Genotype MTBDRplus assay
EVALUATION OF THE GENOTYPE MTBDRPLUS ASSAY FOR RIFAMPIN AND ISONIAZID SUSCEPTIBILITY TESTING OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN SPUTUM SPECIME
... Genotype MTBDRplus assay was also performed on mycobacterial cultures according to manufacturer’s (Hain Lifescience) instructions. Briefly, genomic DNA was extracted from bacterial culture by ...
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GenoType MTBDRplus Assay for Molecular Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Strains and Clinical Samples
... the GenoType MTBDR assay, the GenoType MTBDRplus assay, that covers more mutations has recently been ...The GenoType MTBDRplus assay targets the mutations included ...
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Evaluation of the GenoType MTBDRplus Assay for Rifampin and Isoniazid Susceptibility Testing of Mycobacterium tuberculosis Strains and Clinical Specimens
... the GenoType MTBDRplus assay is designed to detect mutations in the regulatory region of ...new assay directly to specimens was shown, since 71 of 72 results for smear-positive sputa and all ...
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Diagnostic accuracy and usefulness of the Genotype MTBDRplus assay in diagnosing multidrug resistant tuberculosis in Cameroon: a cross sectional study
... The emergence of drug-resistant tuberculosis, espe- cially multidrug-resistant tuberculosis (MDR-TB), threatens TB control efforts in most countries including Cameroon. MDR-TB is caused by strains of Mycobac- terium ...
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Validation of the GenoType® MTBDRplus assay for detection of MDR TB in a public health laboratory in Thailand
... This assay has demonstrated good performance and a rapid time to results, making this a promising tool to accelerate MDR-TB diagnosis and improve MDR-TB ...the GenoType ® MDRplus ...
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Validation of the GenoType®MTBDRplus assay for diagnosis of multidrug resistant tuberculosis in South Vietnam
... Beijing genotype was defined by spoligotyping as any isolate without Direct Repeat spacers 1-34 and the presence of ≥3 of the spacers 35-43 ...Vietnam genotype (EAI- VNM) that belongs to the East African ...
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Utility of GenoType MTBDR Plus Assay for Direct Detection and Drug Susceptibility Testing of Mycobacterium tuberculosis from Probable Tuberculous Meningitis Patients
... The GenoType MTBDRplus assay thus proved to be a rapid, easy to perform test for diagnosing TBM in probable TBM ...The assay had much better sensitivity and diagnostic accuracy than microscopy ...
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Drug resistance mutations and heteroresistance detected using the GenoType MTBDRplusassay and their implication for treatment outcomes in patients from Mumbai, India
... The GenoType MTBDRplus assay was used to determine mutations associated with isoniazid and rifampicin resistance and their correlation with treatment ...
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Performance of MTBDRplus assay in detecting multidrug resistant tuberculosis at hospital level
... endorsed Genotype ® MTBDRplus assay for rapid screening of patients at risk of MDR-TB ...probe assay identifies ...the assay independently or parallel with phenotypic DST ...The ...
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Original Article Diagnostic performance of GenoType MTBDR plus on culture specimens in smear-negative retreatment tuberculosis patients
... the GenoType MTBDRplus assay has high sensitivity for the rapid detection of INH- and RIF-resistant ...of GenoType MTBDRplus assay in retreatment ...
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Suitability of Xpert MTB/RIF and Genotype MTBDRplus for Patient Selection for a Tuberculosis Clinical Trial
... the GenoType MTBDRplus assay (MTBDR; Hain Lifescience GmbH, Nehren, Germany), for which mycobacterial DNA is amplified and reversely hy- bridized to detect Mycobacterium tuberculosis complex and ...
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Evaluation of the New GenoType Mycobacterium Assay for Identification of Mycobacterial Species
... The 197 strains used to test the DNA probe system included both clinical isolates from our laboratory collections and reference strains from various in- ternational collections and covered a very wide spectrum of ...
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First Evaluation of an Improved Assay for Molecular Genetic Detection of Tuberculosis as Well as Rifampin and Isoniazid Resistances
... Twenty-two probes for the characterization of the tested strain are located on the DNA strip. TUB represents an MTBC-specific region of the 23S rRNA gene. This zone hybridizes with amplicons generated from all MTBC ...
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Performance Comparison of the Versant HCV Genotype 2 0 Assay (LiPA) and the Abbott Realtime HCV Genotype II Assay for Detecting Hepatitis C Virus Genotype 6
... identify genotype 1 to 5 because a well-characterized set of polymorphisms in this region can predict genotypes well (27, 33, ...the genotype 6 samples could not be genotyped accurately using only the 5= ...
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Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
... commercial assay with good statistical ...GQ-PCR assay and that obtained by direct sequencing (Table ...a genotype-specific PCR assay to verify the genotyping ...PCR assay is highly ...
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Evaluation of the GenoType MTBDR<em>plus</em> and MTBDR<em>sl</em> for the detection of drug-resistant <em>Mycobacterium tuberculosis</em> on isolates from Beijing, China
... MTBDRsl assay for detecting EMB resistance, this assay can be used neither for detecting nor for ruling out EMB resis- tance accurately, and clinicians should use sequencing-based methods for identifying ...
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Genotype MTBDRplus for Direct Detection of Mycobacterium tuberculosis and Drug Resistance in Strains from Gold Miners in South Africa
... the MTBDRplus test was challenging to perform and interpret when run directly on all submitted sputum samples, as approximately 12% of tests could not be interpreted on initial testing and had to be ...the ...
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Improvement of Hepatitis C Virus (HCV) Genotype Determination with the New Version of the INNO LiPA HCV Assay
... for genotype 2 ...HCV assay is also recom- mended by the manufacturer, this procedure is extensively adopted and corresponds to the main routine practice that was evaluated ...
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Genotypic distribution of hepatitis C virus in different regions of Thailand
... genotyping assay could not discriminate between genotype 1b and genotype 6 group variants because of nucleotide sequence homology at the position of probe ...Because genotype 6 group variants ...
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Genetic Determinants of Drug Resistant Tuberculosis among HIV Infected Patients in Nigeria
... for GenoType MTBDRplus testing (n ⴝ 415); mutations were confirmed through ...Finally, GenoType MTBDRplus correctly identified the drug-resistant samples compared to sequencing in ...
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