gyrB Gene.
Phylogenetic Analysis of Salmonella, Shigella, and Escherichia coli Strains on the Basis of the gyrB Gene Sequence
... of gyrB genes from clinical ...the gyrB nucleotide sequences, adjusted to 1,171 bases, was done by the computer program ...the gyrB gene ...identical gyrB sequences, which were isolated ...
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Use of 16S rRNA, 23S rRNA, and gyrB Gene Sequence Analysis To Determine Phylogenetic Relationships of Bacillus cereus Group Microorganisms
... Grouping of microorganisms in the B. cereus group accord- ing to gyrB gene sequences. Recently, a set of parallel 16S rRNA and partial (more then 60% of the whole gene length) gyrB sequences ...
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Simultaneous Detection of Streptococcus pneumoniae, S mitis, and S oralis by a Novel Multiplex PCR Assay Targeting the gyrB Gene
... Optimization of the multiplex PCR assay.Since mPCR protocols have been developed for the detection of S. pneumoniae and other respiratory pathogens, including S. pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, ...
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High Frequency of Acinetobacter soli among Acinetobacter Isolates Causing Bacteremia at a Tertiary Hospital in Japan
... A. baumannii generally represents the most clinically impor- tant and frequently isolated of the Acinetobacter spp. (2). How- ever, according to a recent report from Norway, Acinetobacter nosocomialis was the most ...
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Alternations in DNA gyrase genes in low-level fluoroquinolone-resistant <em>Moraxella catarrhalis</em> strains isolated in Poland
... and gyrB) and topoi- somerase (parC and parE) genes were determined by polymerase chain reaction and ...and gyrB genes. In gyrA gene, there occurred mutation G412C as well as four silent transition ...
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gyrB Analysis as a Tool for Identifying Nocardia Species and Exploring Their Phylogeny
... and gyrB were com- pared, the strains classified as ...the gyrB-based ...of gyrB (Fig. 2). Changes in GyrB showed that ⬎ 70% of the SNPs were silent, above all in the strains of ...IFM10152 ...
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Evaluation of a Low Density Hydrogel Microarray Technique for Mycobacterial Species Identification
... selected gyrB se- quences and corresponding to different species and subspecies of the My- cobacterium ...the gyrB gene (positions 6332 to 6679, Gen- Bank accession ...the gyrB start site (see ...
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Acinetobacter baylyi as a Pathogen for Opportunistic Infection
... rRNA gene (13), 16S-23S rRNA in- tergenic spacer (ITS) (6), recA gene (15), rpoB and flanking spacers (16), and gyrB gene (27), also allows identification of Acinetobacter ...rRNA gene ...
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Structural differences in gut bacteria communities in developmental stages of natural populations of Lutzomyia evansi from Colombia's Caribbean coast
... Methods: Sand flies were collected from two locations (peri-urban and jungle biotype) in the Department of Sucre (Caribbean coast of Colombia). A total of 752 Lutzomyia evansi intestines were dissected. In this study, ...
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Detection of second line drug resistance in Mycobacterium tuberculosis using oligonucleotide microarrays
... gyrA gene), and two isolates had mutations in the gyrB gene only (D500H, and ...in gyrB gene was resistant to OFX and sensitive to LVX and MFX; however, the role of this mutation in FQ ...
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Association of mutation patterns in gyrA/B genes and ofloxacin resistance levels in Mycobacterium tuberculosisisolates from East China in 2009
... gyrA gene [2,10-15], and that a small number have mutations in the gyrB gene ...gyrA/ gyrB of MTB to determine the drug susceptibility to FQs ...
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SPECIFIC GYRB SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATED FROM SPUTUM OF PULMONARY TUBERCULOSIS PATIENTS IN INDONESIA
... and gyrB gene, only in ...specific gene expression. 19 The high validity of gyrB sequences as taxonomic marker was evaluated mainly: the rate of their base substitutions and the consistency of ...
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Use of an Oligonucleotide Array for Laboratory Diagnosis of Bacteria Responsible for Acute Upper Respiratory Infections
... of gyrB/parE genes. Partial gyrB/parE sequences were obtained from the EMBL public sequence database ...300-bp gyrB/parE gene fragment of interest was confirmed either by direct sequencing or ...
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Rapid Diagnosis of Extensively Drug Resistant Tuberculosis by Use of a Reverse Line Blot Hybridization Assay
... Designing specific sequence probes for fluoroquinolones and second-line ami- noglycosides. The initial 150 XDR-TB isolates were amplified as single targets and sequenced (1). On the basis of the mutations, specific amino ...
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High Proportion of Fluoroquinolone Resistant Mycobacterium tuberculosis Isolates with Novel Gyrase Polymorphisms and a gyrA Region Associated with Fluoroquinolone Susceptibility
... There were some limitations of this study. First, there were relatively few fluoroquinolone-resistant M. tuberculosis isolates. However, almost half of the resistant isolates did not have previ- ously described ...
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GyrB Polymorphisms Accurately Assign Invasive Viridans Group Streptococcal Species
... rRNA gene sequencing, are clinically practical but not sufficiently accurate for VGS species-level iden- ...the gene encoding DNA gyrase subunit B (GyrB) for VGS species-level ...internal ...
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Real Time PCR Assay for Detection of Fluoroquinolone Resistance Associated with grlA Mutations in Staphylococcus aureus
... and gyrB, encoding the B subunits of the DNA topoisomerase IV and DNA gyrase, respectively, will usually generate full resistance to new fluoroquinolones and an increased level of ciprofloxacin resistance ...a ...
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Detection and Molecular Characterization of Mycobacterium microti Isolates in Wild Boar from Northern Italy
... was identified by gyrB RFLP analysis; however, the spoligotype profiles were not always readable. To exclude PCR amplification problems, M. microti DNA was quantified by the Artus M. tuber- culosis PCR kit ...
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Phylogeny and Identification of Nocardia Species on the Basis of Multilocus Sequence Analysis
... and challenging group as the Nocardia. Although DDH has long been held to be the gold standard for prokaryotic species demarcation, MLSA may soon supplant DDH to become the primary measure of bacterial diversity and ...
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Moxifloxacin resistance in the F15/LAM4/KZN extensively drug-resistant strain of <em>Mycobacterium tuberculosis</em>
... gyrA gene in a selection of clinical isolates in KZN, South ...gyrA gene. The QRDR of the gyrA gene consists of a short region, coding for amino acids ...and gyrB genes, as well as flanking ...
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