gene trap
Female gametophytic mutants of Arabidopsis thaliana identified in a gene trap insertional mutagenesis screen
... reporter gene expression under the promot- ers of the disrupted genes: The gene trap GUS gene was engineered into the Ds, preceded by multiple splice acceptor sites and it has no promoter, so ...
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Dual-Tagging Gene Trap of Novel Genes in Drosophila melanogaster
... A gene-trap system is established for ...the gene-trap system allows the recovery only of fly lines whose genes are inactivated by a P-element insertion, ...the gene-trap system, ...
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An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells
... RCASBP-M2C-based gene trap vector, pGT- GFP, which allows the prescreening of mutagenized cells based on the subcellular localization of the GFP ...In gene trap experiments in both yeast (8) ...
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Expression from a betageo gene trap in the Slain1 gene locus is predominantly associated with the developing nervous system
... lacZ gene itself, such as transcriptional instability due to its high G + C content (Montoliu et ...in gene trap or knock in experi- ...independent gene trap lines, it is still likely ...
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Essential validation of gene trap mouse ES cell lines: a test case with the gene Ttrap
... Because the β Geo-encoding sequence is under the transcrip- tional control of the endogenous Ttrap promoter, we wanted to document the Ttrap gene expression domain during embryogen- esis by staining for β ...
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Early embryonic lethality in gene trap mice with disruption of the Arfgef2 gene
... Heterozygous males and females were mated. The day of vaginal plug was counted as day E0.5. Fertilized oocytes (syngamy stage) were collected at day E0.5 and tested for pronuclear stages using light microscopy. ...
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A piggyBac Transposon Gene Trap for the Analysis of Gene Expression and Function in Drosophila
... P-element-based gene and enhancer trap strategies have provided a wealth of information on the expression and function of genes in Drosophila ...site gene trap vector (PBss) was accomplished ...
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A large-scale gene-trap screen for insertional mutations in developmentally regulated genes in mice.
... After examining the chimeras fi-om the 36 lines that showed spatially restricted patterns of expression in the 8.5 dpc embryos, we classified the expression patterns int[r] ...
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Enlarged dendritic spines and pronounced neophobia in mice lacking the PSD protein RICH2
... Rich2 gene (Arhgap44 Rho GTPase activating protein 44 [Mus musculus]; NCBI Gene ID: ...the gene trap vector in exon 1 is ...A gene-dosage effect by knock-out of a single copy of RICH2 ...
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Long noncoding RNAs, emerging players in muscle differentiation and disease
... Gtl2/Meg3: gene trap locus 2/maternally expressed gene 3; H3K27ac: acetylated histone H3 lysine 27; H3K4me1: monomethyl histone H3 lysine 4; IMP: Igf2 mRNA binding-protein; iPSC: induced pluripotent ...
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An investigation of the link between the bacterial derived queuine molecule and tyrosine production
... The decrease in tyrosine synthesis in queuine deficient HepG2 cells and TGT gene-trap mice was not due to changes in the activity or expression of the phenylalanine hydroxylase enzyme a [r] ...
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LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting
... ble gene regulation, but each has ...reversible gene reg- ulation, but it is difficult to recapitulate the expression levels of the endogenous genes with the synthetic activa- tors ...reversible gene ...
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The trappist's approach to pathfinding: elucidating brain wiring using secretory trap mutagenesis
... secretory gene traps. (a) In this example, the classical gene-trap vector inserts into a gene (in this case encoding a protein without a leader sequence) between exons 2 and ...target ...
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A versatile strategy for gene trapping and trap conversion in emerging model organisms
... a gene trap has been isolated, integrase-mediated trap conversion (iTRAC) utilises attP as a docking site for integrating new constructs into the trapped ...DsRed trap is converted into one ...
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The proliferative human monocyte subpopulation contains osteoclast precursors
... medium (α-MEM and 3% Hi-FBS) in the presence of M-CSF (8,000 U/mL) with or without RANKL (50 ng/mL) in 6-cm tis- sue culture dishes (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). Cells were incubated for 14 ...
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Homemade Traps and Snares pdf
... the trap which can yeild better results there are metal stains available or although somewhat expensive powdercoating the ...the trap away from oil and petrol in the back of your ute and where gloves when ...
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The Development Of An Active Grease Trap For Domestic Application
... grease trap has caused this damage. Grease trap is a structure with special mechanism to trap the FOG in the ...grease trap. There are two types of grease trap, one is active grease ...
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System Trap rainwater engineering and lock brick technology in household scope to improve quality of life
... The system-track-rainwater engineering could be provided the availability of water to meet household water and agriculture-yard requirement. It means that the household economy will increase as whereas the quality of ...
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Evaluation of the effectiveness of introducing new alleles into the gene pool of a rare dog breed: Polish Hound as the example
... a gene pool of a rare dog breed by breeding use of dogs of unknown origin that are phenotypically similar to a given ...the gene pool and to the number of their descendants used in ...enriching gene ...
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Aberrant ADAM10 expression correlates with osteosarcoma progression
... (Ig) gene superfamily and plays an important role in a number of endothelial cell functions, including angiogenesis, in- flammation, integrin activation and intercellular adhesion [13-15], which could also be ...
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