Histone (H3-H4)2
Histone deacetylases 1 and 2 maintain S-phase chromatin and DNA replication fork progression
... treatment with 898 compared to DMSO alone (Figure 9A, compare lanes 6 and 7 to 2 and 3). Similar results were obtained following knockdown of Hdacs1,2 when com- pared to the control (Figure 9B, compare lanes 6 and ...
22
Global Replication-Independent Histone H4 Exchange in Budding Yeast
... mutant histone chaperone strains ...replication-independent histone exchange ...(Fig. 2 and 3). As such, our data indicate that replication- independent H4 histone exchange occurs ...
8
Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote
... JMJD2C histone demethylase ...as H3/H4 dimers, assembled with “new” unmodified H3/H4 dimers ...free H3 histones or on newly assembled H3/H4 dimers, appears to ...
19
Construction of Comprehensive Dosage-Matching Core Histone Mutant Libraries for Saccharomyces cerevisiae
... and 2 above, it is notable that the charge neutralization mutants at the same residues are ...among histone–histone interaction surfaces (Figure 3F and Figure S6 in File ...disrupting ...
11
A Simple Method to Produce Sub Nucleosome Complexes of High Purity In Vitro
... even H3/H4 tetramers could be in vitro reconstituted directly from 10 mM HCl (Figure 3(c)) without urea denaturation by this method which was not previously ...against 2 M NaCl leads to precipitation ...
9
Tousled kinase TLK1B counteracts the effect of Asf1 in inhibition of histone H3–H4 tetramer formation
... [8], histone H3 [9], and more recently Rad9 [10], which suggested a function in chromatin assembly [11] with implications in transcrip- tion [12,2], condensation of chromosomes [4], and DNA repair ...
5
Dissecting The Molecular Basis Of The Scaffold Protein Jade In Hbo1 Histone Acetyltransferase (hat) Activity
... multi-protein histone acetyltransferase (HAT) complexes containing ING4/5, MEAF6 and scaffolding proteins JADE1/2/3 or BRPF2/3 to acetylate histone H4 H4K5/8/12 or H3K14, ...for histone ...
114
HAT1 signaling confers to assembly and epigenetic regulation of HBV cccDNA minichromosome
... DTT, 2 μg/ml aprotinin; 2 μg/ml leupeptin and 1 mM ...DTT; 2 μg/ml aprotinin; 2 μg/ml leupeptin and 1 mM ...of histone H3K27, H4K5 and H4K12 were measured by Western blot analysis in ...
14
Acetylated Histone H3 and H4 Mark the Upregulated LMP2A Promoter of Epstein-Barr Virus in Lymphoid Cells
... of histone acetylation in the regulation of ...methylation, histone acetylation, and H3K4me2 on the regu- lation of LMP2Ap, as LMP2A could be induced with TSA or ...
6
Maintenance of Heterochromatin by the Large Subunit of the CAF-1 Replication-Coupled Histone Chaperone Requires Its Interaction with HP1a Through a Conserved Motif
... stage 2 – 5 in which the synaptonemal complex, maintaining aligned homologs, is fully assembled; and the middle region containing egg chambers from stage 6 – 10, in which the synaptonemal complex is undergoing ...
24
Völker-Albert, Moritz (2017): Proteomic analysis of assembled chromatin with SWATH-MS. Dissertation, LMU München: Medizinische Fakultät
... Nuclear-enriched fractions were separated by SDS-PAGE, stained with Coomassie (Brilliant blue G-250) and protein bands in the molecular weight range of histones (15-23 kDa) were excised as single band. Gel slices were ...
165
JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability
... Given that siJMJD1B cells are affected on histone deposi- tion, we investigated whether siJMJD1B cells have signs of cell cycle arrest and DNA damage. We first analyzed the impact of the JMJD1B knockdown on the ...
15
Epigenetic effects of RRx-001: a possible unifying mechanism of anticancer activity
... 5-aza- 2’-deoxycytidine (decitibine), have already been approved by the Food and Drug Administration (FDA) as effective drugs for treatment of patients with myelodysplastic syndromes ...of histone ...
10
Mechanisms of suberoylanilide hydroxamic acid inhibition of mammary cell growth
... a histone deacety- lase inhibitor and caused accumulation of hyperacetylated histone H4 in murine erythroleukemia ...in histone H3 and H4 protein and acetylation ...
12
The conformational flexibility of the C-terminus of histone H4 promotes histone octamer and nucleosome stability and yeast viability
... Samples were processed as previously described [65]. Briefly, 2 × 10 8 asynchronous cells were harvested and washed before spheroplasting with Zymolyase 100 T at room temperature. Spheroplasts were washed twice ...
20
The targeted histone deacetylase inhibitor tefinostat (CHR-2845) shows selective in vitro efficacy in monocytoid-lineage leukaemias
... Although mechanisms of action of HDAC inhibitors remain to be fully characterised, sensitivity to these agents has been linked to the apoptotic capability of each cell type. Differing combinations of apoptotic mediators, ...
13
Koreski_unc_0153D_18349.pdf
... the H3–H4p are required for HLB formation, and retargeted CLAMP recruits HLB ...wild-type histone gene cluster (A) or a cluster in which the GA repeats are replaced by two LacO sites (B) into the Drosophila ...
117
Original Article The toxic effects of a plasticizer, dibutyl phthalate, on rat testis
... of H3 histone in spermatogonial cells of con- trol group was much stronger than that in spermatic cells (Figure ...ble 2). Besides, the brown sig- nal of H3 histone in sperma- togonial ...
8
Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter
... matin from uninfected mice (Fig. 2A, lanes 1, 4, and 7) or after immunoprecipitation of chromatin from infected mice with nonspecific IgG antibody control (Fig. 2A, lanes 8 and 9), demonstrating the specificities of our ...
10
Comprehensive analysis of histone post-translational modifications in mouse and human male germ cells
... for 2 h, and the subse- quent histone pellet was washed twice with cold acetone prior to resuspension in DNase/RNase free ...parts 2-propanol) at pH 8 to block the ɛ -amino groups of unmodified and ...
15