high-throughput mass spectrometry
High Resolution Genotyping of Campylobacter Species by Use of PCR and High Throughput Mass Spectrometry
... and mass spectrometry base composition ...a high-pH buffer. For optimal calibration of the mass spectrometer, internal mass standards, which bracket the m/z range of the PCR products’ ...
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1 High-throughput direct mass spectrometry-based
... regional brain alterations in the APP/PS1 transgenic model of Alzheimer’s disease by direct infusion mass 308. spectrometry[r] ...
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A SIMPLE, SENSITIVE, SELECTIVE AND HIGH THROUGHPUT LIQUIDCHROMATOGRAPHY/TANDEM MASS SPECTROMETRY (LC/MS/MS)ASSAY OF EXEMESTANEIN HUMAN PLASMA
... (heater gas flow): 60.0 psig; ion spray voltage (ISV): 5500.0V, turbo heater temperature (TEM): 400.0°C; interface heater (Ihe): ON; entrance potential (EP): 9.0V; collisional activated dissociation (CAD): 4 psig and ...
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Towards high throughput and spatiotemporal proteomics : analytical workflows and quantitative label-free mass spectrometry
... 100 to 100,000 µm 3 . 24 It can not be assumed that the cell density or cell sizes will be the same in the heat-shocked cultures as in the control cultures (in fact, we know it will not be, as 42°C is not optimal for the ...
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Two common functional catalase gene polymorphisms (rs1001179 and rs794316) and cancer susceptibility: evidence from 14,942 cancer cases and 43,285 controls
... PCR: polymerase chain reaction; RFLP: restriction fragment length polymorphism; HM L/I MS: high-throughput, matrixassisted, laser desorption/ionization time-of-flight mass spectrometry; [r] ...
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High-throughput profiling of small molecules using mass spectrometry
... Studies in zebrafish larvae are increasingly used for modeling human diseases with the ex- pectation that they will be able to contribute towards bridging the gap between in vitro cell based assays and in vivo mammalian ...
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High throughput discovery of thermo responsive materials using water contact angle measurements and time of flight secondary ion mass spectrometry
... The 4 polymer coupons with the largest measured WCA with a change in temperature were analysed by ToF-SIMS at 2 temperatures to see if any molecular structural changes could be detected at the surface that cause the ...
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Mass spectrometry imaging of cassette dosed drugs for higher throughput pharmacokinetic and biodistribution analysis
... of mass spectra at discrete points on a tissue section, have recently emerged as technologies that have the capability to obtain label-free compound biodistribution, relative abundance and quantitation data by ...
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Simultaneous untargeted and targeted metabolomics profiling of underivatized primary metabolites in sulfur-deficient barley by ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry
... crop), high-throughput methods need to be ...The high polarity and acid–base properties implies time consuming sample preparation ...targeted high performance liquid chro- ...
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High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry
... and mass spectrometry enables amino acids to be analysed significantly faster than by older methods using UV or fluorescent ...the mass detection, the typi- cal cycle time (time from injection to ...
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High Throughput Identification of Bacteria and Yeast by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry in Conventional Medical Microbiology Laboratories
... flight mass spectrometry (MALDI-TOF MS) is suitable for high-throughput and rapid diagnostics at low costs and can be considered an alternative for conventional biochemical and molecular ...
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Nagaraj, Nagarjuna (2010): Developing mass spectrometry towards applications in clinical proteomics. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... tandem mass spectrometry has gained in popularity over the quadrupole mass ...include high sensitivity, fast scan speeds and parallel operation with the Orbitrap or FT ...yields high ...
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Discovering human protein diversity
... First, mass spectrometry is capable of detecting structural protein modifications, and, when coupled to immunoaffinity separations, it can be employed in a high-throughput systematic study of ...
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A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry
... 5600+ mass spectrometer (AB SCIEX) for tandem mass spectrometry with the following parameters: ion spray voltage was set to 2300V, declustering potential of 150 V, curtain gas flow 25, nebuliser gas ...
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A SENSITIVE, HIGH THROUGHPUT ESTIMATION OF METHOTREXATE IN HUMAN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY
... and high throughput high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method has been developed for estimation of methotrexate in human ...The mass ...
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Applications of Mass Spectrometry to Analysis of Prodiginines, Bioactivated Methylenedianiline Intermediates, and Hypoxia Induced Changes in the Zebrafish Skeletal Muscle Proteome
... 45 mass spectrometry earned the reputation for its high sensitivity, broad dynamic range of measurements, rich information obtained from complementary fragmentation techniques like CID, 45, 46 ...
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High mass accuracy analytical applications of Fourier transform ion cyclotron resonance mass spectrometry
... Since then, several studies have reported on the fragmentation patterns obtained when singly charged molecules, such as biomolecules 75 and inorganic complexes, 76 are allowed to interact with electrons, a technique ...
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Advances in high field asymmetric ion mobility spectrometry (FAIMS) analyzers and FAIMS-mass spectrometry interfaces
... FAIMS analyzers may be constructed in any number of configurations, with only three major requirements: 1) A gap between two electrodes, across which a time-dependent asymmetric waveform composed of a high and ...
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Investigating protein structure by means of mass spectrometry
... TOF mass analyser offers the advantage that mass spectra can be acquired on the microsecond time scale whilst mobility separation occurs on the millisecond time ...of mass spectra can thus be ...
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Formation of Mercury(II) Glutathione Conjugates Examined Using High Mass Accuracy Mass Spectrometry
... provided insight into the stoichiometry of binding be- tween Hg(II) and GSH, with particular attention on the dissociation behavior of the conjugates under collision- ally induced dissociation (CID) conditions by tandem ...
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