High Throughput (NGS) DNA sequencing
THetA: inferring intra tumor heterogeneity from high throughput DNA sequencing data
... from high-throughput DNA sequencing data, in the case where copy number aberra- tions distinguish ...real sequencing data - demonstrating the ability to identify intra-tumor hetero- ...
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CODEX2: full spectrum copy number variation detection by high throughput DNA sequencing
... Abstract High-throughput DNA sequencing enables detection of copy number variations (CNVs) on the genome-wide scale with finer resolution compared to array-based methods but suffers from ...
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High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia
... of high throughput DNA sequencing and bioinformatics tools provide a broadly available and increasingly cost ef- fective method to identify bacterial populations found in polymicrobial ...
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Optimizing a MapReduce Module of Preprocessing High-Throughput DNA Sequencing Data
... In this paper, we present three strategies to handle data communication between the Map() and Reduce() functions of the MapReduce framework in a bioinformatics application that detects and corrects sequencing ...
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Application of High-Throughput DNA Sequencing Technologies in the Metagenomic Study of Marine Biofilms
... parallel sequencing (next generation sequencing, NGS) technologies in mid 2000s initiated a renaissance in microbial ecology by allowing the in situ investigation of environmental samples at metagenome ...
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High-throughput DNA sequencing laboratories
... relatively high percentage of overinjections experienced with the LPA matrix when excess DNA is injected, impeding the flow of current through the capillary and pro- hibiting bands from reaching the ...
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Microbial Oceanomics using High-Throughput DNA Sequencing
... Sanger publishes “chain-terminator” method for DNA sequencing 1987 First commercial sequencing machine (ABI 370).. 1995 First genome of a free-living organism (bacteria).?[r] ...
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Efficient storage of high throughput DNA sequencing data using reference-based compression
... We have implemented our algorithms in a prototype written in the Python programming language. The source files are available at http://www.ebi.ac.uk/~markus/mzip. This prototype works with BAM based input, but it has ...
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Efficient storage of high throughput DNA sequencing data using reference-based compression
... Our strategy is to pool unmapped reads from a variety of ‘‘similar’’ experiments, e.g., those using the same individual or species, and then discover runs of sequences that are present across the experiments. These ...
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Genetics of non-syndromic childhood obesity and the use of high-throughput DNA sequencing technologies
... exome sequencing are important diagnostic tools to identify new genes and variants associated with severe obesity, but other approaches are also useful at individual or population levels, such as linkage analysis, ...
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Process Improvement as a Catalyst for Innovation, Examples from High Throughput DNA Sequencing. Robert Nicol Director, Sequencing Operations
... Cell Circuits Program Genome Sequencing Platform. Chemical Biology[r] ...
Fine grained compositional analysis of Port Everglades Inlet microbiome using high throughput DNA sequencing
... possibly creating favorable bacterial growth conditions. Potentially pathogenic genera were present in the PEI. However their relatively low, non-significant abundance levels highlight their relatively low risk for ...
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Spectrum of mutations in monogenic diabetes genes identified from high-throughput DNA sequencing of 6888 individuals
... in high-throughput sequencing technologies have made it possible to sequence selected regions of the human genome in large numbers of indi- ...targeted sequencing approaches have been utilized ...
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High Throughput Next Generation Sequencing of Polioviruses
... Sanger-based sequencing of the viral protein 1 (VP1) capsid region is currently the standard method for PV ...whole-genome sequencing protocols for poliovirus isolates and FTA cards using next-generation ...
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Methodology for Copy Number Variant Detection from High. Throughput DNA Exome Sequencing and Application to the
... Generation Sequencing Both CGH and SNP approaches to detecting CNVs predate the development of Next Generation Sequencing ...(NGS). High throughput technologies allow high genomic ...
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High throughput sequencing to decipher the genetic heterogeneity of deafness
... NGS has already had an important impact on both research and clinical diagnosis in other diseases, such as breast cancer [5], intractable infl ammatory bowel disease [6] and Kabuki syndrome [7], as it enables screening of ...
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Bayesian modelling of high-throughput sequencing assays with malacoda.
... function. High-throughput screens powered by NGS have greatly increased the rate of vari- ant functionalization, but the development of comprehensive statistical methods to analyze screen data has ...by ...
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High-throughput sequencing of cytosine methylation in plant DNA
... bisulphite sequencing (RRBS) ...deeper sequencing in CpG-islands than would otherwise be pos- ...a high number of ...Targeted sequencing methods [35], in which specific portions of the genome ...
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Indices and Applications in High-Throughput Sequencing
... and sequencing error rates of different high-throughput sequencing technologies by mid 2011 [Glenn, ...The DNA template is irst replicated multiple times (ampli ication) and then ...
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Mapping DNA structure & protein-DNA interactions using hydroxyl radical footprinting & high-throughput sequencing
... genomic DNA, can thus help clarify OH-seq ...the sequencing coverage obtained with this method is rather uniform across the whole genome, especially at higher radiation ...protein- DNA interactions ...
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