hammerhead ribozymes
Intracellular cleavage of hepatitis C virus RNA and inhibition of viral protein translation by hammerhead ribozymes
... of hammerhead ribozymes either by RNA itself or by expression from DNA expression vectors into the HCV-infected hepatocytes can substantially inhibit HCV translational ...
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Retrozymes are a unique family of non autonomous retrotransposons with hammerhead ribozymes that propagate in plants through circular RNAs
... that hammerhead ribozyme sequences in plant genomes form part of a new family of small non-autonomous retrotransposons with hammerhead ribozymes, referred to as ...a hammerhead ribozyme that ...
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Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome
... several hammerhead ribozymes for suppressing DENV in lentivirus-transduced mosquito cells in an attempt to mimic the transgenic use of these effector molecules in ...these ribozymes as a fusion RNA ...
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A 451-nucleotide circular RNA from cherry with hammerhead ribozymes in its strands of both polarities.
... form hammerhead structures similar to those found previously in other plant and animal small ...the hammerhead structures, which comprise approximately one-fourth of this RNA, are found opposite each other, ...
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The analysis of chimeric ribozymes in plants
... tRNARz12 expression in transgenic plants: a comparison with pAtRNARz12 transfected plant cells DISCUSSION 80 81 CHAPTER 7 THE APPLICATION OF HAMMERHEAD RIBOZYMES FOR TARGETED IN VIVO GEN[r] ...
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Eggplant Latent Viroid, the Candidate Type Species for a New Genus within the Family Avsunviroidae (Hammerhead Viroids)
... the hammerhead structures (14, 20, 28), and a radioactive probe obtained by PCR from this insert hybridized with the ELVd circular and linear ...through hammerhead ri- bozymes (12, ...
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Expressing Active Ribozymes in Cells
... of ribozymes do not have identical ...with hammerhead ribozymes and short substrate molecules has shown that above a certain stability threshold of the ribozyme/substrate complex, usually reached ...
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Developing Tools and Techniques for Expanding the Synthetic Biology Toolkit.
... that hammerhead ribozymes are associated with varying cleavage activities across different model systems ...structures, ribozymes can form complex 3D structures ...
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Elimination of hepatitis C virus RNA in infected human hepatocytes by adenovirus-mediated expression of ribozymes.
... six ribozymes with potent ability to degrade HCV from experi- mental cell lines and from liver cells isolated from two patients with chronic HCV ...expressed ribozymes which cleave conserved regions of both ...
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RIBOZYMES: NUCLEIC ACID ENZYMES WITH POTENTIAL PHARMACEUTICAL APPLICATIONS - A REVIEW
... catalytic hammerhead between them are then designed based on the target sequence surrounding the third nucleotide (X) of the target ...design ribozymes against selected RNA targets has expanded their ...
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Adenovirus-mediated expression of ribozymes in mice.
... different ribozymes for catalytic self-cleavage exist in nature, the simplest and best characterized are the hammerhead ...fined, hammerhead ribozymes are RNA molecules that hybrid- ize to ...
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RNase footprinting demonstrates antigenomic hepatitis delta virus ribozyme structural rearrangement as a result of self cleavage reaction
... studied ribozymes which exhibit almost equal activity have similar nuclease digestion profiles for the original molecule (Figures 2a and 2f, 3a and 3f, 4a and ...pre-cleaved ribozymes of the LBS family ...
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Factors Governing the Activity In Vivo of Ribozymes Transcribed by RNA Polymerase III
... other ribozymes for suppression of several other genes, keeping in mind that their secondary structures should resemble those of Rz1 through Rz3 ...these ribozymes were found in the cytoplasm after ...
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Kinematics and critical swimming speed of juvenile scalloped hammerhead sharks
... Critical swimming speeds of 11 hammerhead sharks were measured using methods described by Graham et al. (1990). Attempts to acclimate the shark pups in the flume overnight were unsuccessful and resulted in high ...
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Report on the population status of hammerhead sharks in the Galapagos Marine Reserve
... of hammerhead sharks at Darwin Island was estimated using a combination of visual counts with acoustic telemetry (methodology described in Peñaherrera-Palma, ...
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Stock assessment of whaler and hammerhead sharks (Carcharhinidae and Sphyrnidae) in Queensland
... scalloped hammerhead Sphyrna lewini is unknown, because very few mature females of this species have been caught and the proportion that are gravid (pregnant) at any one time is impossible to ...great ...
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Functional repair of a mutant chloride channel using a trans splicing ribozyme
... intron ribozymes have been used to mediate trans-splicing of therapeutically relevant RNA transcripts, but the efficiency of the ribozyme-mediated RNA repair process has not been determined precisely and ...
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Poliovirus Requires a Precise 5′ End for Efficient Positive-Strand RNA Synthesis
... FIG. 1. A poliovirus-specific cis-acting hammerhead ribozyme. (A) Predicted secondary structure of the cis-active hammerhead ribozyme attached to the 5⬘ end of the poliovirus genome. Core residues of the ...
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Engineered RNase P Ribozymes Effectively Inhibit the Infection of Murine Cytomegalovirus in Animals
... with ribozymes R388-AS and ...receiving ribozymes R388-AS and M1-AS was studied and compared to animals receiving PBS only or the control ribozymes C-R388-AS, C-M1-AS, or ...Control ribozymes ...
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Multigene antiviral vectors inhibit diverse human immunodeficiency virus type 1 clades.
... In this study, we have developed retroviral vectors that achieve (i) high expression of the transgenes, (ii) different mechanisms of inhibition of HIV by using fusion molecules of ribozymes and of an RRE decoy, ...
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