High-throughput next generation sequencing
High Throughput Next Generation Sequencing of Polioviruses
... Sanger-based sequencing of the viral protein 1 (VP1) capsid region is currently the standard method for PV ...whole-genome sequencing protocols for poliovirus isolates and FTA cards using ...
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High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing
... Analyzer next generation sequencing ...the throughput, which actually allowed an analysis of 31 libraries in three eighth of the capacity of a single Illumina GAII sequencing run (Table ...
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Advances in genetics and molecular breeding of three legume crops of semi-arid tropics using next-generation sequencing and high-throughput genotyping technologies
... in next-generation sequencing (NGS) and high-throughput (HTP) genotyping methods, there is a shift in development of genomic resources including molecular markers in these ...Illumina ...
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Culture-Independent Metagenomic Surveillance of Commercially Available Probiotics with High-Throughput Next-Generation Sequencing
... to next-generation whole-genome sequencing and analyzed by a custom in-house-developed k-mer counting method to validate manufacturer la- bel ...of next-generation sequencing as ...
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Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples.
... (snoRNAs) found in intron regions of the human genome may play an important role in regu- lating gene expression [ 31 , 32 ]. The role of intron sequences in HLA expression and their importance in HSCT have recently ...
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High-Throughput Characterization of Foodborne Pathogens using Next-Generation Sequencing
... minants, which can be transferred to humans through different routes. Pre- vious studies have sought to assess the degree to which AMR livestock- and human-associated Salmonella strains overlap, as well as the spatial ...
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High-Throughput Discovery of Mutations in Tef Semi-Dwarfing Genes by Next-Generation Sequencing Analysis
... Sanger sequencing, including two predicted detrimental mutations in both dw3 homeologues with a potential to improve lodging resistance in tef through further ...that high- throughput ...
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High throughput RNA interference screening using pooled shRNA libraries and next generation sequencing
... Sequencing data analysis Raw images from the Illumina GAIIx were processed using the GApipeline (version 1.4 or higher) and result- ing quality filtered short reads were aligned to the refer- ence shRNA library ...
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A high throughput sequence analysis infrastructure technology investigation framework for the evaluation of next generation sequencing software
... exon sequencing and a recently developed probabilistic disease-gene discovery algorithm to identify a missense variant in NAA10, which encodes the catalytic subunit of the major human amino-terminal ...
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Expanding preconception carrier screening for the Jewish population using high throughput microfluidics technology and next generation sequencing
... broader-scaled sequencing is advantageous in a research setting, or in clinical testing of affected individuals, it still lacks sufficient clinical validity and utility for screen- ing healthy ...with ...
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Accurate, rapid and high-throughput detection of strain-specific polymorphisms in Bacillus anthracis and Yersinia pestis by next-generation sequencing
... the generation of over 50 GB (and counting) of sequence data from a single instrument run, an example being the sequencing by oligonucleotide ligation and detection (SOLiD ™ ) system from Applied ...of ...
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Original Article Application of high-throughput sequencing technologies with target capture/target next-generation sequencing in diagnosis of neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD)
... mutation (c.128_134del) and the other was a 23 bp insertion mutation (c.1663_1664ins). These mutation sites were confirmed by Sanger sequencing, the coincidence rate respectively is 100%. This research suggested ...
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Next generation sequencing
... parallel sequencing is not only an end, but also a means for performing experiments that may answer questions that could not even be asked ...parallel sequencing methods is akin to the revolution fostered ...
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Next Generation Sequencing
... Next Generation Sequencing DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection Over the past ...
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Next Generation Sequencing
... DNA Sequencing – the next generation • NGS refers to non-‐Sanger-‐based high-‐ throughput DNA sequencing technologies... DNA Sequencing – the next generation..[r] ...
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Evaluation of analytical protocols of alignment mapping tools using high throughput next-generation genome sequencing data
... [7] Niedringhaus TP, Milanova D, Kerby MB, Snyder MP, Barron AE. Landscape of next-generation sequencing technologies. Anal Chem. 2011;83:4327–41. [8] Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, ...
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Next generation sequencing in thyroid cancer
... consistently and sustainably existing from both tumor development and during tumor progression. In thyroid cancer, BRAF V600E is the most common and the earliest genetic event in PTC [42, 43], and it appears to be a good ...
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Next-generation DNA sequencing techniques
... The high throughput of next-generation sequencing technology, rapidly producing huge numbers of short sequencing reads, made possible the analysis of a complex sample containing ...
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High-Throughput Sequencing and Metagenomic Data Analysis
... Abstract Metagenomic approaches are a growing branch of science and have many applications in different fields. Metagenomics seems to be the ideal culture- independent technique for unraveling the biodiversity of soils ...
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Next Generation Sequencing
... In the first phase, raw reads are mapped to generate SAM files and an accurate base error model is used to improve on the vendor’s base quality calls (which are not very accurate). In ph[r] ...
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