Housekeeping Genes
Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density
... reliable housekeeping genes that are not regulated under the different experimental conditions for normalization ...[11]. Housekeeping genes are constitutively expressed genes whose ...
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Genetic Diversity of Neisseria gonorrhoeae Housekeeping Genes
... gonococcal housekeeping gene ...the genes, consistent with evolution of DNA sequences by point ...keeping genes, positive selection also appeared to operate on the abcZ and gpdh ...of ...
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Housekeeping genes tend to show reduced upstream sequence conservation
... of genes. In particular, it has been observed that the promoters of genes encoding regulatory proteins, such as transcription factors and/or developmental proteins, tend to show remarkably strong sequence ...
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<p>Nanoparticles Affect the Expression Stability of Housekeeping Genes in Plant Cells</p>
... reference genes for the qRT-PCR analysis of gene expression in NP-treated plant cells has not been ...nine housekeeping genes—ACT2, ACT3, ACT7, EF1-α, GAPDH, Histone H2A (H2A), TUB-α, TUB-β, and ...
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Defining housekeeping genes suitable for RNA-seq analysis of the human allograft kidney biopsy tissue
... of housekeeping genes (HKG) is essential for further progress in this ...as genes ubiquitously expressed in all tissue compartments and cell-types regardless of their developmental stage, ...
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Mining housekeeping genes with a Naive Bayes classifier
... of housekeeping and tissue specific genes for various attributes of chromatin organi- zation ...specific genes as compared to house- keeping ...loops. Genes less frequently expressed (tissue ...
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Age related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues
... reference genes for normalization of gene expression analysis in some cases ...identify genes with stable expression preferably across cell types since many qRT- PCR studies are performed on cDNA isolated ...
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Mycobacterial SigA and SigB Cotranscribe Essential Housekeeping Genes during Exponential Growth
... found in close proximity to known TSSs (Data Set S3) (8, 50, 51); these included experimentally determined ⫺ 10 sequences published previously, such as those for ideR, rpsL, rrnP, and sigA (52–54). Curiously, however, ...
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Translational selection in human: more pronounced in housekeeping genes
... expressed genes in human ...TS genes in all examined tissues (Figure 2), we further investigated whether the stronger correlation between CUB and expression level in EIGs is caused by high expression ...
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Reduced Susceptibility to Antiseptics Is Conferred by Heterologous Housekeeping Genes
... of genes in the oral microbiome conferring reduced susceptibility to common ...The genes responsible for this phenotype were identified as fabI, originating from a variety of different ...
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An iteration normalization and test method for differential expression analysis of RNA-seq data
... Human housekeeping genes, as described in [16], were downloaded from [17] and matched to the Ensembl gene identifiers using the Bioconductor [18] biomaRt package ...of genes including 421 of 538 ...
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Use of Whole Genus Genome Sequence Data To Develop a Multilocus Sequence Typing Tool That Accurately Identifies Yersinia Isolates to the Species and Subspecies Levels
... 84 housekeeping genes that are located on 23 syntenic blocks, regions of DNA- containing genes conserved across the genus, and showed the presence of 14 species clusters as determined by Bayesian ...
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mRNA degradation: an underestimated factor in steady state transcript levels of cytochrome c oxidase subunits?
... Steady-state mRNA levels are determined by synthesis and degradation; however, changes in mRNA levels are usually attributed to transcription. For cytochrome c oxidase (COX), cold acclimation typically leads to an ...
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Delineation of Streptococcus dysgalactiae, Its Subspecies, and Its Clinical and Phylogenetic Relationship to Streptococcus pyogenes
... other genes, both previous studies showed a close relationship between ...the genes rpoB, tuf, pyk, and especially ...rpoB genes and identical alleles of the tuf ...of housekeeping ...
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Characterization of Sucrose Negative Pasteurella multocida Variants, Including Isolates from Large Cat Bite Wounds
... the housekeeping genes rpoB and infB point to the existence of a new sucrose-negative species of Pasteurella iso- lated mainly from the wounds of bites caused by large cats and tentatively named taxon 45 of ...
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Draft genome sequence of the type strain of the sulfur-oxidizing acidophile, Acidithiobacillus albertensis (DSM 14366)
... these genes (6–14%). For the rest of the flagellar genes the rate of SNPs conductive to amino acidic substitutions be- tween ...chemotaxis genes were predicted in the ...of genes is organized ...
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Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage
... of housekeeping genes, including gapdh, sdha, s18 and actb, but these genes appeared to be selected based on what others had used in similar studies and not because they had been selected as the most ...
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Complete genome sequence of Rhodothermaceae bacterium RA with cellulolytic and xylanolytic activities
... the housekeeping genes to the other members of the family Rhodothermaceae, strain RA has been assigned as an unclassified taxon of the family Rho- dothermaecae, order Bacteroidetes Order ...
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Multilocus Sequence Typing and Phylogenetic Analysis of Propionibacterium acnes
... nine genes, offers significantly en- hanced resolution and phylogenetic inferences more concordant with analyses based on a comprehensive sampling of the entire genomes, their gene contents, and their putative ...
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Cross Sectional and Longitudinal Multilocus Sequence Typing of Pseudomonas aeruginosa in Cystic Fibrosis Sputum Samples
... several housekeeping genes. House- keeping genes are chosen because they are assumed to be under low selection pressure and therefore to have a low mo- lecular clock speed, ...the housekeeping ...
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