Gene Product Identification using the Maxi-Cell
env Gene of Rous sarcoma virus: identification of the gene product by cell-free translation.
... Poly(A)-selected, dena- tured virion 70S RNAs were translated in a messen- ger-dependent rabbit reticulocyte lysate, and the products were analyzed by electrophoresis on a 10%. polyacryl[r] ...
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Identification and localization of polycystin, the PKD1 gene product
... Localization of polycystin in normal tissues and ADPKD kidneys. MR3-Ab gave intense staining in tissue sections whereas the other two antibodies gave weaker but similar re- sults; MR3-Ab was, therefore, used in ...
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Identification and characterization of the bovine herpesvirus 1 UL7 gene and gene product which are not essential for virus replication in cell culture.
... UL7 gene is not substantially transcribed into stable mRNA during the late phase of the infection and supports the conclusions drawn from the Northern blot ...
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Identification of clear cell renal cell carcinoma and oncocytoma using a three gene promoter methylation panel
... Conclusions A panel including OXR, MST1R and HOXA9 promoter methylation might be useful for positive identification of RCT, as well as for discrimination among subtypes. This panel could be used as ancillary ...
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Identification and characterization of duck plague virus glycoprotein C gene and gene product
... gC gene FQ-PCRs were used to detect the expression of the DPV gC gene during viral ...synthesized using reverse ...gC gene or for ...the gene expression determined from quantitative ...
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Identification of a gene signature in cell cycle pathway for breast cancer prognosis using gene expression profiling data
... of gene expression signatures based on microarray data to predict prognosis and responses to chemotherapy in cancers ...multiplex gene expression markers are more powerful in predicting clinical outcomes ...
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Identification of novel stem cell markers using gap analysis of gene expression data
... whether development of an mRNA or protein molecular marker is feasible. Our gap method stands apart from existing methods of microarray clustering. Standard clustering algorithms such as hierarchical or k-means ...
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Unbiased Boolean analysis of public gene expression data for cell cycle gene identification.
... public gene expression data for cell cycle gene identification ABSTRACT Cell proliferation is essential for the development and maintenance of all organ- isms and is dysregulated in ...
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Identification of the Pr1 Gene Product Completes the Anthocyanin Biosynthesis Pathway of Maize
... encoding gene (Zmf39h1) and showed by segregation analysis that the red kernel phenotype is linked to this ...mapping using SNP markers confirms its position on chromosome ...experiments using a CaMV ...
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Identification of meat spoilage gene biomarkers in Pseudomonas putida using gene profiling
... 1. Introduction Meat represents one of the most important consumed food commodities worldwide. Food availability data indicate that overall meat consumption in developed countries is increasing, with the USA standing as ...
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Identification and functional characterisation of gene fusions in human cancer cell lines
... cancer cell line panel was established in the 1980’s by the National Cancer Institute in the USA, in the form of the ...cancer cell lines of 9 distinct tissue types that were characterised by karyotype ...
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Identification and Characterization of the UL56 Gene Product of Herpes Simplex Virus Type 2
... Plasmid transfection. Cells were transfected by using the Lipofectamine re- agent according to the protocols recommended by the supplier (Gibco-BRL). Preparation of polyclonal antisera. Antisera were produced in ...
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B-cell malignancies: capture-sequencing strategies for identification of gene rearrangements and translocations into immunoglobulin gene loci
... This approach has been tested in ALL where it was able to detect MRD. 10 As numerous correlative studies suggest, in ALL the prognostic power of MRD could also increase due to the higher sensitivity of this approach. ...
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The Herpes Simplex Virus 1 UL51 Gene Product Has Cell Type-Specific Functions in Cell-to-Cell Spread
... sequence using the Eukaryotic Linear Motif online resource (24) revealed several membrane-trafficking motifs that might be expected to play a role in virion or virus glycoprotein sorting for ...
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FISH IDENTIFICATION USING SEQUENCE ANALYSIS OF THE AMPLIFIED COI GENE
... The PCR product was purified using AxyPrep PCR Clean up kit (Axygen, AP-PCR-50). 100 µl of PCR-A buffer was added to the 25 µl of reaction. The sample was mixed and transferred to column placed in 2 ml ...
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Identification of Hodgkin and Reed Sternberg cell specific genes by gene expression profiling
... phenotype. Using microarrays, we compared the gene expres- sion profile of four HL cell lines with profiles of the main B cell subsets and B cell non-HLs to find out whether HRS cells, ...
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Identification and validation of a 44-gene expression signature for the classification of renal cell carcinomas
... markers. Using microarray analysis of renal tumours, claudin-7 mRNA, a distal nephron marker, was overex- pressed in chRCC compared with that in oncocytoma, ccRCC, and pRCC ...diseases using a single ...
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Identification of the EnteropathogensCampylobacter jejuni and Campylobacter coli Based on the cadF Virulence Gene and Its Product
... jejuni clinical isolates belong to biotype I or II. Lior noted that only 4.0 and 2.7% of the 1,195 C. jejuni isolates from humans were found to be biotype III and biotype IV, respectively. Most of the C. jejuni and C. ...
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Maxi Pressurisation Units (Maxi, Maxi Plus & HP)
... Mikrofill Maxi requires a permanent 230V 50Hz 1 phase supply rated at 6 ...The Maxi also incorporates two BMS relays offering volt free contacts for the remote indication of high or low pressure conditions ...
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Identification of a bovine rotavirus gene and gene product influencing cellular attachment.
... Preliminary characterization of naturally occurring temperature-sensitive bovine rotavirus mutants, gener- ated via gene reassortment, indicated that the product of gene segment 10 can i[r] ...
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