Genes selected for qPCR analysis
Reference Genes for RT qPCR Analysis of Environmentally and Developmentally Regulated Gene Expression in Alfalfa
... the analysis of differences in gene expression in response to experimental treat- ments or among genetic ...erence genes stably expressed under various ...were selected based on their previous ...
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TCDD-induced changes in the expression of selected hypothalamic feeding-regulatory genes and mRNA quantification using reverse transcription qPCR
... Untangling the mechanisms of wasting syndrome provides information on an important facet of acute dioxin toxicity and sheds light on some of its processes, from transcription to pathology. An additional benefit is the ...
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Selection of reference genes for qPCR in hairy root cultures of peanut
... reference genes were evaluated. Nineteen genes were selected based on previous qPCR studies in plants and two were from the T-DNAs transferred from ...candidate genes were obtained ...
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Validation of Common Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process
... Candidate genes and primer ...keeping genes chosen for this study, as well as 3 target genes (Nanog, Rex1, and Oct4) used for further validation, and the selected primer pairs used for ...by ...
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Improving RNAi in the Brown Marmorated Stink Bug: Identification of Target Genes and Reference Genes for RT-qPCR
... reference genes. Also, use of multiple reference genes is impor- tant because using a single reference gene may not be sufficient to control variability across all treatments 34 ...reference genes ...
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Research Article Selection and Validation of Reference Genes for RT-qPCR Analysis in Spinacia oleracea under Abiotic Stress
... housekeeping genes in spinach (Spinacia oleracea) under various abiotic stresses is ...candidate genes and determining the optimal gene(s) for normalization of gene expression in spinach are necessary to ...
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RNAi of selected insect genes
... RT-qPCR analysis showed higher relative expression levels of this transcript in the most virulent RWA biotype, SAM, when compared to the least virulent biotype, SA1 (Figure ...
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Reference genes identification for normalization of qPCR under multiple stresses in Hordeum brevisubulatum
... general, genes that play key roles in the maintenance of basic cellular functioning are typically selected as ref- erence genes such as 18S ribosomal RNA (18SrRNA), beta-actin (β-actin), elongation ...
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Evaluation of reference genes for RT-QPCR studies in the seagrass Zostera muelleri exposed to light limitation
... are selected and appropriate primers ...reference genes needed for normali- zation of the target gene and (ii) the average expression stability of tested reference genes 48 ...reference genes ...
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The dilution effect and the importance of selecting the right internal control genes for RT qPCR: a paradigmatic approach in fetal sheep
... any genes, including housekeeping genes, thought to be stable across different tissue types, ages and treatment protocols, can be regulated at transcriptomic ...ICG selected based on absence of any ...
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Validation of reference genes for quantitative real time PCR (qPCR) analysis of Actinobacillus suis
... ence genes for ...reference genes used to study closely related organisms were not ...reference genes was assessed in different growth conditions, growth phases, and with various methods of data ...
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Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements.
... The genes with the lowest M values in this study were UBQ10 and Act (M = ...reference genes and, thereby, to evaluate the expression stability and provide a direct measure of variation (Andersen et ...
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Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress
... of genes that were sta- bly expressed under drought stress, including many rec- ognized housekeeping ...reference genes, RT-qPCR is still the primary approach ...
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Postmenopausal Osteoporosis reference genes for qPCR expression assays
... reference genes belonged to different functional groups, which helped to correct this deficiency from ...reference genes as the output may be ...factor analysis showed more similar values between ...
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Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions
... new genes, SbLAlv9, SbJsn1 and SbSAS1, indicated appropri- ate properties for all the nutrient and light ...reference genes could indeed provide the more reliable genes and improve the assurance of ...
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Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR
... both genes are involved in ubiquitination mechanisms could, a priori, indicate a possible co-regulation, with close results in the rankings, reducing the reliability of our ...coregulated genes for geNorm, ...
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Transcriptome-based identification of the optimal reference CHO genes for normalisation of qPCR data
... any qPCR study in CHO cells To both i) further confirm that a universal set of reference genes can be utilized generically for qPCR experiments in CHO cells, and ii) identify specific, optimal ...
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Reference genes for normalization of qPCR assays in sugarcane plants under water deficit
... affect qPCR performance and produce no reliable results [7, 8, 10, ...reference genes for qPCR analyses, different mathematical algorithms have been proposed, such as NormFinder [11], GeNorm [10], ...
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Validation of miRNA genes suitable as Reference genes in qPCR analyses of miRNA gene expression in Atlantic salmon (Salmo salar)
... miRNA genes that still seemed to be good choices as reference genes in Salmo salar were compared regarding their stability in fifteen tissue samples retrieved from deep se- quencing analysis of small ...
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Validation of miRNA genes suitable as reference genes in qPCR analyses of miRNA gene expression in Atlantic salmon (Salmo salar)
... miRNA genes that still seemed to be good choices as reference genes in Salmo salar were compared regarding their stability in fifteen tissue samples retrieved from deep se- quencing analysis of small ...
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