hairpin RNA
Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids
... induce RNA silencing and protect plants against viruses, including PVY, transmitted mechanically ...by hairpin RNA was effective against inoculation by aphids of non-persistently transmitted viruses, ...
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Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA
... Subacute sclerosing panencephalitis (SSPE) is a demyelinating central nervous system disease caused by a persistent measles virus (MV) infection of neurons and glial cells. There is still no specific therapy available, ...
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Selective gene silencing by viral delivery of short hairpin RNA
... The use of retroviral vectors for efficiently introducing shRNA expression cassettes into target cells has been exploited for many years now. Retroviruses were among the first vectors used as transfer vehicles for ...
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Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA
... pregenomic RNA, reverse transcription template for synthesizing HBV DNA replication ...within RNA-protein com- plexes, perhaps polyribosome complexes on the rough endoplasmic ...
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RNA Editing in Hepatitis Delta Virus Genotype III Requires a Branched Double-Hairpin RNA Structure
... double- hairpin structures are formed are ...nascent RNA transcripts will follow folding path- ways that are dominated by local interactions to arrive at struc- tures that are similar in overall energy but ...
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Trans inhibition of HIV 1 by a long hairpin RNA expressed within the viral genome
... Nef represents a pathogenicity factor that disorders adap- tive immunity by down regulating CD4 and MHC-1 receptors, by inhibiting T-cell chemotaxis, and by induc- ing apoptosis in bystander T-cells, and hence plays a ...
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Short hairpin RNA targeting 2B gene of coxsackievirus B3 exhibits potential antiviral effects both in vitro and in vivo
... study, RNA interference was employed to tar- get the CVB3 protein 2B region (3753-3771), which is a highly conserved region in most enteroviruses and there- fore is an attractive therapeutic ...genomic RNA, ...
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<p>Pre-mRNA Processing Factor 8 Accelerates the Progression of Hepatocellular Carcinoma by Regulating the PI3K/Akt Pathway</p>
... short hairpin RNA method, and the role of Prp8 on cell proliferation and migration was examined by Cell Counting Kit-8, wound healing assay and Transwell assays following knockdown in HCC cells, and ...
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Depletion of Kcnq1ot1 non coding RNA does not affect imprinting maintenance in stem cells
... employed RNA interference-based technology to deplete Kcnq1ot1 ...short hairpin RNA-mediated depletion in embryonic stem, trophoblast stem and extra-embryonic endoderm stem cells had no observable ...
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Tetrahymena thermophila JMJD3 Homolog Regulates H3K27 Methylation and Nuclear Differentiation
... a hairpin RNA-knockdown approach, we showed that JMJ1 expression is critical for progeny production during conjugation and the dem- ethylation of H3K27me3 in late stages of ...
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Enhancement of Hepatitis B Virus Replication by Androgen and Its Receptor in Mice
... and RNA levels in mice before puberty, but its effect on HBV after puberty was apparent, with HBV replicating approximately twice more efficiently in male mice than in female mice whether or not HBx was ...and ...
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Silencing B7-H1 enhances the anti-tumor effect of bladder cancer antigen-loaded dendritic cell vaccine in vitro
... short hairpin RNA (shRNA) sequence aimed at B7-H1 and then infected DCs to investigate whether B7-H1 knockdown could augment the immune function of DCs and further elicit a more potent anti-tumor immune ...
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Original Article A RTK-based functional RNAi screen reveals determinants of PTX-3 expression
... short hairpin RNA (shRNA) library representing the full comple- ment of 56 human RTKs (Table 1) for genes whose inhibition could impair PTX-3 expression in U937 ...
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Polycomb recruitment attenuates retinoic acid induced transcription of the bivalent NR2F1 gene
... short hairpin RNA (shRNA) technology enhanced the RA-associated transcription of Nr2F1, Nr2F2, Meis1, Sox9 and BMP2, but had no effect on the Hoxa5, Hoxa1, Cyp26a1, Cyp26b1 and RARb 2 transcript levels in ...
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SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma
... Purpose: Cytoskeleton is critical for carcinoma cell proliferation, migration, and invasion. Sad-1 and UNC-84 domain containing 1 (SUN1) is one of the core linkers of nucleoskeleton and cytoskeleton. However, the ...
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Efficient Herpes Simplex Virus 1 Replication Requires Cellular ATR Pathway Proteins
... Herpes simplex virus 1 (HSV-1) is a double-stranded DNA virus that replicates in the nucleus of the host cell and is known to interact with several components of the cellular DNA-damage-signaling machinery. We have ...
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Review on: Short Hairpin Ribonucleic Acid (RNA)
... double-stranded RNA (dsRNA) was at least ten-fold or perhaps a hundred fold more potent as a silence trigger than was sense or antisense ...double-stranded RNA contamination in those samples ...
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Opening of the TAR hairpin in the HIV 1 genome causes aberrant RNA dimerization and packaging
... vitro RNA dimerization assays, we previously observed that opening of 5′ TAR (A 5 and B 5 mutations) caused increased dimerization of leader RNA transcripts, whereas the AB 5 transcripts showed the ...
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An Internally Located RNA Hairpin Enhances Replication of Tomato Bushy Stunt Virus RNAs
... or RNA-RNA interactions (21, 43) have been implicated in similar proofreading functions in other plus strand RNA ...of RNA syn- thesis in a variety of viral template ...
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Gene therapy approaches to functional cure and protection of hematopoietic potential in HIV infection
... enables RNA-based gene silencing, including the previously characterized small interfering RNA (siRNA) called PromA ...short RNA sequence specific for the two NF-κB binding sites in the HIV LTR U3 ...
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