bacteria used in this study are isolated from fresh urine and acid mine water, Kota Tinggi, Johor, ...The isolation process of both ureolytic and ...

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Isolation And Identification Of Steroides From E.Hirta

Isolation And Identification Of Steroides From E.Hirta

... Isolation And Identification Of Steroides From E.Hirta Prity Sharma, Shushila Dhaka Abstract : Euphorbia hirta Schrad. is an important medicinal plant belonging to family Euphorbiacae. Some phytochemicals ...

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Isolation and Identification of Cellulose Degrading Microbes

Isolation and Identification of Cellulose Degrading Microbes

... V. CONCLUSION The current study was based on the comparative study of cellulase degrading bacteria and fungi. The bacterial identification was done by Bergey’s manual of determination and fungi ...

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Isolation and Identification of Naphthalene Degradation Bacteria

Isolation and Identification of Naphthalene Degradation Bacteria

... IV. IDENTIFICATION OF SELECTED BACTERIAL ISOLATES The morphological and biochemical characterization of the bacterial isolates were identified NS1A as Staphylococcus aurous and MM3 as Pseudomonas ...

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Isolation and identification of microorganisms in acne patients

Isolation and identification of microorganisms in acne patients

... Sabouraud’s dextrose agar, were used for fungi. This medium supports the growth of most fungi. Pure colonies were identified using morphological, biochemical, physiological characteristics. The newly redesigned ...

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