High Resolution Melting Curve Analysis
Detection of monoclonal immunoglobulin heavy chain gene rearrangement (FR3) in Thai malignant lymphoma by High Resolution Melting curve analysis
... From melting temperature of duplex DNA, 3 samples accounting for ...with high-speed amplification, and Idaho-Technology with rapid and high-resolution melting curve ...
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High Resolution Melting Curve Analysis for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis: a Meta Analysis
... treatment. High-resolution melting curve analysis has been used for the detection of rifampin resistance in Mycobacterium tuberculosis and has shown ...meta- analysis of ...
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Real time RT PCR high resolution melting curve analysis and multiplex RT PCR to detect and differentiate grapevine leafroll associated virus 3 variant groups I, II, III and VI
... consistent melting points per sample, how- ever, significant variation was observed between samples from the same variant ...the high mutation rate of the viral genome [22,23]. An average melting ...
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Classification of Fowl Adenovirus Serotypes by Use of High Resolution Melting Curve Analysis of the Hexon Gene Region
... Diagnosis of FAdV infections can be made from the obser- vation of gross and histopathological changes in the liver and the use of electron microscopy (3, 11) and various serological tests, such as enzyme-linked ...
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Ultrasensitive Detection of Drug Resistant Pandemic 2009 (H1N1) Influenza A Virus by Rare Variant Sensitive High Resolution Melting Curve Analysis
... HRM analysis was used as a foundation to create ...the melting curves of the resulting amplifica- tion products from each well were subjected to HRM ...different melting-curve profiles were ...
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High Resolution Melting Curve Analysis for Rapid Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Clinical Isolates
... HRM curve analysis is simple and highly sensitive and ...very high sensitivities and specificities (94 to 98% and 96 to 100%, respectively) for detection of RIF ...by analysis of HRM ...HRM ...
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Detecting differential allelic expression using high-resolution melting curve analysis: application to the breast cancer susceptibility gene CHEK2
... a high-resolution melting (HRM) analysis approach to perform allele-specific expression measurement in CHEK2 ...DAE analysis was limited by the single- capillary throughput of the HRM ...
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High Resolution Melting Curve Analysis for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis Clinical Isolates
... costly. High-resolution melting (HRM) curve analysis is a simple technique and has been widely used for many ...in melting point (T m ) compared to reference DNA; it does not ...
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Gene Expression and Methylation Pattern in HRK Apoptotic Gene in Myelodysplastic Syndrome
... Sensitive-High Resolution Melting Curve Analysis (MS-HRM) in MDS patients, as well as healthy controls and EpiTect®PCR Control ...
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The Use of High Resolution Melting Analysis for Detection of the CAPN530 Polymorphism in Slovak Simmental Bulls
... a high throughput web application for PCR and sequencing primer ...J., High-resolution melt curve analysis: Initial screening for mutations in BCR-ABL kinase domain, Leuk Res, 2008, 32, ...
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Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
... the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop ...The ...
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High resolution melting analysis for molecular detection of multidrug resistance tuberculosis in Peruvian isolates
... HRM analysis can be employed to detect MDR-TB due to their high sensitivity, specificity and efficiency [20, 22, ...HRM curve analysis as a method for the rapid and accurate detection of ...
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... The high-resolution melting (HRM) assay is a novel and powerful molecular method applied to mutation detection, genotype analysis and species identifica- tion ...temperature, melting ...
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Detection of R882 Mutations in DNMT3A Gene in Acute Myeloid Leukemia: A Method Comparison Study
... establish high resolution melting (HRM) curve analysis as a rapid and sensitive test to identify DNMT3A gene mutations in AML ...
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The mutation profile of JAK2 and CALR in Chinese Han patients with Philadelphia chromosome-negative myeloproliferative neoplasms
... High-resolution melting analysis (HRMA) was used to screen for genetic alternations in JAK2 Exon ...a high mutation detecting sensitivity ...The melting fluorescent data were ...
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Rapid Detection of Rifampicin and Isoniazid Resistant Mycobacterium tuberculosis by High Resolution Melting Analysis
... HRM analysis were performed on the Rotor-Gene 6000 (Corbett Research, Sydney, ...melt curve was generated by heating, using the temperature ranges indicated in Table 1, at increments of ...melt curve ...
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Development of a Rapid High Throughput Method for High Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses
... Melting-curve analysis was performed using the LightCycler 480 gene-scan- ning software module as reported by Wittwer et ...HRM curve data to identify changes in the shape of the curve ...
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Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE PCR)
... ments. Melting curve assays have been described to detect the ...for high volume screening, since the use of agarose gel electrophoresis for detection makes it a time consuming open tube ...melt ...
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Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B ( p15 ) gene
... earlier melting of heteroduplexes relative to ...resulting melting curve is flattened and exhibits a complex melting ...a melting profile that cannot be directly compared with ...
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Multiplex Real Time PCR Melting Curve Assay To Detect Drug Resistant Mutations of Mycobacterium tuberculosis
... relatively high and a real-time PCR instrument with multiple channels is ...by melting curve ...probe melting curve analysis and high-resolution melting ...
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