HEK 293T
S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T Cells
... Human Reelin containing 5th and 6th repeats (R5-R6), a part of 8 th repeat and the CTR which were amplified from cDNA of K562 and HEPG2 cells and cloned into a mammalian expressional plasmid (pVP22/myc-His). ...
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Transcriptome Analysis Reveals a Signature Profile for Tick Borne Flavivirus Persistence in HEK 293T Cells
... (HEK) 293T cells in vitro results in the death of >95% of infected cells by day ...the HEK 293T cell transcriptome to deep sequencing to identify genes differentially expressed during acute ...
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Development of a Model System for Tick Borne Flavivirus Persistence in HEK 293T Cells
... for HEK 293T cells, observation of the addi- tional bouts of Vero and MA-111 cell death suggests that JEV persistence was not unique to a minor cell population with an inherent ...
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Incorporation of podoplanin into HIV released from HEK 293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC 2
... knock-down cells. For this, the cells were transfected with env-deficient HIV-1 proviral DNA (for augmented bio- safety), the supernatants concentrated by size-exclusion filtration and virions pelleted by centrifugation ...
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A novel two step genome editing strategy with CRISPR Cas9 provides new insights into telomerase action and TERT gene expression
... parental HEK 293T cells ...in HEK 293T cells, it is perhaps not surprising that the introduction of the TERT promoter mutation did not lead to a further in- crease of TERT ...
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Accuracy estimation of foamy virus genome copying
... in HEK 293T cells by Western blotting ...in 293T cells were about 25% of the amount detected in PBMC ...the HEK 293T cells used in our laboratory, we also analyzed HEK ...
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The Tyro3 Receptor Kinase Axl Enhances Macropinocytosis of Zaire Ebolavirus
... Vero, HEK 293T, or VeroE6 cells that do not require Axl expression for filovirus infection, which we call Axl-independent cells (6, 22, 59, 81); similar studies in Axl- dependent cells have not been ...
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Foamy Virus Nuclear RNA Export Is Distinct from That of Other Retroviruses
... of HEK 293T cells together with pcHSRV2 and ...levels. 293T cells were cotransfected with pcHSRV2 and increasing amounts of pHt-ANP32B or with 2 g of the pHt vector as a ...
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Sensing of HIV-1 Entry Triggers a Type I Interferon Response in Human Primary Macrophages
... microbeads (Miltenyi) and differentiated into macrophages for 7 days in RPMI (Gibco, Life Technologies) supplemented with 5% fetal calf serum (FCS; BioWest), 5% human serum AB (Sigma), penicillin- streptomycin (Gibco), ...
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Cryptosporidium parvum Long-Chain Fatty Acid Elongase
... Total fatty acid elongation activity was measured in isolated membrane fractions of HEK-293T cells transfected with CpLCE1 and compared with that in HEK-293T cells trans- fected with the[r] ...
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The HIV 1 late domain 2 S40A polymorphism in antiretroviral (or ART) exposed individuals influences protease inhibitor susceptibility
... in HEK 293T cells, producing two MBP-containing prod- ucts with residual amount of unprocessed precursor (panel C, top, lane ...fected HEK 293T cells [32, 33, 36] and ...
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Original Article DSTYK knockout affects the architecture of F-actin cytoskeleton in mouse renal proximal convoluted tubules
... Among the list of possible DSTYK interacting proteins, Profilin 2 is of particular interest because Profilins are small actin binding pro- teins which play an important role in F-actin polymerization and regulate the ...
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The G-Patch Domain of Mason-Pfizer Monkey Virus Is a Part of Reverse Transcriptase
... Western blot analysis of the concentrated M-PMV stock using anti-GPD antibody revealed the presence of not only PR17 but also a protein with an approximate molecular mass of 50 kDa (p50-GPD). This p50-GPD protein was ...
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Original Article Expression of flotillin-2 in human non-small cell lung cancer and its correlation with tumor progression and patient survival
... and HEK-293T) and NSCLC tissues was significantly higher com- pared to that in normal bronchial epithelial cell line 16HBE and adjacent non-tumor ...
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DiDonato_2019.pdf
... Given that no differences in phospho-NPM1 levels were observed in HEK-293T cells or BJAB cells, the specificity of the anti phospho-NPM1 antibody to detect only phosphorylated NPM1 and not total NPM1 was ...
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The HDAC6/APOBEC3G complex regulates HIV 1 infectiveness by inducing Vif autophagic degradation
... in HEK 293T cells overexpressing wt-HDAC6, where Vif is degraded and A3G subsequently ...in HEK 293T cells overexpressing HDAC6- Δ BUZ was ...
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Role of the Phosphatidylserine Receptor TIM-1 in Enveloped-Virus Entry
... This study demonstrates that TIM-1 enhances infection of a vari- ety of unrelated enveloped viruses by binding to virion-associated PtdSer, leading to virus internalization. The virus families de- scribed here include ...
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A Map of the Arenavirus Nucleoprotein-Host Protein Interactome Reveals that Junín Virus Selectively Impairs the Antiviral Activity of Double-Stranded RNA-Activated Protein Kinase (PKR)
... viruses. HEK 293T/17 cells (CRL-11268) and A549 cells (CCL-185) were procured from the American Type Culture Collection (ATCC, Manassas, ...CA). HEK 293T/17 cells were cultured in Dulbecco’s ...
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Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis
... OGT is the potential direct target gene of miR‑7‑5p We found five downstream targets of miR-7-5p on Star- base database after strictly limiting binding conditions (Clip data:high stringency ≥ 3; Degradome data: medium ...
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Improved fusion protein expression of EGFP via the mutation of both Kozak and the initial ATG codon
... products. These results revealed that RNA samples from the transfected HEK 293T cells were not contaminated by the transfected recombinant plasmids (Fig. 4, lanes 1, 3, 5 and 7). RT-PCR with FP-P and RP2 as ...
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