Gene knockdown
RNAi mediated gene knockdown by microinjection in the model entomopathogenic nematode Heterorhabditis bacteriophora
... investigate gene function, which are unavailable for most parasitic ...of gene function [14]. RNAi is the RNA-induced silencing of gene-specific mRNA targets ...to knockdown al- most all the ...
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RNAi-based conditional gene knockdown in mice using a U6 promoter driven vector
... target gene allows formation of a complex comprising one strand of the shRNA or siRNA hybridized with the mRNA target and the RISC (RNAi-induced silencing complex) proteins in the ...corresponding gene ...
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Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown
... In the present study we report a novel synthetic route for a new class of cationic polymethacrylates. This involved the synthesis of water soluble, phosphonium containing methacrylate monomers – and their corresponding ...
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A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
... ABSTRACT CRISPR interference (CRISPRi) is a powerful new tool used in different organisms that provides a fast, specific, and reliable way to knock down gene ex- pression. Caulobacter crescentus is a well-studied ...
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Estrogen Receptor Alpha 36 Gene Knockdown Promote the Expression of NF κB in PC12 Cells
... ER-α36 gene silencing on the expression of NF-κB in normal cultured PC12 cells and to provide an experimental foundation for understanding the function of ER-α36 in nerve ...ER-α36 gene silencing can ...
5
Si-RNA mediated knockdown of CELF1 gene suppressed the proliferation of human lung cancer cells
... Lung cancer cells tend to form large cell colonies while in culture; therefore, we next evaluated the effect of CELF1 silencing on the colony forming ability of lung cancer cells. As shown in Figure 5A and B, the size of ...
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Allele specific RNA interference rescues the long QT syndrome phenotype in human induced pluripotency stem cell cardiomyocytes
... mRNA knockdown by A13-siRNA was confirmed in fibroblast transgenic lines (Figure 4Ai), and repeated transfection with siRNA every 48 h for 6 days sustained knockdown of Mut KCNH2 (Figure ...61.8% ...
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Nardilysin controls intestinal tumorigenesis through HDAC1/p53–dependent transcriptional regulation
... Specific gene knockdown of HDAC1 also abrogated the enhance- ment of p53 acetylation and cell apoptosis by NRDC gene knockdown (Figure ...
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Original Article Small interfering RNA-mediated inhibition of respiratory syncytial virus infections in Hela cells
... Hela cells were cultured using the above- mentioned methods. Cultured cells were divided into 5 groups and transfected with pre- prepared compounds, including the blank con- trol group (32.5 μl DMEM), negative control ...
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SALL4 activates TGF-β/SMAD signaling pathway to induce EMT and promote gastric cancer metastasis
... 1 gene knockdown was shown in Figure 5A and ...1 gene knockdown group displayed reduced abilities of cell motility, migration, and invasion (Figure 5C and ...1 gene knockdown ...
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5428.pdf
... shRNA gene knockdown are an effective validation strategy; 2) of the candidate genes tested, PELI2 (described further below) is the strongest candidate for further studies investigating its role in ...
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Concerted Transcriptional Regulation by BRCA1 and COBRA1 in Breast Cancer Cells
... susceptibility gene product ...(shRNA)-mediated gene knockdown and gene expression microarray were used to study the impact of COBRA1 and BRCA1 on global transcription in the same breast ...
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RNA Interference-Mediated Knockdown on DKC1 Gene in Chemosensitized Colorectal Cancer Cell Lines
... DKC1 gene was checked at mRNA level by qPCR using a Rotor-Gene RG-6000 Real-Time Thermal Cycler (Corbett Research, Sydney, Australia) utilizing the Solaris Human qPCR Gene Expression Assay (Thermo ...
5
Initiation of trophectoderm lineage specification in mouse embryos is independent of Cdx2
... apicobasal polarity. Examination of Cdx2-deficient blastocysts under the electron microscope (Fig. 1G) showed that the tight junction structure was actually missing, although ZO-1 protein was Fig. 1. Efficient reduction ...
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CBP mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing
... To further explore this antagonism, we also determined whether simultaneous E(Z) overexpression could compensate for the effects of CBP overexpression. Although a high level of ubiquitous CBP overexpression is lethal, ...
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Original Article RNAi-mediated knockdown of RBPJ gene inhibits the growth of human breast cancer cells
... that knockdown of RBPJ expres- sion by RNA interference (RNAi) inhibited the anchorage-independent growth of rhabdomyo- sarcoma cells and the growth of xenografts in vivo [24], which suggested that interruption of ...
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Knockdown Arguments
... baculum fallacy independently of the other elements. And the third factor is built on top of the second: threats aren't good reasons in the type of dialogue that the sales[r] ...
21
Cdx ParaHox genes acquired distinct developmental roles after gene duplication in vertebrate evolution
... extensive gene loss following genome duplication, such that only a proportion of genes are retained in multiple ...of gene loss, the result is consistent with pre-genomic analyses, since the first strong ...
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siRNA Knockdown of Ribosomal Protein Gene RPL19 Abrogates the Aggressive Phenotype of Human Prostate Cancer
... the data indicate the effects to be selective with respect to cell adhesion, stromal invasion and tumorigenesis. Although the role of individual RPs in determining the cellular phenotype of eukaryotic cells remains ...
16
Differentiation of breast cancer stem cells by knockdown of CD44: promising differentiation therapy
... CD44 knockdown BCSCs lost the BCSC phenotype and showed reduced expression of genes related to stemness, metastasis and tumorigenesis, espe- cially Muc-1 and Bcl-2 ...RNAi gene therapy could provide a novel ...
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