Human Embryonic Stem Cell-Derived Cardiomyocytes
Mathematical modelling of the action potential of human embryonic stem cell derived cardiomyocytes
... from human and animal CMs represented a compulsory choice for our model, since some current properties expressed in the adult ventricular cells were unknown or not suffi- ciently characterized in ...bryonic ...
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MicroRNA-363 negatively regulates the left ventricular determining transcription factor HAND1 in human embryonic stem cell-derived cardiomyocytes
... UCSF Stem Cell Research Oversight Committee ap- proved all experiments with ...BCiPSP16 human induced pluripotent stem cells (hiPSCs, a gift from ...mouse embryonic feeder cells (MEFs) ...
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Metabolomics Identifies Metabolic Markers of Maturation in Human Pluripotent Stem Cell-Derived Cardiomyocytes
... H9 human embryonic stem cells were differentiated to cardiomyocytes via the GiWi directed differentiation protocol [2–4] and maintained in culture in RPMI supplemented with B27 on ...
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A defined synthetic substrate for serum free culture of human stem cell derived cardiomyocytes with improved functional maturity identified using combinatorial materials microarrays
... from human stem cells have applications in regenerative medicine and can provide models for heart disease and toxicity ...diomyocytes derived from human embryonic stem cells ...
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Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes
... hiPSC cell lines, and delineate mechanisms that promote CM com- mitment and ...immaturity. Human PSC-CMs are most similar to CMs obtained from embryonic or fetal ...
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Deep phenotyping of human induced pluripotent stem cell–derived atrial and ventricular cardiomyocytes
... the human heart. Adult CMs differ from iPSC-CMs with respect to shape, cell size, cellular structures, and metabolism by being far larger, rod-shaped, with large sarcomeric area, and pos- sessing an ...
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Engineered Microenvironments for Maturation of Stem Cell Derived Cardiac Myocytes
... of cardiomyocytes in vivo during embryogenesis ...and human embryonic stem cells (hESCs) were induced to differentiate with ~35% success ...enough cardiomyocytes of adequate quality ...
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Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes
... healthy human car- diac tissue ...of cardiomyocytes by using noninvasive impedance readout and obtain quantitative data by converting impedance into cell index values ...native human heart ...
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Perspectives on the Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Biomedical Research
... of stem cells to cardiomyocytes and vascular cells mimics the sequential stages of embryonic cardiac development (for review see Birket and Mummery, ...endoderm cell layers in the primitive ...
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Human embryonic stem cell-derived cardiomyocyte therapy in mouse permanent ischemia and ischemia-reperfusion models
... in cardiomyocytes, cardiac function was improved due to decreased neutrophil recruitment to the heart ...when cell therapy is purposed to treat ischemic heart, the microenviron- ment in the heart such as ...
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Loss of beta1 integrin function results in upregulation of connexin expression in embryonic stem cell derived cardiomyocytes
... recombinant human leukaemia inhibitory factor (LIF; see (Fässler et al ...into cardiomyocytes, wt and β1-/- cells were cultivated as embryoid bodies (EBs) in hanging drops for 2 and in suspension for 3 days ...
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Developing A Preclinical Model Of Human Sunitinib Cardiotoxicity To Assess The Role Of Mechanical Loading Using Engineered Cardiac Microtissues
... of cell signaling networks allowed researchers to elucidate the differences between healthy and cancerous ...normal cell function, regulating processes associated with growth and differentiation, and are ...
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High glucose suppresses embryonic stem cell differentiation into cardiomyocytes
... A total of 30–50 μg protein from cells at differentiation day 9 was extracted using the cell lysis buffer (Cat# 9803, Cell Signaling, Danvers, MA, USA) containing a 1% protease inhibitor cocktail ...
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A Study of the Role of Gata6 in Definitive Endoderm Specification and Β-Cell Functionality by Genome Engineering of Pluripotent Stem Cells
... the human phenotype. Induced pluripotent stem cells were created from a pancreas agenesis patient with a heterozygous mutation in ...additional stem cell lines with mutations in both GATA6 ...
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Materials for stem cell factories of the future
... Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.. Derivation of human embryonic stem cells in defined[r] ...
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Are Stem Cells the next Therapeutic Tool for Heart Repair?
... mesenchymal stem cells genetically enhanced with Akt1 can repair infarcted myocardium, prevent remodeling and nearly normalize cardiac performance [ ...other stem cells are used as well, as recently shown ...
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Highly efficient differentiation of neural precursors from human embryonic stem cells and benefits of transplantation after ischemic stroke in mice
... respectively [40]. In the present study, we examined the expression of SOX1, another transcription factor indicated in the specification of early neural cell fate [52]. This marker was expressed in 64% ± 9% of ...
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GLUT4 expression and glucose transport in human induced pluripotent stem cell-derived cardiomyocytes
... this cell type, strengthens the suggestion that iPSC-CM may exhibit expression of GLUT transporters (and general physiology) analogous to foetal ...this cell type with notable functional ...
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Bigler_unc_0153D_17288.pdf
... single cell pharmacological manipulation they ruled out involvement of postsynaptic ...presynaptic cell they compared the effect of the large molecule translation inhibitor gelonin, which has limited ...
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Tissue-engineered cardiac patch seeded with human induced pluripotent stem cell derived cardiomyocytes promoted the regeneration of host cardiomyocytes in a rat model
... of stem cell or progenitor cell ...that cell transplantation upregulates various cardioprotective factors in native cardiac tissue through “crosstalk” between transplanted cells [12, ...host ...
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