hepatitis C virus nonstructural protein 5A
A Conserved Tandem Cyclophilin-Binding Site in Hepatitis C Virus Nonstructural Protein 5A Regulates Alisporivir Susceptibility
... The C-terminal half of NS5A that contains the tandem CyPA-binding site belongs to a class of proteins that are designated intrinsically disordered proteins (IDPs), also known as natively unfolded proteins or ...
12
Inhibition of Intrahepatic Gamma Interferon Production by Hepatitis C Virus Nonstructural Protein 5A in Transgenic Mice
... Hepatitis C virus (HCV) utilizes strategies to suppress or evade the host immune response for establishment of persistent ...HCV nonstructural protein 5A (NS5A) impairs tumor ...
7
Interaction of Hepatitis C Virus Nonstructural Protein 5A with Core Protein Is Critical for the Production of Infectious Virus Particles
... fever virus NS2A block production of infectious virus, and this perturbation can be released by a suppressor mutation in NS3 (25), while the hydrophobic residues of Kunjin virus NS2A required for ...
13
Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK/SREBP-1c signaling
... To further confirm our results and understand whether increased p-AMPK attenuates NS5A-induced lipogen- esis, Hepa1–6 and HepG2 cells were co-transfected with NS5A-, AMPKα1- and AMPKα2-expressing plasmids. As expected, ...
13
Characterization of a Threonine-Rich Cluster in Hepatitis C Virus Nonstructural Protein 5A and Its Contribution to Hyperphosphorylation
... a minor subspecies of p58, since its abundance only marginally changed, even in cases where total p58 levels were dramatically reduced (e.g., S229A and S232A). Second, pT242 synthesis is regulated by C-terminal ...
24
Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
... We pursued a more systematic, sensitive, quantitative anal- ysis of the cross-linking experiment using domain I-plus. We observed a time (data not shown) and glutaraldehyde concen- tration dependence to the cross-linking ...
12
Hepatitis C Virus Nonstructural Protein 5A Modulates the Toll-Like Receptor-MyD88-Dependent Signaling Pathway in Macrophage Cell Lines
... cells per well, washed twice with PBS, fixed with PBS containing 4% paraformaldehyde at 18 h of cultivation, and permeabilized with PBS con- taining 0.5% Triton X-100 at 15 min. The cells were then incubated at ...
14
Sequential S232/S235/S238 Phosphorylation of the Hepatitis C Virus Nonstructural Protein 5A
... S229. Protein kinases, including polo-like kinase 1 (38), calmodulin-dependent kinase II (35), and GSK3  (22) reported to affect NS5A phosphorylation, may be recruited for S229 ...
14
Hepatitis C Virus Nonstructural 5A Protein and Interferon Resistance: a New Model for Testing the Reliability of Mutational Analyses
... NS5A protein were ...NS5A protein was predicted (39, ...NS5A protein, should be of minor relevance, whereas noncon- served amino acid mutations and especially mutations for amino acids located on the ...
12
Regulation of Hepatitis C Virus Replication by Nuclear Translocation of Nonstructural 5A Protein and Transcriptional Activation of Host Genes
... Naturally occurring NS5A variants are associated with dif- ferent HCV RNA replication efficiencies in vitro. To test the hypothesis that NS5A mutations naturally occurring in vivo could be associated with different ...
18
Nonstructural 5A Protein of Hepatitis C Virus Regulates Soluble Resistance-Related Calcium-Binding Protein Activity for Viral Propagation
... PLK1-mediated phosphorylation of sorcin is increased by NS5A. We next investigated whether phosphorylation of sorcin was also regulated by NS5A protein. For this purpose, HEK293T cells were cotransfected with ...
12
Interferon Resistance of Hepatitis C Virus Genotype 1b: Relationship to Nonstructural 5A Gene Quasispecies Mutations
... the nonstructural 5A (NS5A) protein of hepatitis C virus genotype 1b (HCV-1b) was recently suggested to be the interferon sensitivity-determining region (ISDR), because HCV-1b ...
11
Hepatitis C Virus Nonstructural 5A Protein Induces Interleukin-8, Leading to Partial Inhibition of the Interferon-Induced Antiviral Response
... Plasmids. A full-length NS5A construct obtained from a genotype 1b IFN- nonresponsive patient was cloned into pTRE as described previously (58) and designated pTRE-FL-NS5A-NR. A series of amino- and carboxy-terminal ...
12
Isolation and Characterization of Monoclonal Antibodies That Inhibit Hepatitis C Virus NS3 Protease
... Nonstructural protein 3 (NS3) of hepatitis C virus (HCV) is a multifunctional virus-specific protein that contains serine protease activity in its N-terminal region and ...
9
Protein-Protein Interactions between Hepatitis C Virus Nonstructural Proteins
... In order to confirm the coimmunoprecipitation data for this cell line and to shed light on interactions seen in the other assays that were not observed with Huh-7 cells, we examined the colocalization of individual ...
14
Nonstructural Protein 5A (NS5A) and Human Replication Protein A Increase the Processivity of Hepatitis C Virus NS5B Polymerase Activity In Vitro
... Preparation of RNA rolling-circle template. Briefly, two RNA oligo- nucleotides (74 nucleotides [nt]and 106 nucleotides) were synthesized with T7 RNA polymerase on a parental DNA template primed with a T7 promoter ...
16
Subversion of Cell Signaling Pathways by Hepatitis C Virus Nonstructural 5A Protein via Interaction with Grb2 and P85 Phosphatidylinositol 3-Kinase
... (lanes 1 and 2) were either treated with EGF (lanes 1 to 3, 5, and 6) or left untreated (lane 4), and cell lysates were collected at different time points post-EGF treatment as indicated. Coimmunoprecipitation assays ...
11
Pim Kinase Interacts with Nonstructural 5A Protein and Regulates Hepatitis C Virus Entry
... epatitis C virus (HCV) is a major etiological agent of chronic liver disease, including cirrhosis and hepatocellular carci- noma ...enveloped virus with a positive-sense, single-stranded RNA that ...
14
The C-Terminal α-Helix Domain of Apolipoprotein E Is Required for Interaction with Nonstructural Protein 5A and Assembly of Hepatitis C Virus
... Hepatitis C virus (HCV) remains a major global health problem, chronically infecting approximately 170 million peo- ple worldwide, with severe consequences such as hepatitis, ...
10
Efficient Rescue of Hepatitis C Virus RNA Replication by trans-Complementation with Nonstructural Protein 5A
... Our results for HCV are in line with a recent report by Grassmann and coworkers, who studied the conditions for trans-complementation of the pestivirus BVDV (22). It was found that only mutations in NS5A, and not ...