Gradient centrifugation
Inter center variation in the efficiency of sperm DNA damage reduction following density gradient centrifugation
... We would, therefore, contend that if SDF is to be used as a predictive measure of pregnancy success, then there needs be close attention given to standardizing the spe- cific processes and conditions of ART that are used ...
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Effect of combined density gradient centrifugation on X- and Y- sperm separation and chromatin integrity
... study, centrifugation with PureSperm gradients led to a slight but nonsignificant enrichment in ...albumin gradient centrifugation, we obtained a slight but nonsignificant enrichment of Y- ...
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Solid phase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation test
... Solid-Phase Radioimmunoassay of Rubella Virus Immunoglobulin M Antibodies: Comparison with Sucrose Density Gradient Centrifugation Test OLLI H.. VILJANEN, AND KAISA GRANFORS Departments [r] ...
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Density Gradient Centrifugation Studies on Lymphocytic Choriomeningitis Virus and on Viral Ribonucleic Acid
... 414-420 Copyright © 1970 American Society for Microbiology Density Gradient Centrifugation Studies on Lymphocytic Choriomeningitis Virus and on Viral Ribonucleic Acid Received for public[r] ...
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Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial
... density gradient and swim up methods and found that sperm preparation by density gradient using Percoll re- sulted in higher percentage of DNA damage compared to those separated by swim-up although both ...
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Density Gradient Centrifugation of Rubella Virus
... As EDTA can be seen, the virus obtained from the heavier 1.18 g/cm3 band after 18 hr of centrifugation was B Virus centrifuged in sucrose found on recentrifugation to band at the lighter[r] ...
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Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation
... By using rate-zonal centrifugation followed by tional Institutes of Health, USPHS, Minimum Reisopycnic banding, we previously demonstrated quirements: Influenza Virus Vaccines, Types A a[r] ...
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Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
... Sperm cells (3 × 10 6 ) were washed twice with 9% (v/v) sucrose and solubilized with 250 μl of DeStreak® rehy- dration solution (GE Healthcare, Uppsala, Sweden) with the addition of 1% (v/v) IPG 3–11 solution (GE ...
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Fractionation of Eastern Equine Encephalitis Virus by Density Gradient Centrifugation in CsCl
... It was also recently reported that EEE virus, in the form of a 20% mouse brain suspension, banded at 1.21 to 1.23 g/cm3 in CsCl and that no hemagglutinin was detected in the upper region[r] ...
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Polyacrylamide gel electrophoresis of visna virus polypeptides isolated by agarose gel chromatography.
... When virions were pretreated with NP-40 followed by preliminary fractionation on potassium tartrate gradient centrifugation to separate the virus core heavy fraction from other substruct[r] ...
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Flock House Virus RNA Replicates on Outer Mitochondrial Membranes in Drosophila Cells
... Nycodenz gradient centrifugation of FHV-infected DL-1 lysates to examine the flotation behavior of protein A ...Nycodenz gradient and centrifuged to ...equal-volume gradient fractions by ...
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Methylation of high-molecular-weight subunit RNA of feline leukemia virus.
... It was found that if the aqueous sucrose gradient centrifugation step for the isolation of native 50- 60S FeLV-R RNA was omitted i.e., if total viral RNA was applied directly to the Me2S[r] ...
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Bacteriophages of Rhodopseudomonas spheroides: Isolation and Characterization of a Rhodopseudomonas spheroides Bacteriophage
... The molecular weight of RS1 DNA was determined employing two methods: i from the sedimentation coefficient derived from sucrose gradient centrifugation, as described by Freifelder 6, by [r] ...
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A deletion mutant of L-A double-stranded RNA replicates like M1 double-stranded RNA.
... Ethidium bromide-stained agarose gel A and autoradiogram B of the dsRNAs contained in purified VLPs from strain RE455 by cesium chloride density gradient centrifugation and the products [r] ...
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Cell-associated West Nile flavivirus is covered with E+pre-M protein heterodimers which are destroyed and reorganized by proteolytic cleavage during virus release.
... When such virus preparations were analyzed immediately after preparation by the gradient centrifugation procedure without prior storage in the frozen state, this virus gave the same patt[r] ...
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Biochemical and morphological characterization of mycobacteriophage R1.
... High-titer phage stock purified by cesium chloride gradient centrifugation was utilized for lipid extraction.. The lipid fraction was purified by repeated extractions with chloroformmeth[r] ...
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Structure and chemical-physical characteristics of lactate dehydrogenase-elevating virus and its RNA.
... Two strains of LDV exhibited a density of 1.13 g/cm3 in isopycnic sucrose density gradient centrifugation whether propagated in primary cultures of the various mouse tissues or isolated [r] ...
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Synthesis and Intracellular Localization of Vaccinia Virus Deoxyribonucleic Acid-dependent Ribonucleic Acid Polymerase
... All of these different states of viral DNA can be resolved by 25 to 40% sucrose gradient centrifugation of cytoplasmic fractions from infected cells prepared in the absence of Mg++ at la[r] ...
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A COST-SAVING TECHNIQUE OF HUMAN SOMATIC LIVER STEM CELLS ISOLATION FOR EX VIVO PROPAGATION AND CHARACTERIZATION
... Aim: The study of stem cell biology provides insight into mechanisms that regulate tissue maintenance in normal disease status. With an impressive rate of epithelial renewal driven by a pool of multi potent stem cells ...
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Reovirus-directed Ribonucleic Acid Synthesis in Infected L Cells
... Reovirus was purified from infected cells by genetron treatment and CsCl equilibrium density gradient centrifugation, and double-stranded RNA was extracted from virus with phenol 33.. Be[r] ...
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